pfkfb4 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAAGGTGATTTGGTACAGTCTGTAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTGATTCCCCCCT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708480
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1410nts
Open reading frame: 1 to 1410

ATGGCTGATTCCCCCCTGGAGCTGACCCAAAACCCACTAAAAAAAATTTGGGTGCCTTTT
AACAATGGACATCCGGTGCAGCACAGTGCCCAGAGGAAAGTATGTATGACCAACTGCCCG
ACCCTCATTGTTATGGTCGGACTCCCTGCCAGAGGCAAGACTTACATCTCCAAGAAACTC
ACACGTTATCTCAACTGGATTGGGGTGCCAACCAAAGAATTCAATGTTGGCCAGTACAGA
CGGGACCTGGTGCAAACCTTCAAATCCTTTGAATTCTTCCTTCCAGACAACGAGGAGGGA
CAGAAAATCCGAAAACAATGTGCCTTACTGGCCTTGAATGATGTTAGGCGCTATCTGGGA
GAAGAAGGGGGGCATGTGGCAGTTTTTGATGCAACAAATACAACAAGAGAAAGGCGAGAG
ACCATCCTTAAATTTGCAGATCAGAATGGTTTTAAAACTTTCTTTGTGGAATCTGAGTGT
GTGGACCCTGAAGTTATTGCTCAGAATATAGTGCAAGTGAAGTTAGGGAGCCCAGATTAT
CTGCACTGTAGCAGCGAGGAGGCAACCGAAGACTTCATGAAAAGAATTGATAGCTATAAG
AACTTCTATGAGACCCTGGATGAGAAATTGGACAAGGACCTGTCCTATATTAAGATCATG
GATGTAGGCTGCAGATATCTTGTTAATCGAGTTATGGATCACATCCAGAGTCGCATTGTC
TACTACCTCATGAATATTCACATCACTCCACGCTCCATCTACTTGTGCCGACATGGGGAG
AGTGAGCTCAATCTCAAGGGGCACATTGGTGGCGATTCTGGTCTCTCCAACCGAGGGAAA
GAGTTTGCTAAATGTTTGGGCAGTTACATCCGTGAACAAAACATCCATGACCTTAAAGTG
TGGACCAGCCAAATGAAGAGAACAATCCAAACTGCAGAGGCCCTGACTGTGCCCTATGAA
CAGTGGAAAGCCTTAAATGAAATTGATGCTGGGGTGTGCGAGGAAATGCGCTATGAGGAA
ATCCAGGAATCCTACCCGTTGGAATTCGCTCTGCGTGACCAGGATAAATACAGATATCGT
TATCCGAAAGGAGAGTCCTATGAAGATCTTGTACAGCGCTTGGAGCCCGTCATTATGGAA
CTGGAGCGTCAGGAGAATGTGCTGGTCATTTGCCATCAAGCTGTCATGCGCTGCCTGCTG
GCCTACTTCCTTGATAAAAGTGCAGAGGAGCTGCCTTACCTCAAATGCCCCTTGCATACA
GTGATGAAACTCACCCCTGTAGCTTATGGGTGTAAAGTGGAAGCTATCCGTTTAAATGTG
GAAGCGGTTAATACTCATAGGAATAAGCCAGAGAATGTTGATGTGGACCGCTCTAGTGAA
GATGCTTTACAGACTGTACCAAATCACCTTTAA

Protein sequence predicted based on nucleotide sequence

MADSPLELTQNPLKKIWVPFNNGHPVQHSAQRKVCMTNCPTLIVMVGLPARGKTYISKKL
TRYLNWIGVPTKEFNVGQYRRDLVQTFKSFEFFLPDNEEGQKIRKQCALLALNDVRRYLG
EEGGHVAVFDATNTTRERRETILKFADQNGFKTFFVESECVDPEVIAQNIVQVKLGSPDY
LHCSSEEATEDFMKRIDSYKNFYETLDEKLDKDLSYIKIMDVGCRYLVNRVMDHIQSRIV
YYLMNIHITPRSIYLCRHGESELNLKGHIGGDSGLSNRGKEFAKCLGSYIREQNIHDLKV
WTSQMKRTIQTAEALTVPYEQWKALNEIDAGVCEEMRYEEIQESYPLEFALRDQDKYRYR
YPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSAEELPYLKCPLHT
VMKLTPVAYGCKVEAIRLNVEAVNTHRNKPENVDVDRSSEDALQTVPNHL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable