eed-b (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACCGGAGCCTGTCCCATC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCCGGCCAAGAAACAGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708221
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1278nts
Open reading frame: 1 to 1278

ATGCCGGCCAAGAAACAGAAGCTGAGCAGCGATGAGAACAGTAACCCGGAGTTGTCTGGG
GATGAGAACGATGATAGTGTCAGCATAGAGAGTGGTACAAACACAGAGCGCCCAGACACC
CCGACAAATGCAGCAAATGCCCCTGGCAGAAAAGGCTGGGGCAAAGGAAAATGGAAGTCA
AAGAAATGCAAATACTCCTTCAAATGTGTAAATAGCCTTAAGGAAGATCACAATCAGCCA
CTATTTGGGGTTCAGTTTAACTGGCACAGTAAGGAAGGAGACCCGTTGGTTTTTGCCACT
GTTGGGAGTAACAGAGTAACATTATATGAATGCCACCCGCAGGGGGATATCCGACTGCTG
CAGTCCTATGTCGATGCCGATGCAGATGAGAACTTTTATACTTGTGCTTGGACGTATGAC
AGCAATACAAGTCACCCGCTTCTAGCAGTGGCTGGTTCTAGAGGAATCATTAGGATAATT
AATCCTATTACTATGCAGTGTATCAAGCACTATGTGGGTCACGGCAATGCTATCAATGAG
CTTAAATTTCACCCACGGGATCCAAACCTCCTCTTATCAGTAAGCAAAGATCACGCTCTA
AGACTTTGGAACATACAGACAGATACTCTTGTTGCCATTTTTGGTGGAGTGGAAGGTCAC
AGAGATGAAGTGTTAAGTGCGGACTATGATTTGCTTGGTGAGAAGATCATGTCATGTGGT
ATGGACCATTCCCTCAAACTCTGGAGAATTAACTCTTTAAGAATGAAGACCGCCATCAAA
GAGTCTTATGATTACAATCCCAATAAAACAAACAGGCCATTTGTCTCACAGAAGGTCCAT
TTTCCTGATTTTTCCACAAGGGACATTCACAGAAACTATGTAGATTGTGTCCGGTGGTTA
GGAGACCTTATTTTGTCTAAATCTTGTGAAAATGCGATTGTGTGCTGGAAACCTGGCAAA
ATGGAAGATGATATCGAGAAGATCAAAGCCAGCGAGTCCAATGTCACCATTCTCGGCAGA
TTTGATTACAGTCAGTGTGATATTTGGTATATGAGGTTTTCAATGGACTTCTGGCAAAAG
ATGCTGGCCTTGGGTAACCAAGTAGGAAAGCTCTACGTCTGGGACTTGGAAGTAGAAGAT
CCACATAAGGCCAAGTGTACAACACTCACGTACCCCAAATGTGCGTCAGCAATCCGACAG
ACGAGTTTCAGCAGAGACAGCAGTGTCCTCATAGCCGTGTGCGACGACTCTACCATTTGG
CGATGGGACAGGCTCCGGTGA

Protein sequence predicted based on nucleotide sequence

MPAKKQKLSSDENSNPELSGDENDDSVSIESGTNTERPDTPTNAANAPGRKGWGKGKWKS
KKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHPQGDIRLL
QSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINE
LKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCG
MDHSLKLWRINSLRMKTAIKESYDYNPNKTNRPFVSQKVHFPDFSTRDIHRNYVDCVRWL
GDLILSKSCENAIVCWKPGKMEDDIEKIKASESNVTILGRFDYSQCDIWYMRFSMDFWQK
MLALGNQVGKLYVWDLEVEDPHKAKCTTLTYPKCASAIRQTSFSRDSSVLIAVCDDSTIW
RWDRLR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable