Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Sequence Verified: Yes; 3'UTR length: 2158; Forward Primer: AAGTTCCTGAACATGATGACCTAG; Reverse Primer: GTTCTGACCCTAGGAGTGTTTGGG
Species:
Homo sapiens
Type:
3'UTR
Vector Name:
P2RP3
Format:
None
Source:
Center for Personalized Diagnostics
Description:
Please cite: Kotagama, K., Babb, C.S., Wolter, J.M., Murphy, R.P. and Mangone, M. "A human 3'UTR clone collection to study post-transcriptional gene regulation". BMC Genomics 16, 1036 (2015).
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
Authors:
Center for Personalized Diagnostics
Marco Mangone
Kasuen Kotagama
Marco Mangone
Kasuen Kotagama
HsUT00699345
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2157nts
Open reading frame: 1 to 2157
GGAGAGCAAGTTCCTGAACATGATGACCTAGAGGCCCGAGCGCCTGGTGCTGAGTGCACC
CGGAGGGGTCATCGCTGCCCAGAGCCTGGGGATTCCAGCTGTCTTGTCTTTTTCCAAAAA
AGTGTTAAATAGGCTTGAGGGGTTGTTGCGCAATGGCCGCTTCAGATGACAGATGTTGTA
AGAGAAGGTTTATGGGCATCCTCTCTGGTCTTTTGGATTATTCCTCAGAACAATGAAAAA
AGTCTCCATAGGACAGGAAGGAATGCAAAACTCATTTACACAGTGCTTTCCAGCCTTTGG
TGCTTACAGGACCGCGCTGTTCCGGCTTCTTCACGGCTGACATTCGGCTAACGAGGGATT
ACTTTGGCCAAAACCTTTCAAAGGATATGCAGAAAGATGGTAGGGAGCATTTGGGTTTGA
ATCTGAATGCTATACTGGATACTCTGCTCCGGAAAGATGAGCTTTTTATTCTACTACTTG
GAAGGAAAAGGAATTCCTGGTCCACCTGAATTCCTCTATGAAGCCTAACTCTTGAGGTCT
CTAACATACCTTGTCATAGAGGAAAAGCACAGATTATACCTGGATGATTCAGGAGCACAT
TCTGATTCCAGGTTTGGTAGAGCTGGCTCTTCTACTCCGTAAAGCCGAGTCTGGGACTGG
CAGCCCATCCAAGTGTATATGAATGAATAAAGCATCCAAGTATATATGAATGAATAAAGT
ATGTAAGTATCACCAGAAAAAGGAAAGAAAAAATGTACTCCTTGGGGCAAGCCCAGAAGC
TGCCCTGGCCTCTCCAGACCGTGTTTACAGTGTTTGCATGTAGAATGTAGCCCTTCCTGA
AAAGAAGACTTGTTTCTAAATACCTCGGGGCTGCTGGAGCCGCTGTGGGTTAGGGATGGA
CTGAGGCCTCGAGGAGTGAGGGTGCACCCGGGGCCCAGCCTCAGGCTGCCCTAGGGATCT
CTCAGTAGGAAGAGGAAGTTGCGTGTTTACCCAATCCTGTTTCTCCAATGCAACGTCCAC
CCACTTTACCACCAAAAACTCCAGGGCCTGACGGCAGCCCGGTCCCCCAGCACTCACCAG
CAGCCCAGTGTTCTCCACCAAGCCACAGTGTGCATGCCTGGTATCCTCCGGATTCCCTTC
CTTCTGCCCGCTGAGTCACTGGGCAGAGAATGATGACATGTGTAGGTGGTGTGGTTGGGG
GTGGAAAGGGGAAGGGGTTGATCCTCAGGACTCTGAGGGAGCATCGTTGAATTTTCCTGT
TCAGTGTGACCAAGACCCACCTGGAAATGGAATTTGGAACTGGCTTCAGGAGACATCATT
CCTGAACACACTGTAGGGTGAATTGGTGCATCTTCCCCACCATACACACACACACACACA
CACACACACACACACACACACACACACCCCAAACCTTTTCATGGGGAATGTGTGGCAACC
TTGCCAAACAGCACCACTCAGAGTGTGACTCTGACTGTGACCTTGGCCTTAATGAGGAAC
TTCTTAGGAGAGTTTGAGGACAAGGCCAACATCGTCATCTGGGCTCGCTGCGTCCCAGCA
CATCAAACTCTGTCCAGAGACAAGGCCAACTGCAAATGAAAGCCAGGGAACATTGCTAAG
GGTCTGTGGCTCTGTGGTGGTGTTCATCGCCTTCCTGAGATAGGATTTCCCTTGCCAGTC
CCAACCTGTATATATTCTGTACAGAAGACATCCCTGAATATACTGTAGGTGAGTCGTCCA
GCCAAATTTATATCTCCAAAACATTTTTAGCTTTTTCTACATGCTATGAATTGAGATGAC
ATGCTCAACTTGTAAATAAGTCTTTTTGTACATTAAAAAAGTAATTTTTTCATAATTTAT
CTTGTCTATCTGCTTCCCCCTTGACAGTAGTTAATGAGAACCTGGGCAGTAAATTTGGTG
CATTCGAGCAGAAATTAGGCTGTATTTTTTCTTAACAGTGTCAAAATTGACTATCCCGCC
TTTGCCAAGAAATGTTTAATGCTGAGGCATTTACTGGCTGTTTGTTTGTTGTCTCCTTGG
GGCCGGGCAGGGTTTGACATTTCACAGTGAATATCTGACTGCTCTTCCGGTGAGCCACTC
TGAACATCTATATGCATTGCAGACCGGGGTATAGACCCAAACACTCCTAGGGTCAGAAC
Protein sequence predicted based on nucleotide sequence
GEQVPEHDDLEARAPGAECTRRGHRCPEPGDSSCLVFFQKSVK*A*GVVAQWPLQMTDVV REGLWASSLVFWIIPQNNEKSLHRTGRNAKLIYTVLSSLWCLQDRAVPASSRLTFG*RGI TLAKTFQRICRKMVGSIWV*I*MLYWILCSGKMSFLFYYLEGKGIPGPPEFLYEA*LLRS LTYLVIEEKHRLYLDDSGAHSDSRFGRAGSSTP*SRVWDWQPIQVYMNE*SIQVYMNE*S M*VSPEKGKKKCTPWGKPRSCPGLSRPCLQCLHVECSPS*KEDLFLNTSGLLEPLWVRDG LRPRGVRVHPGPSLRLP*GSLSRKRKLRVYPILFLQCNVHPLYHQKLQGLTAARSPSTHQ QPSVLHQATVCMPGILRIPFLLPAESLGRE**HV*VVWLGVERGRG*SSGL*GSIVEFSC SV*PRPTWKWNLELASGDIIPEHTVG*IGASSPPYTHTHTHTHTHTHTHPKPFHGECVAT LPNSTTQSVTLTVTLALMRNFLGEFEDKANIVIWARCVPAHQTLSRDKANCK*KPGNIAK GLWLCGGVHRLPEIGFPLPVPTCIYSVQKTSLNIL*VSRPAKFISPKHF*LFLHAMN*DD MLNL*ISLFVH*KSNFFIIYLVYLLPP*Q*LMRTWAVNLVHSSRN*AVFFLNSVKIDYPA FAKKCLMLRHLLAVCLLSPWGRAGFDISQ*ISDCSSGEPL*TSICIADRGIDPNTPRVR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
P2RP3
Synonyms:
''
Description:
Features:
ccdB – Bacterial toxin (suicide gene)
M13 reverse – Primer binding site
M13 Forward – Primer binding site
T7 – Promoter
CAT – Promoter
attP2 – Recombination site
attP3 – Recombination site
CmR – Resistance Chloramphenicol (Selectable marker)
KanR – Resistance to Kanamycin (Selectable marker)
Comments:
CPD only
Size (bp):
4773
Parent Vector:
None
Properties:
Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
Death Cassette
2084
2389
primer
M13 forward
537
553
primer
M13 reverse
3038
3054
promoter
T7
3015
3033
promoter
CAT
980
1082
recombination site
attP2
591
822
recombination site
attP3
2746
2977
selectable marker
CmR
Chloramphenicol resistance
1083
1742
selectable marker
KanR
3167
3967
