sytl1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAACGTGAAGTTAAATTTTGTCT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGATCCCACGTGGGGAGACAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708210
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1569nts
Open reading frame: 1 to 1569

ATGATCCCACGTGGGGAGACAATGGATCCGGGGGAGAGTGGAGAGCTTCTAGACCTCAGT
TTCTTAACTGGAGAAGAGCAACTAATGATAGTACAGGTGCTAGAGCGAGACACAGAGCTG
AAGGCCAAAGAAGATCAGCGCATAAACAAGCTGCGATCAACTCTGTCAGACAAGAAAGCA
TTCAAACGTCTGTCAGGGGAATGGTTCTGTGATGTGAGGGCTACACGTCCTTGGGGACAG
ACAGGTGGTATTGACATTGTGCAGGCATCAATTAGGAGGAAAAAAAAAGTCAGAGGTGAA
TGTGATGTAAACTGGGGTCAAGATGAGAATGGAGAGAGTGACGGACCAGAAGAAACAGAA
ACGTTGGCACCTGTGAGAAAATTATCCACGGAATCCCTTCCAGATCGGCTCTCCAGTGAT
GCACAGGATTGGAGCAGCTCAAAAGAGGATGAGGGGGGAGACACAACAGATGGGAAGAAC
AGTATGGAAGAAATAAACTTATCTCCAGTAAATGGCACAGTTTCAAGTGAAGATACTAAT
GGGGAAAGCCTGGAAGAAAAAGAACACCGGAACCTCAACCACAGCCCATCAATTCGACAG
CTGGCAAATAATTCTCAAATTTCTGGCAGTATGATGAGCCTGTTTAGCACAGGGGAAGTG
CGATCAGTTGACGTTCGTGGCTGTGTCCAGTTCTCTCTTCAATACGATCCCTCCAAGAAG
GAGCTGAATGTCTTAATTATTCAATGCAGGGACCTGACACCCGCACAGAGCAAAACCTCC
AATCCGTATATAAAGTGTTATTTGCTCCCTGATAAAACTGCTCAGGGGAAGAGGAAATCA
AAGTTGAAAAAGAAGACTCTGGAACCGTTCTTTAATGAGACCCTAAAATACAAGGTGGAA
TGGTCAGAGTTGCAAAGCAGAGTCCTCAATCTGTCTGTGTGGCACAGGGGGTCACTGGGA
AGAAACCTTTTTCTTGGTGAGGTGGAAGTGGATCTTGTGTCCTGGGACTGGAGCAAAACT
CAGCCAGCCTGGTACAATCTCCAGCCAAGGACTCCCCTATCTCCAGAATTCCTTTGCAGT
CGAGGTCGGCTTAGAGTTGCTGTGAAATATGTTCCACAAGGGGCAGAAGGCTTAGGGCTT
CCTCCCACTGGTGAACTCCATGTTTGGATTAAGGAGGCACAGCAACTTGTACCTCACAAG
GCTGGAAATGTCAGCACTTTTGTAAAGTGCTTTGTGCTTCCCGATTCCAGTCCATCCAAC
ATACAGCGCACACGTGTGATCCCACATAGCTTGCATCCAGTGTTTAACCACACCATGGTG
TATGATGGCTTCAGTGGAGAAGACCTCAAAGAAGCCTGTGTTGAATGCATCATTTGGGAC
CAGGGTAAAATAGGAAGTCGACCTCTTGGGGGCATTCGCCTCAGCACCGGAAAAGGAAGC
AGCTATGAAAACAGTGTCAACTGGATGGATTCCACAGAAGAGGAGAAGGCATTCTGGAAA
TCAGTTATAAATAATCCTGCAGAGTGGGCAGAGATTGTGCTGCCACTAAGACAAAATTTA
ACTTCACGTTGA

Protein sequence predicted based on nucleotide sequence

MIPRGETMDPGESGELLDLSFLTGEEQLMIVQVLERDTELKAKEDQRINKLRSTLSDKKA
FKRLSGEWFCDVRATRPWGQTGGIDIVQASIRRKKKVRGECDVNWGQDENGESDGPEETE
TLAPVRKLSTESLPDRLSSDAQDWSSSKEDEGGDTTDGKNSMEEINLSPVNGTVSSEDTN
GESLEEKEHRNLNHSPSIRQLANNSQISGSMMSLFSTGEVRSVDVRGCVQFSLQYDPSKK
ELNVLIIQCRDLTPAQSKTSNPYIKCYLLPDKTAQGKRKSKLKKKTLEPFFNETLKYKVE
WSELQSRVLNLSVWHRGSLGRNLFLGEVEVDLVSWDWSKTQPAWYNLQPRTPLSPEFLCS
RGRLRVAVKYVPQGAEGLGLPPTGELHVWIKEAQQLVPHKAGNVSTFVKCFVLPDSSPSN
IQRTRVIPHSLHPVFNHTMVYDGFSGEDLKEACVECIIWDQGKIGSRPLGGIRLSTGKGS
SYENSVNWMDSTEEEKAFWKSVINNPAEWAEIVLPLRQNLTSR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable