Gene:
Gene Symbol:
VPS4
Gene Name:
AAA family ATPase VPS4
Original Clone ID:
None
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pET151-D-Topo
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
"Mutation:a481g;g482c;a483t, Mutation:R161A, Vacuolar protein sorting-associated protein 4 (Vps4) residues 81-437 (end); lacks MIT domain; mutation R241A; internal ID CPH2575 (pET151-D-Topo-Vps4p(81-437,R241A)), pore loop mutant, defective in substrate binding"
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Han Han
Christopher P. Hill
Wesley I. Sundquist
Han Han
Christopher P. Hill
ScCD00671616
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1074nts
Open reading frame: 1 to 1074
GCGGCAAAGAAATCTCCTAGCGCTGGTAGTGGTTCCAACGGTGGTAATAAGAAAATCTCT
CAGGAAGAGGGTGAGGATAATGGTGGAGAAGACAACAAAAAGCTAAGAGGTGCCTTATCG
AGTGCTATTTTGTCTGAGAAGCCCAATGTTAAGTGGGAAGACGTGGCTGGACTGGAAGGA
GCCAAAGAGGCCCTTAAAGAAGCCGTTATTTTACCTGTCAAGTTCCCACATTTGTTCAAA
GGTAACCGTAAGCCCACTTCGGGAATCTTATTGTACGGACCACCAGGTACAGGTAAATCA
TATTTGGCAAAAGCAGTGGCCACCGAGGCCAATTCTACCTTTTTCAGTGTTAGTTCCAGT
GATTTGGTTTCTAAGTGGATGGGTGAATCTGAAAAACTTGTCAAACAGTTATTTGCGATG
GCAAGAGAAAATAAACCTTCTATTATTTTTATAGATGAAGTGGATGCGCTAACAGGTACC
GCTGGAGAAGGAGAGAGCGAAGCAAGTAGAAGAATTAAAACAGAATTATTGGTACAAATG
AATGGTGTTGGGAATGACTCCCAAGGTGTTTTGGTCCTTGGCGCAACAAATATCCCATGG
CAACTGGACAGTGCCATCAGGAGAAGATTTGAAAGAAGAATATATATACCATTACCAGAT
TTGGCGGCAAGAACCACCATGTTCGAGATTAACGTCGGCGACACTCCATGCGTATTGACC
AAGGAAGATTATAGAACTTTAGGTGCAATGACTGAAGGTTATTCCGGGAGTGATATTGCT
GTAGTGGTGAAAGATGCGCTAATGCAACCGATAAGAAAAATTCAAAGCGCGACACATTTC
AAAGATGTCTCTACGGAGGACGACGAAACAAGAAAACTAACACCATGCTCTCCAGGTGAT
GATGGCGCCATCGAGATGAGTTGGACGGATATTGAAGCTGATGAATTAAAAGAACCAGAT
CTCACCATAAAGGATTTCTTAAAGGCTATCAAATCGACGAGACCTACCGTGAATGAGGAT
GACTTGCTGAAGCAAGAACAGTTCACTAGAGATTTTGGTCAAGAAGGTAACTAG
Protein sequence predicted based on nucleotide sequence
AAKKSPSAGSGSNGGNKKISQEEGEDNGGEDNKKLRGALSSAILSEKPNVKWEDVAGLEG AKEALKEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSS DLVSKWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGTAGEGESEASRRIKTELLVQM NGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTPCVLT KEDYRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHFKDVSTEDDETRKLTPCSPGD DGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQEGN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET151-D-Topo
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and a TEV cleavable N-terminal 6xHis and V5 tag; amipcillin resistance in bacteria; TOPO cloning
Comments:
None
Size (bp):
5760
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
387
400
bacterial origin
pBR322 origin
pBR322 origin
1953
2626
promoter
T7
T7 promoter
209
225
protease cleavage site
TEV
TEV protease cleavage site
360
380
repressor protein gene
LacI
LacI gene
4500
5612
ribosome binding site
RBS
Ribosome binding site
282
289
selectable marker
AmpR
ampicillin resistance gene
948
1808
tag
His
N-terminal 6xHis tag
300
317
tag
V5
N-terminal V5 epitope tag
318
359
trxn regulatory element
lac operator
lac operator (lacO)
228
252
trxn termination sequence
T7 term
T7 termination
416
544
