Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGCTTCTGATGAAATCTGGC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGGGTACTTCCCTTCCTG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708199
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7242nts
Open reading frame: 1 to 7242
ATGAGGGTACTTCCCTTCCTGGCTCTCACTGTCGCCTGCGTCTTCAGCACTGGGTCTGGA
GATAATGGAGATTGCTTCTTCTATGATCAGGTCTACAAAGATGGAGATACTATTAAATAT
GATTGCCAAATCTGTGAATGTACCAATGGAAGAATTACAGACTGTGCACGTGATGCGAAC
TGCATCTTTAAAAAGGTGGCTACACCTGAGCAGAATACTGATGCGATAGTCTATGATGAT
GATGATCCAAATATCATTGAAAGCAGCGAGAGCAAGGGGAATGTTCGAGCGAAACGGGCT
GCGTCTGGAGGACATAAGAAGAACCCCAGCGCTTATGAGACAAACAATAAATTCAAGCCA
AACCAAAGCAACCGTAATGATAATGATGACTCCAGCAAGGAACACAAGGATAGTAACAAA
GGGAAAGGACAGAAAATCAACAATGTATTGTCAAAGGATTCATCTGAAGAAAATGCATCT
AAAGAAAAATGGAGTCATGAAAAGAATGATAAAATGAAGACAAATGAAAGAAAGGGAAAA
GATCACAATGACTCCAGTAAGGAAAGTAAGGAGAAAAACCATGAGAAGAGACAGAATAAC
AAGAATGGATGGCTAAAGGACTCATCTGAAGAAAACGACAGTGCATCCAAAGAAAAGAAG
GATCATGACAAGAACAACAAAGTAAGGACAAATCAAAACAAGGGCAATGATCACGATCAC
TCCAGCAAAGAAAGCAAGGAAAATAGCCACGAGAAAGGAGAGAAAAGCAAGAAAAGATGG
CAAAATGGCTCAGCTGAAGAAAAAGATGATTCGTCCAAAGAGAAAAAGGATCATGAGAAG
AATGACAAAGTAAAGCCAAAACATAATAAGGGCAACGGTCATGATGACTCCAGCAAGGAA
AGCAAGGAAAATAGCCACGAGAAAAGAGAGAAAAACAAGAACAGGTGGCAGAAGGGCTCA
GCTGAAGAAAAAGATGATGGCTCCAAAGAAAAAAAGGATAAGACCCTAGAGGAGATGGAT
ACAAATGCATGCATATTTAATAAGGAATCTATAGCTGATGGGGAAACCAAAGATCTCAGC
TGTTCCACCTGCACCTGTAACAAAGGGAAGCTCACTTGCGTAAAGAACCTGAACTGTCCA
GGTGTCTGCTCAGTCACAGGTTATCAGATGGTTCGTACCTTTGATGGTTCCTTGTATCAA
AGCCCTGGAACCTGCAATTATATTTTGGTCAAGACCTCCGACTTCACAATTAGCCTTAGC
AACAAGGCCTGCTCTGATCAGAACCCAGATGCAGTTTGCATTGACACAGTGGACATTTAC
GTGCCTAACATTGCCAGCATTAAGGTTCTACAAGATGGATCAGTACTGTCAGCAGGGCAA
AAAGTTGACCTTCCGTTCTTTCTTCATGAATCTATTACTGTGAGAAGAAGTTCTTCTACG
TTCCTGGATGTGGTCACATCAATGTTCAGCCTGCAGTATGACATCGAGGGAAACCGACTG
TATGTCATCATTGAAACAAGCTACAAAGATCAAACCAGTGGGCTTTGCGGGACATACAAC
GACAATCGATTTGATGACTTCAGGAGCTCAAGTGACATAACTGAAACGGTGTCTAGTTTG
TTCAGCAAATCCTGGAAGTTGCAGTCTCAGTGTTCAGAGCATTTCATAACACCAGTCAGT
CCAGCTCAACGAGTCACAGCGGATTTAAGCTGCACTAATTCCCTTCAGGACTCGATCTTC
GCTGACTGCAACCTTATCATTGACATTCACAACTTTCAGGCCTCCTGTTCCAACAGCATT
TATTATGGGAAAAATGTTGGCTTTTGCTCAGCCATGGCAGACTATGCTTATCGATGTGCT
TTGGCAGGCGTTTATGTACCTGTGAGTTCTGCATTCCCAGACTGCTTTGTTTCTTGTGAA
GGAGGCATGGTTCCCATTACAGAAAATCTCTTTACTCAGGATGATTGTGCTCAGCATTCT
TCAGAGATTTTAAAGATGGCAGTGAAGCTTCCATTGAGTGAAGGTTGTGTCTGTCCTAAT
CATTTATACTTTGATTCTACAAGAAATGAGTGTGTTGATAGTGAGGAATGTCCATGTTAT
AGCAGCAACCATGCATACAGAATTGGAGAGAACATCACTTTTTCCAATGGACAATCATGT
GCTTGTGAGAGGATAATGGCCTGCACTGATAATCCTGACATTCCTATAGACGAAGTTCCA
ACATGTCCAGATGATGAGGTTTTCTCCGATTGTTTGATGGGATCTGGAAAATCTTGCGAG
CCAAGCTGCCAAAACATGGCTGTCATTGACCAAGTCTGCCCTCTAGAGTGTGAGGCGGGC
TGCATCTGCAAATATGGAAAGTTGAGGAGCAATGACGGCACCTGTGTCCCACTGCAGGAA
TGTCCATGTGTGCATGGAGAAGATGTTTATAACCCTGGAGAGACACTGGCACAGGATTGC
AACACTTGCACATGCAAAGATGGCAAGTTCACATGCACCAATAATGCTTGCAACGTGGTA
TGTAATGCCTATGCTGGAGGCCAATTCATCCTCTTCGATAACATCTGGAAAACCTTCAAG
ACAGGGGAGTGTCAGATCATTCTTGTTGAATCTCAAGACGGGCAAGTCCCCAAATTCAGA
GTTGTAATGCAAAACACAAAAACTGAAAATCTTGGTGGAGCTTTGGCCACCAAGAAGATC
TTCATCAGATTTGGAGGAGCATATGTAGAGTTGTCCGATGCAGATCCTGTCTTGGTTCAA
GACTTGGGAAGTACAACCCAAGTAAGACTCTATCGTTCTGGATTCTACATTGTTGCCCAT
TTTCTGGAAGGTCTTGCAGTTTACTATGATCAGCACTTAGATGTTATTATTCAACTCCAG
CCTCATCTACAGGGCAAGGTCCAGGGTATGTGTGGTGATGCTGACCAGACAACAACTGCT
GAGCTAGCTATTAGCAACATGGCACAGTATGGAGCTCAGTTCATTATTGGCAAGTGCACT
TCCTCAGATGAACCCTCACCTCCTCCATCTGATAACAACAAGCATTACACAGAGAAACGC
TGCAGTTTGCTGTTCAGTCCGGACTTCGCTGAGTGTCACACCATTGTCCCTGTCCAACCA
TATTACACTGCTTGTGTAGAAGAGACCGAGGCCTGCAGGGAAGGAGAAAGCTGTCTGTGC
TTCTGTACAGCTCTTTCTGCTTATGCCCGAGCCTGCTGCCGTAAGGGCATCAACGTTGAC
TGGAGAAACCCCGACACATGCCCTTCCCCTTGTGAATACTACAACCGAGATTCTGGAGAG
GGACCATATCGTTTAGTCATGGTCAATGGCCAAACCTTGATGGCAGATTATGACACCCAG
TCTGTGTCACTGATCACTAAGGATACATCAGGAGATGTCAAAGTCAGCTTCATGGTCACC
CCAAGTCTCTTTGTGGACCCACTGTATGGCAGAAAGTTAATCTCTTTGGAATCTGCTCTG
CACCGCAACTTCTTCATCGTCCAGAATGCAGATGGTACCCTAGGCTTAAGGAAGTGGCAG
CCAAGTGCCAGCTTCCGTATACAGGCTACTTTTATCCTGAGAGAAGGTCGCTGGATAAAT
GGCTACAGCACTCTTGAATCCTATTCCTCTAGGGGCCAATATCTCTCTTTTAACAACACC
AACAAAGTTCTGGTGATGACCAGGGTTAAATCTACAAACATGCTGGCAATGAATTTCAAG
CTTACAGAGGAAACCTTTGGATTACCATCATTCTCTATCTGCACCTGGAAGTACAGATCT
TGTGGCCCTGCTTGCATCCCAACATGCCAAGATCCCCTGGGAGAGAAGTGCACCCTAACT
CTTAAAGTGGAGGGCTGCTTCCCAATTTGTGCCCCAGGAATGGTGTTCGATGAAGTTACC
CATCGTTGTGTGAATAAAGATGACTGTGTGTCCATCCCTACGGTATCACCAACACCAGTG
CCACCGACACCAACTAAGGAACCATGCAAGGATGTGACTTGTGTAGAAATGAATTGCAAG
GTTGGAGAAACTAGAGTGGAAATAAAAACAACAGATCCCTGCTGCCCACGTTATCAATGT
GAGGCCATTCCTACTACCCCAGCACCACCAACAACACCGAACCCATGTAAAGATGTCAAT
TGTGAAATTCCAACATGCAATTCAGATGAACAACTTGTACAAGTTAAATCAACAGATCCT
TGCTGCCCTAAATACAAGTGTGAGTGCCCGGCCACATGCAGCTCCCCACCTGTTTGTGGA
AATGGAAGACCACCCATCCAATATTTCGACCCAACTTCTCAGTGCTGTCCCCTATATGAA
TGCCCTGACGAGACAACCACCGCACCACCAAGGAGCCCAACTACTCCGGAGCTATGCAAG
GGTGTGACCTGTGAAATAGTGACTTGTGCAGAGGGTGACGAAAAGGTGGCAGTTGCCTCA
TCTGATCCATGCTGTTCCCGCTATGTGTGTGTTACGCCAACAACCCCAACTCCAATCAGA
CCTACAACACCGGAATCTTGCAAGGATGTGAAATGTGAAGTGGTGACATGCTACTTTGGA
GAAATAAAGAAAGAGGTTCCTTCAGCAGATCCCTGTTGTCCACGTTATGAGTGTTATCCA
GAGCCAACTACAACCACAGCTACAACTCCGGAACTATGCAAATATGTGACATGCCCAACA
GTGACTTGTGCCAAGGGAGAAACTCAGATGGAAGCTCCTTCAGCAGACCCCTGCTGCCCA
CGTTTTATTTGTGTTCCAGAAACTTCACCCACACCAAAGGAGCCATGCAGTCATGTGACA
TGTACAGACGTGACATGCGCCGCTGGAGAAACCAGAACAGAAACTCCTTCTGGAGATCCC
TGCTGCCAACGTTTTGTGTGTGTTCCACTGCCAACCACAACTCAACCACCGGAGCCCTGC
CAGTATGTGACATGTGCTGATGTGACATGTGCAGATGGAGAAACTAAGATGGAAGTTCCG
AAAATTGATCCCTGCTGCGCACGTTATGTGTGTGTTCCACAAGCAACTACAACCCCACAA
CCGGAAATATGCAAGGGTGTAACATGTGATGTGAAGACATGTTCAGAGGGGGAAGACCTG
GTGGCAATTACTTCAACAGATCCTTGCTGTTCCCATTATGTGTGCGTTAAAATCGTTACT
CTGCCTCCATTCACCTCTACACCAATAATTACACAGGGGGTGTGTAGCGATGTCAAGTGT
GAGGTGGTGAAATGTACGAAGGACGGAGAGACTACGGTGGAAATTCCTTCAGCAGATCCT
TGTTGTCCACGCCACGTCTGTGAATGCAATAACATATGCACCCCTCCTCCTATCTGCATT
GATGGAAGACCACCAGTAAAGATATTTGACCCTGCTACTCAGTGCTGTCCTCAGTATGAA
TGCAGGCATGAGACAACTTCAACACCACCTCCAGAGGACATCTGCAAAGGTGTGACTTGC
AATACTCCAACATGTGTGAGGGAGGGTGAACAAATGGTTGAAGTTCCTTCCTCAGATCGC
TGCTGTCAAAAATATGTGTGCGAATGCTATCAGACATGCAGCCCTCCTATTACCTGCATG
GATGGAAAACCACCAATGGAAATCTTTGACCCTAATACTCAATGCTGTCCAACCTATGAA
TGCTTACCAACAATTACCACCTTACCACCCGAACCACCAAAAACCACACCAGAGGAAATA
TGCAAGGATATCCAATGCCCAGTGATAACTTGCACAAAGAATGGTTCCACTCTTGTGTCT
GTCAAAGGGAACAATCCTTGCTGTAACATAGAATATTGTGACTGCATTGACCCATGCCCA
CCAGCTCTGGTTTGTAAATCAGGCAAGCCAACCATGGAGATTGATCCAGAAAAAGATTGC
TGCCCCAAATACACCTGTATTCCTGATGCAATTCCAGGAACACCTACTACTCCATCTGTA
ACAGAGCCGGAACTCACAACAACACCCAGACCACCAATCTTCACCACTCCGCCTGAATGT
GCCAACGTCAAGTGTTACAAAGACGTTACCTGTCTATACAATGAGATAGAGATTCAGGAA
ACGAATCCACTTGATCCTTGTTGTCCAAGATATCGTTGTGAGCCCAAGACAACCCCAAGC
CCACCGGAAATCACTACGACACCCAGACCACCAATCACCACCACTCCGCCTGAATGTGCT
GATGTCAAGTGTCACAAAGAGGTTACCTGTCAATATAACGAGATAGAGAGGGAGCAACCA
AATCCACTGGATCCTTGTTGTCCATTATATTGGTGTGAGCCCGAGACAACCCCAAGCCCA
CCGGAATTCACTACAACACTTAGACCACCAATCACCACCACTCCGGCTGAATGTTTCGGC
ATCACATGTTACAAAGATACTACTTGTCGATATAACGAGATAGAGATTGAGGAACCAAAT
CCATTTGATCCTTGTTGTCCAAGATATCGCTGTGAGCCCAAGACAACCCCAAGCACAACA
ATGCCGATCACCACCAAGCAAGAATGTGATAATGTCAAGTGTTACAAAGATGTCGTCTGT
GGACCTAATGACAATGAGGTTGAGGAGCCAAATCCATTTGATCCTTGCTGTCCCAAGTAT
CGCTGTGAACCAAAATCTACAACCCCATCTACAATAACAACCAGCACCATTATCACCACC
ACCAAGATTGATTGCAGTAAAAGGATATGTGTCAAAATAAGCTGTCGTCTTCATGAAAAA
GTTATTGAGAAACCAAATCCGGATGACTCCTGCTGTCCCATTCAAATGTGTGAGTGCCAA
TGCAAGACAGTGCCAACGTGCAGAAGTGATGAGAGACTGGTGACCGTGAGTCAGACTGGC
CAGTGTTGTCCAAAGCTTAAATGTGAACGTAAGAAGGATGAATGCCATCCAGTGACTAAA
TCTGTGCTTCTGACATCAGGCCAATGCAAAGCTACAGTGGAACTGGATGTGTGCAGTGGA
TACTGTCACTCCAAGACAGAATATTCCAGCTTTTGGCAGCCCGTAACTCATTGTCGATGC
TGCTCTATAAAAACCGATCGCTTCAGGACCTTCCAACTGCCTTGTGCTGACGGCACTCAC
ACAAGTCTGATTTTAAAGAGTGCAGTAACTTGTGGCTGCAACACTTGCTCAGGGCCCTTT
GAACCTGGAAGTGGCAGCGGCAGCGGCAGTGGCAGCGGCAGTGGTGAAGAAGGCTCAAGC
GAAGGAAGTACCTTCCATACATGGCCAGATTTCATCAGAAGCTAA
Protein sequence predicted based on nucleotide sequence
MRVLPFLALTVACVFSTGSGDNGDCFFYDQVYKDGDTIKYDCQICECTNGRITDCARDAN CIFKKVATPEQNTDAIVYDDDDPNIIESSESKGNVRAKRAASGGHKKNPSAYETNNKFKP NQSNRNDNDDSSKEHKDSNKGKGQKINNVLSKDSSEENASKEKWSHEKNDKMKTNERKGK DHNDSSKESKEKNHEKRQNNKNGWLKDSSEENDSASKEKKDHDKNNKVRTNQNKGNDHDH SSKESKENSHEKGEKSKKRWQNGSAEEKDDSSKEKKDHEKNDKVKPKHNKGNGHDDSSKE SKENSHEKREKNKNRWQKGSAEEKDDGSKEKKDKTLEEMDTNACIFNKESIADGETKDLS CSTCTCNKGKLTCVKNLNCPGVCSVTGYQMVRTFDGSLYQSPGTCNYILVKTSDFTISLS NKACSDQNPDAVCIDTVDIYVPNIASIKVLQDGSVLSAGQKVDLPFFLHESITVRRSSST FLDVVTSMFSLQYDIEGNRLYVIIETSYKDQTSGLCGTYNDNRFDDFRSSSDITETVSSL FSKSWKLQSQCSEHFITPVSPAQRVTADLSCTNSLQDSIFADCNLIIDIHNFQASCSNSI YYGKNVGFCSAMADYAYRCALAGVYVPVSSAFPDCFVSCEGGMVPITENLFTQDDCAQHS SEILKMAVKLPLSEGCVCPNHLYFDSTRNECVDSEECPCYSSNHAYRIGENITFSNGQSC ACERIMACTDNPDIPIDEVPTCPDDEVFSDCLMGSGKSCEPSCQNMAVIDQVCPLECEAG CICKYGKLRSNDGTCVPLQECPCVHGEDVYNPGETLAQDCNTCTCKDGKFTCTNNACNVV CNAYAGGQFILFDNIWKTFKTGECQIILVESQDGQVPKFRVVMQNTKTENLGGALATKKI FIRFGGAYVELSDADPVLVQDLGSTTQVRLYRSGFYIVAHFLEGLAVYYDQHLDVIIQLQ PHLQGKVQGMCGDADQTTTAELAISNMAQYGAQFIIGKCTSSDEPSPPPSDNNKHYTEKR CSLLFSPDFAECHTIVPVQPYYTACVEETEACREGESCLCFCTALSAYARACCRKGINVD WRNPDTCPSPCEYYNRDSGEGPYRLVMVNGQTLMADYDTQSVSLITKDTSGDVKVSFMVT PSLFVDPLYGRKLISLESALHRNFFIVQNADGTLGLRKWQPSASFRIQATFILREGRWIN GYSTLESYSSRGQYLSFNNTNKVLVMTRVKSTNMLAMNFKLTEETFGLPSFSICTWKYRS CGPACIPTCQDPLGEKCTLTLKVEGCFPICAPGMVFDEVTHRCVNKDDCVSIPTVSPTPV PPTPTKEPCKDVTCVEMNCKVGETRVEIKTTDPCCPRYQCEAIPTTPAPPTTPNPCKDVN CEIPTCNSDEQLVQVKSTDPCCPKYKCECPATCSSPPVCGNGRPPIQYFDPTSQCCPLYE CPDETTTAPPRSPTTPELCKGVTCEIVTCAEGDEKVAVASSDPCCSRYVCVTPTTPTPIR PTTPESCKDVKCEVVTCYFGEIKKEVPSADPCCPRYECYPEPTTTTATTPELCKYVTCPT VTCAKGETQMEAPSADPCCPRFICVPETSPTPKEPCSHVTCTDVTCAAGETRTETPSGDP CCQRFVCVPLPTTTQPPEPCQYVTCADVTCADGETKMEVPKIDPCCARYVCVPQATTTPQ PEICKGVTCDVKTCSEGEDLVAITSTDPCCSHYVCVKIVTLPPFTSTPIITQGVCSDVKC EVVKCTKDGETTVEIPSADPCCPRHVCECNNICTPPPICIDGRPPVKIFDPATQCCPQYE CRHETTSTPPPEDICKGVTCNTPTCVREGEQMVEVPSSDRCCQKYVCECYQTCSPPITCM DGKPPMEIFDPNTQCCPTYECLPTITTLPPEPPKTTPEEICKDIQCPVITCTKNGSTLVS VKGNNPCCNIEYCDCIDPCPPALVCKSGKPTMEIDPEKDCCPKYTCIPDAIPGTPTTPSV TEPELTTTPRPPIFTTPPECANVKCYKDVTCLYNEIEIQETNPLDPCCPRYRCEPKTTPS PPEITTTPRPPITTTPPECADVKCHKEVTCQYNEIEREQPNPLDPCCPLYWCEPETTPSP PEFTTTLRPPITTTPAECFGITCYKDTTCRYNEIEIEEPNPFDPCCPRYRCEPKTTPSTT MPITTKQECDNVKCYKDVVCGPNDNEVEEPNPFDPCCPKYRCEPKSTTPSTITTSTIITT TKIDCSKRICVKISCRLHEKVIEKPNPDDSCCPIQMCECQCKTVPTCRSDERLVTVSQTG QCCPKLKCERKKDECHPVTKSVLLTSGQCKATVELDVCSGYCHSKTEYSSFWQPVTHCRC CSIKTDRFRTFQLPCADGTHTSLILKSAVTCGCNTCSGPFEPGSGSGSGSGSGSGEEGSS EGSTFHTWPDFIRS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
