Gene:
Gene Symbol:
IST1
Gene Name:
increased sodium tolerance 1 homolog (yeast)
Original Clone ID:
WISP 14-25
Keyword:
"Mutation:c162a; a125c, Mutation:D42A, IST1 1-189 D42A Sundquist Lab Internal ID corresponding to this clone = WISP 14-25, 1STI N-terminus (aa 1-189) D42A"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEX2T(TEV)
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
John McCullough
Leremy A Colf
Wesley I. Sundquist
John McCullough
Leremy A Colf
HsCD00671563
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 570nts
Open reading frame: 1 to 570
ATGCTGGGCTCTGGATTTAAAGCTGAGCGCTTAAGAGTGAATTTGAGATTAGTCATAAAT
CGCCTTAAACTATTGGAGAAAAAGAAAACGGAACTGGCCCAGAAAGCAAGGAAGGAGATT
GCTGCCTATCTGGCTGCTGGGAAAGATGAACGAGCTCGGATACGTGTGGAGCACATTATC
CGGGAAGACTACCTCGTGGAGGCCATGGAGATCCTGGAGCTGTACTGTGACCTGCTGCTG
GCTCGGTTTGGCCTTATCCAGTCTATGAAGGAACTAGATTCTGGTCTGGCTGAATCTGTG
TCTACATTGATCTGGGCTGCTCCTCGACTCCAGTCAGAAGTGGCTGAGTTGAAAATAGTT
GCTGATCAGCTCTGTGCCAAGTATAGCAAGGAATATGGCAAGCTATGTAGGACCAACCAG
ATTGGAACTGTGAATGACAGGCTAATGCACAAGCTGAGTGTGGAAGCCCCACCCAAAATC
CTGGTGGAGAGATACCTGATTGAAATTGCAAAGAATTACAACGTACCCTATGAACCTGAC
TCTGTGGTCATGGCAGAAGCTCCTCCTTAG
Protein sequence predicted based on nucleotide sequence
MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIAAYLAAGKDERARIRVEHII REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV ADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPD SVVMAEAPP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEX2T(TEV)
Synonyms:
''
Description:
Bacterial Expression vector with an N-terminal GST tag and TEV site; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 01-69; Nde1, BamH1 restriction enzyme cloning can be used easily with this vector
Size (bp):
4993
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
primer
forward primer
pGEX forward sequencing primer GGTTCCGCGTGGATCTCCGG
920
939
primer
reverse primer
pGEX reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG
1043
1065
promoter
tac promoter
tac promoter
180
209
protease cleavage site
TEV
N-terminal TEV protease cleavage site
0
0
reporter gene
LacZ
LacZ alpha
4655
0
repressor binding site
lac operator
lac operator binding site
215
238
selectable marker
AmpR
Ampicillin resistance
1599
2259
tag
GST
N-terminal GST tag
258
929
