fubp3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACTGCTCCTGGTTGTGAGAATGAGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCAGAGCTGGTGCAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708615
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1638nts
Open reading frame: 1 to 1638

ATGGCAGAGCTGGTGCAAGGACAGAGCGCGGCTCCTGTAGGGATAAAGGCCGAGGGATTC
GCCGACGCGCTGCACCGGGTCCGACAGATAGCTGCTAAAATTGACGGAATCCCCCACTTG
AATAATTCCGGACCTTTAGTGGACGCATCTTTGTATGGTTATGGAGTCCAAAAGAGACCC
ATGGATGATCCTGTTGGTAACCAGTTAGGGGCCCTCGTACATCAAAGGGCTGTGATGACC
GAGGAATATAATGTGCCTGACAAGATGGTGGGATTCATTATTGGACGAGGTGGGGAGCAG
ATCTCACGGATCCAAGCAGAATCTGGTTGCAAGATACAGATAGCTCCAGACAGCGGAGGT
ATGCCAGAGAGACCTTGTGTCCTGACCGGAACACCGGAAAGTATAGAACAGGCAAAACGA
CTCCTGGGCCAGATTGTGGATCGTTGTCGGAATGGACCAGGGTTCCACAATGACATGGAC
GGCAGCAGCACAGTCCAGGAGATCCTCATCCCGGCTTCAAAAGTGGGCCTAGTCATCGGC
AAAGGCGGGGAAACCATCAAACAGCTGCAGGAGAGGACTGGTGTTAAAATGATCATGATC
CAAGATGGTCCTTTACCAACTGGTGCAGACAAGCCTCTGAGGATAACTGGTGACCCTTTC
AAAGTGCAGCAAGCAAGAGATCTGGTCCTGGAGATCATCCGAGAAAAAGACCAGGCTGAC
TTCAGGGGCATCCGAAGTGATTTCAGCTCAAGGATTGGTGGGGGTAGCGTAGAGGTGTCT
GTACCTAGGTTTGCTGTGGGAATCATAATAGGAAGAAATGGTGAAATGATAAAAAAGATC
CAGAATGATGCTGGAGTGAGGATTCAGTTTAAGCCAGATGATGGGCTCAGCCCTGAGCGA
GTGGCACAGGTAATGGGCCTCCCTGACCGGTGTCAGCATGCTGCTCATATCATCAATGAA
CTTATTCTTACAGCACAGGAACGTGATGGCTTTGGAGGTCTCTCCTTGGCACGGGGAAGG
GGACGGAGCCGTGGTGATTGGAACCTCAGTACCCCAGGAAGCATGCAAGAGATCAACTAC
ACTGTGCCGGCCGACAAATGTGGTCTCGTGATTGGCAAAGGTGGTGAAAATATCAAAAAT
ATAAACCAGCAGTCAGGGGCTCATGTGGAGCTACAGAGAAACCCCCCTCCCAACACAGAC
CCAGCTGTCAGGATATTTACCATCCGGGGTGTCCCCCAGCAGATTGAGCTGGCCAGGCAT
CTCATCGATGAGAAAGTTGGGGGTACTACAATAGGTGGCCCCAGCAGCTTTGGTCAGAGT
GCTTTCAGCCAGGCACATGCTACACCTCATCAAAATGGTCCTCAAGCTTTTGTAACACAG
GGTTGGGGTAGCACTTACCAGGCATGGCAGCAAACTGCACAGCAGATCCAAGGTCCGCAA
AGCCAGCAGCAAAATGCACAGCCCGATTACAGCAAGGCCTGGGAAGAGTATTACAAGAAG
CAGAACCAGGCTGCCAGCGCCACTTCCCAGGCAAGTTCACAACCAGACTATACGATGGCA
TGGGCTGAGTATTATAGGCAACAGGCCGCTTATTATGGGCAGTCACTGGGGCAGACTCAT
TCTCACAACCAGGAGCAGTAG

Protein sequence predicted based on nucleotide sequence

MAELVQGQSAAPVGIKAEGFADALHRVRQIAAKIDGIPHLNNSGPLVDASLYGYGVQKRP
MDDPVGNQLGALVHQRAVMTEEYNVPDKMVGFIIGRGGEQISRIQAESGCKIQIAPDSGG
MPERPCVLTGTPESIEQAKRLLGQIVDRCRNGPGFHNDMDGSSTVQEILIPASKVGLVIG
KGGETIKQLQERTGVKMIMIQDGPLPTGADKPLRITGDPFKVQQARDLVLEIIREKDQAD
FRGIRSDFSSRIGGGSVEVSVPRFAVGIIIGRNGEMIKKIQNDAGVRIQFKPDDGLSPER
VAQVMGLPDRCQHAAHIINELILTAQERDGFGGLSLARGRGRSRGDWNLSTPGSMQEINY
TVPADKCGLVIGKGGENIKNINQQSGAHVELQRNPPPNTDPAVRIFTIRGVPQQIELARH
LIDEKVGGTTIGGPSSFGQSAFSQAHATPHQNGPQAFVTQGWGSTYQAWQQTAQQIQGPQ
SQQQNAQPDYSKAWEEYYKKQNQAASATSQASSQPDYTMAWAEYYRQQAAYYGQSLGQTH
SHNQEQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable