sall1-b (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  sall1-b
Gene Name:  spalt-like transcription factor 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGTTCTTACCTTAAGGTTGCCTTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAAATCTGAACCAGAAGAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708817
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3447nts
Open reading frame: 1 to 3447

ATGAAATCTGAACCAGAAGAAATAAAACTAAAGTTTAATCATGAAAGAAGAACAGAGAGG
GGTCAAATCCATCGGACGCCCAAGAACAAGGAGGCGCATGTCTGTAGCAGATGTTGTGCA
GAGTTCTTTGAACTATCAGATTTGTTGCAACACAAGAAGAACTGCACTAAAAATCAATTG
GTTCTAATTGTGAATGAAAATCCACCACCTCCTGCTGAAGCCAGCTCTCCTAGTCTCCCC
TCAGATAATCCTGATGAGCACATGAATGACACAGTTAATAACTCAGATCAAGCAGACTGC
AGTGACCTTTCAGAGCATGGTGAGCACGGCAAGCCTGACAAAGAAGAATCCATGGAGGTG
GAGATTTCGGAAACTAACAATAGCAGCAGTGGTTCTAACAAAGTGGACAATGGTGATTCA
AGCAGTAATAATCCCACATTGGGTACCTCAGCTATCACAACTTCGCTACCTCAAGTAAGG
GATCTGACAGCTTTGGGCAACTTCTCTATGATTAACAGCAATGTCATAATTGAGAACCTT
CAGAGCACTAGAGTGGCAGTGGCACAATTCTCCCAAGAAGCCAAAACTAAGGGGGCAGCA
AACAACAAGTTGGCAGTGCCTGCACTCATGGAGCAGCTCTTGGCTTTACAGCAGCAACAG
ATTCACCAGTTGCATCTGATTGAACAAATCCGTCACCAAATATTACTGTTGGCATCACAG
AGTGCAGAGATGCCCACTTCATCTAGTGGCCCTCAAGGGGCTTTAAGGGTATCTGCCACT
CCACTGACTACTCTGAGTTCCCATTTGTCCCAGCAGCTAGCTGCAGCAGCTGGCTTAGCA
CAAAGTCTTGCTAGCCAATCAGCTAACATCAGTGGTATGAAGCAACTACCCCCCATACAG
CTACCTCAGAGCAACCCAGTTAACACAGTTCCATCCATCAGTAGTTCCTCTCCAAATATA
AATTCATTGGCAGCAACAGTTACTCCACCCTCCTCAGAGAGAGTACCTTCAAGCTCTGGT
GGCTCACAGGTCAGTAACACACCCGTTCCTGTAACATCATCACCAGCTTTTGCAATAAGT
AGTTTATTAAGCCCTGCACCCAATCCACTACTACCTCAACCTGCCCCTAATACCACAGCT
TTCCCTAGCTCATTGCCCAGCATAGGAACAACAGCAGAGGATTTAAACTCCCTGAGGAAA
AGTAAGCCACCCAGTGTGACTGCTTTTGAAACTAAGAGTACATCTGATGAGGCATTCTTC
AAACACAAGTGTAGATTCTGTGCTAAGGTTTTTGGCAGTGACAGTGCCTTGCAGATCCAT
CTACGTTCTCACACTGGCGAGAGACCATTTAAATGCAACATATGTGGAAATCGGTTTTCC
ACCAAAGGGAATCTTAAAGTCCATTTTCAGCGTCATAAAGAAAAATATCCCCATATTCAG
ATGAATCCATATCCAGTACCAGAACATTTAGACAATATCCCTACAGGCACTGGTATACCA
TATGGGATGTCCATCCCACCAGAAAAGCCTGTAACTAACTGGCTGGACACTAAACCAGTT
TTACCTACCTTAACAACATCAGTGGGTATGCCTCTCCCACCAACAATTCCCACCTTGTCT
CCATTTATAAAAACTGAAGAGCCACAGGCTTTATCTATTAGTCATCCCACTGCCAGCCCA
CCTGACTCAGTAAAAAGTGAGACAGCTTCAGAGTCACTTTTAAAAAAAACTAGTCATTTT
TCAGATGAGGCTGAGGCTGCCATGCCACCCATTTTAGACAAAGAGGAACACCAGTCTCAA
AATTCAGACTCTCAACAAAATCTTAATACTTCTGCCTGCTCTCCTACAACTGACTCTGGC
ATCTCAACCATGTTCCCAAATCCACTTTTGCCTCTAATGTCTGAGCAATTTAAAGCAAAA
TTTCCATTCGGGGGACTTTTGGATGCAGCACCTGCATCAGAGACTTCAAAACTTCAACAG
CTTGTAGAGAATATTGACAAAAAGTCAAGTGATCCAAATGAGTGTGTTATTTGTCACAGA
GTGCTAAGTTGCCAGAGTGCTCTAAAAATGCATTATAGGACACATACTGGAGAACGACCA
TTTAAATGCAAAGTTTGTGGTCGTGCTTTTACAACTAAAGGTAATTTAAAGACTCATTAC
AGTGTCCATCGTGCCATGCCACCTCTTAGAGTGCAGCATTCATGCCCAATTTGTCAAAAG
AAATTTACAAATGCTGTTGTACTTCAACAGCATATTAGGATGCATATGGGAGGGCAGATT
CCTAATACTCCTGTTGCTGAAAACTACCCTGACTCCATGGGATCAGATACTGGGTCCTTT
GATGAGAAAACTATTGATGATCTAGACAATTTCTCAGATGAAAACATGGAAGATTGTCCA
GACAGCAGTATCCCAGACACCCCTAAATCTATTGATGCATCTCAAGACAGTCTATCTTCT
TCACCACTACCACTTGAAGTTTCAAGTATTACTGCTTTAGAAAATCAAATGAAATTGATC
AATGCTGGACTTGCTGAACAGCTCCAAGCCAGTCTAAAGTCAGCTGAAAATGGGTCTGTT
GAGGGTGATGGTATGACCAATGACTCCTCATCTCTTGGTTGTGACATGGAAAGCCAGAGT
GCTGGAAGCCCTGCTGCCTCTGAATCTACTTACTCCATGCATGCTTTGTCTCCTTTCAAT
AGCACAATTGACTATCTGAAATCACCCAACACAGATGAGAAGCTTCAGCGAGCTGTATCT
CTTGACCCAACCAATGGACTGTCCCCTACACCTGCTAATGGAGGGGCTTTGGACTTGACA
TCTAGTAACACAGATAAAGTGATCAAAGAAGAACCTTTAGGTGTGCTATTCCCTTTCAGA
GATCGGTGTAAATATAAAAACACTATATGTGATATTTGTGGCAAAACCTTTGCATGTCAG
AGTGCCTTGGACATTCATTATAGAAGCCATACCAAAGAAAGACCATTTATTTGCACAGTC
TGCAATCGTGGCTTTTCAACAAAGGGCAATTTGAAACAGCATATGCTCACTCACCAGATG
AGGGATCTACCATCACAACTCTTTGAACCCAGTTCCAGTATGACACCAAACCCAACAATT
CCCTCAGCACCTTCTAACCCTCTGGCAACTATAATAAAGACTGAGTTTAATGGTTTCATG
CATGGCTCTTCTCAGGACATTAAAGAACAGCCTACAACAAACATAGTTTCATCAGGATCT
CTGCCATCTTCTGCCACGTCACCAGTTCTGCTTCCTGCTTTGGCCAGAAGGACTCCTAAG
CAGCATTACTGCCATGCATGTGGGAAATCATTCTCTTCTTCCAGTGCTTTACAAATCCAC
GAGCGGACTCACACTGGTGAAAAACCATTTGCCTGCACTATATGTGGAAGGGCATTCACT
ACAAAAGGCAACCTTAAGGTAAGAACCTGA

Protein sequence predicted based on nucleotide sequence

MKSEPEEIKLKFNHERRTERGQIHRTPKNKEAHVCSRCCAEFFELSDLLQHKKNCTKNQL
VLIVNENPPPPAEASSPSLPSDNPDEHMNDTVNNSDQADCSDLSEHGEHGKPDKEESMEV
EISETNNSSSGSNKVDNGDSSSNNPTLGTSAITTSLPQVRDLTALGNFSMINSNVIIENL
QSTRVAVAQFSQEAKTKGAANNKLAVPALMEQLLALQQQQIHQLHLIEQIRHQILLLASQ
SAEMPTSSSGPQGALRVSATPLTTLSSHLSQQLAAAAGLAQSLASQSANISGMKQLPPIQ
LPQSNPVNTVPSISSSSPNINSLAATVTPPSSERVPSSSGGSQVSNTPVPVTSSPAFAIS
SLLSPAPNPLLPQPAPNTTAFPSSLPSIGTTAEDLNSLRKSKPPSVTAFETKSTSDEAFF
KHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQ
MNPYPVPEHLDNIPTGTGIPYGMSIPPEKPVTNWLDTKPVLPTLTTSVGMPLPPTIPTLS
PFIKTEEPQALSISHPTASPPDSVKSETASESLLKKTSHFSDEAEAAMPPILDKEEHQSQ
NSDSQQNLNTSACSPTTDSGISTMFPNPLLPLMSEQFKAKFPFGGLLDAAPASETSKLQQ
LVENIDKKSSDPNECVICHRVLSCQSALKMHYRTHTGERPFKCKVCGRAFTTKGNLKTHY
SVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPVAENYPDSMGSDTGSF
DEKTIDDLDNFSDENMEDCPDSSIPDTPKSIDASQDSLSSSPLPLEVSSITALENQMKLI
NAGLAEQLQASLKSAENGSVEGDGMTNDSSSLGCDMESQSAGSPAASESTYSMHALSPFN
STIDYLKSPNTDEKLQRAVSLDPTNGLSPTPANGGALDLTSSNTDKVIKEEPLGVLFPFR
DRCKYKNTICDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQM
RDLPSQLFEPSSSMTPNPTIPSAPSNPLATIIKTEFNGFMHGSSQDIKEQPTTNIVSSGS
LPSSATSPVLLPALARRTPKQHYCHACGKSFSSSSALQIHERTHTGEKPFACTICGRAFT
TKGNLKVRT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable