edc3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACATGGAATGTAACGGGATCACAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTGCAGATTGGCTGGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709232
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UNAVAILABLE
No restriction
Special MTA: None
Insert sequence: 1515nts
Open reading frame: 1 to 1515

ATGGCTGCAGATTGGCTGGGAAGTATTGTATCAATAAATTGTGGCAATACCTTGGGAGTT
TATCAAGGCTGTGTTTCTGCTGTGGATCGTATCAATCAAACAATATCTCTTTCTCAGCCG
TTCCACAATGGAGTTAAGTGCTTGGTGCCAGAGGTCACTTTCAGGGCTGGTGACATATCA
GAATTAAAGATTCTTGAAATTCCTTCAGAGAGCCTGAAGTACACAAGCGATCAGCTAAAT
GACCACTCTACAGGCTTTGGCTACCTTCCAACTCGTCAGCAAAATGGTACAGGCAAGCAG
AAGCAGACGGTAGCACATAACTCTGCTCAGAACATTCCTGGCCAAGAGGAGGGAAAGGCT
TCTGAACCATCTAGTACTTCACCACAGCCCTGCTCTAAAAGTTTTGTAGACAGGCACAAT
GAAGCAGTCAACCAGCCCAAAAACTTTCGCCGAAGACACAATTCCTTAGGGTCTTCTAGT
AGCCGCTATCCAAACCAGGTTACACCAAAAAAGAGTGGCACAAAAAATGGACAGCTGAAG
GCGCGTGATGACGAATGCTTTGGAGATGAACTGGAGGAAATTCCTGACACTGACTTTGAC
TTTGAGGGCAACCTTGCTCTGTTTGACAAAGCAGCTGTCTTTGAAGAGATAGATACACAT
GAAAGGAGGGGAGGAGGAGGAACAAGGTCGCGGGGGACCCCAAATGAGAGGCCTCCAACT
TACAGGCATGATGAGAACATCCTGGAATCAGAACCAATAGTATATAGGAAGATTGTGGTT
CCTCAGTCTGGAGGACAGGAATACTGCACAGATTCGGGACTTGTGGTACCCAGTATATCT
TATGTCTTGCACAAGAAGCTACTGTGTGTGGCTGAGAAACATGGCCTTTCAGTGGAGAGG
AGACTGGAAATGTCTGGTGTCTGTGCTAGCCAAATGGCACTTACATTGCTTGGAGGGCCC
AACAGACTGAACCCTAAGAATGTTCATCAGCGCCCTACTGTGGCCCTACTCTGTGGCCCC
CATGTGAAGGGAGCCCAAGGGATCAGCTGTGGCCGGCACTTAGCAAACTATGATGTTGAC
ATTATCCTTTTCCTGCCTAATTTTGTGAAAATGCTGGAGCCAGTTACCAATGAGCTAAAT
CTGTTCTGCCAGACTCAGGGCAAGCAAGTATCCAGCGTTAAAGATCTGCCAGAGTGTCCT
GTAGACTTGGTCATTAACTGCTTGGATTGCAATGAAAACGCCTTCTTGTGTGACCAACCC
TGGTACCGAGCAGCTGTAGACTGGGCCAACCACAACCGGGCTCCTGTGCTAAATATTGAC
CCTCCTGTGGGTGACCATGTGCAGGGCATTCATGCCAAGTGGTCCCTGGAGCTGGGCTTG
CCACTTGCTTTAGGGGAACAGGCCGGGCGCATCTACTTGTGTGACATTGGCATCCCACAG
AAGGTTTTTAGGGAGGTGGGCATTAGCTACCACTCCCCCTTTGGCTGCAAGTTTGTGATC
CCGTTACATTCCATGTAG

Protein sequence predicted based on nucleotide sequence

MAADWLGSIVSINCGNTLGVYQGCVSAVDRINQTISLSQPFHNGVKCLVPEVTFRAGDIS
ELKILEIPSESLKYTSDQLNDHSTGFGYLPTRQQNGTGKQKQTVAHNSAQNIPGQEEGKA
SEPSSTSPQPCSKSFVDRHNEAVNQPKNFRRRHNSLGSSSSRYPNQVTPKKSGTKNGQLK
ARDDECFGDELEEIPDTDFDFEGNLALFDKAAVFEEIDTHERRGGGGTRSRGTPNERPPT
YRHDENILESEPIVYRKIVVPQSGGQEYCTDSGLVVPSISYVLHKKLLCVAEKHGLSVER
RLEMSGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANYDVD
IILFLPNFVKMLEPVTNELNLFCQTQGKQVSSVKDLPECPVDLVINCLDCNENAFLCDQP
WYRAAVDWANHNRAPVLNIDPPVGDHVQGIHAKWSLELGLPLALGEQAGRIYLCDIGIPQ
KVFREVGISYHSPFGCKFVIPLHSM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable