klhl18 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATATGGGGAGCAGCGGTA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGTGGACGAATTGGAGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708202
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1719nts
Open reading frame: 1 to 1719

ATGGTGGACGAATTGGAGGAGCTGGAAGACCTGGTTCATGTGACGGTCGGGGACCTTCCC
AGCCGGGGTTACGGGGTGATGGAGGAGATTCGGCGGCAGGGAAAGCTGTGTGATGTCACA
CTCAGGGTGGGGGAACATAAATTCAGTGCCCATCGGATTGTGCTGGCAGCTTCCATCCCG
TATTTCCATGCCATGTTCACCAACGACATGATGGAGTGCAAGCAGGACGAGATAGTCATG
CAGGGAATGGACCCAAGTGCCCTGGAAGCTCTGATTAACTTTGCCTATAACGGGCACCTT
GCCATTGATCAGCAGAACGTTCAGTCCTTACTGATGGGCGCCAGCTTCCTGCAGCTTCAG
AACATCAAGGACTCCTGCTGCTCCTTCCTAAAGGAGAGACTGCACCCAAAGAACTGCTTA
GGGGTCCGGCAGTTTGCAGAGACCATGATGTGTGCGGTGCTGTATGATTCTTCCAATCGC
TTCATCCACGAGCACTTTGTGGAGGTCTCCATGTCTGAAGAGTTCCTGGCGTTGGCCTTT
GAGGAGGTGCTGGAGTTGGTCGCTAGGGATGAGCTGAATGTGAAGGCCGAGGAGCAGGTG
TTTGAGGCAGCTCTGGCCTGGGTGCGGTATGACCGGGAGAACAGAGAAGTTTTTGTTCCT
GAGCTTCTGTCCAAAATCCGCCTGCCCCTCTGCCGACCGCAGTTCCTCACGGACAGAGTT
CAGCAAGATGACTTGGTTCGCTGCTATCATAAGTGCAGGGATCTAGTGGATGAAGCAAAG
GATTATCACCTGATGCCAGAGCGACGGCCCCACCTCCTTGCTTTTAAAACCCGCCCTCGC
TGCTGCACCTCTATTGCGGGGCTGATCTATGCAGTGGGGGGGCTGAATTCAGCAGGCGAC
TCCTTGAACGTGGTGGAAGTCTTTGACCCGATTGCCAACCGCTGGGAGAAGTGTCAGCCA
ATGACGACTGCCCGGAGCCGCGTGGGTGTGGCTGTTGTGAATGGACTTCTGTACGCCATT
GGTGGATACGACGGCCAGTCAAGGTTGAGCACTGTGGAGGTTTATAACCCGGAGACTGAC
ACTTGGACCAAAGTAGGAAGCATGAACAGCAAGAGAAGCGCTATGGGCAGTGTGGTGTTG
GATGGTCAGATTTACGTGTGCGGAGGATATGACGGCAACTGCTCTCTGAACTCCGTGGAG
GCGTATTCACCCGAAACAAACAAATGGACGGTAGTGACGCCAATGAGCTCCAATCGCAGT
GCAGCTGGTGTGACGGTATTTGAAGGTCGAATATACGTTTCTGGGGGGCACGATGGGCTG
CAGATCTTTAATACGGTGGAATATTACAACCATCACACGGGGACGTGGCACCCAGTATCG
AGCATGTTGAACAAGCGCTGTCGTCATGGTGCCGCCTCATTGGGCAGTAAAATGTATATC
TGTGGCGGTTACGAGGGCTCGGCGTTCCTCAGCGTGGCAGAAGTGTACAACTCCATGGCG
GATCAGTGGTACCTCATCACTAACATGAGCACCCGGAGGAGCCGCGTTTCACTCGTAGCC
AACTGTGGGCGTCTCTATGCTGTGGGAGGCTACGACGGCCAGTCCAACTTGAACTCAGTA
GAAATGTATGACCCGGAGACCAACCGCTGGACTTTCATGGCCCCCATGGTGTGTCACGAA
GGAGGTGTGGGGGTGGGCTGTATACCGCTGCTCCCCATATAG

Protein sequence predicted based on nucleotide sequence

MVDELEELEDLVHVTVGDLPSRGYGVMEEIRRQGKLCDVTLRVGEHKFSAHRIVLAASIP
YFHAMFTNDMMECKQDEIVMQGMDPSALEALINFAYNGHLAIDQQNVQSLLMGASFLQLQ
NIKDSCCSFLKERLHPKNCLGVRQFAETMMCAVLYDSSNRFIHEHFVEVSMSEEFLALAF
EEVLELVARDELNVKAEEQVFEAALAWVRYDRENREVFVPELLSKIRLPLCRPQFLTDRV
QQDDLVRCYHKCRDLVDEAKDYHLMPERRPHLLAFKTRPRCCTSIAGLIYAVGGLNSAGD
SLNVVEVFDPIANRWEKCQPMTTARSRVGVAVVNGLLYAIGGYDGQSRLSTVEVYNPETD
TWTKVGSMNSKRSAMGSVVLDGQIYVCGGYDGNCSLNSVEAYSPETNKWTVVTPMSSNRS
AAGVTVFEGRIYVSGGHDGLQIFNTVEYYNHHTGTWHPVSSMLNKRCRHGAASLGSKMYI
CGGYEGSAFLSVAEVYNSMADQWYLITNMSTRRSRVSLVANCGRLYAVGGYDGQSNLNSV
EMYDPETNRWTFMAPMVCHEGGVGVGCIPLLPI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable