ggcx (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACAATTCTATGTGCTCTGGTTCGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACCAATCTTGTGGTTGTG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709297
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1506nts
Open reading frame: 1 to 1506

ATGACCAATCTTGTGGTTGTGCACTGGGCAGGCCTTATATTGGACTTGACCGCTGGCTAC
TTGCTGTTCTTTGATGTGACTCGTCCTGTGGCTTTGTTTTTTGTCAGTTATTTTCACTGC
ATGAATTCCCAGCTCTTCAGCATAGGCATGTTCTCATACACCATGCTGGCAACCAGCCCT
CTTTTCTGCTACCCTGATTGGCCCAGAAGGCTGATTGCTAAACTTCCCCGCTGCCTGCAG
TATGTCCTGCCACAGACACAGCCCCCGCAGGAGAGTATGGAATGTGTGTACACTGGACGG
AAGGGAAAGACCGCTTCCCAGCCTGGATTGTGGCACAAGCTTGGAGCAGCCTTTACTGTG
CTTTACATTATAGAGCAGCTCTTCTTGCCTTACTCCCATTTTATCACTCAGGGCTACAAT
AATTGGACAAATGGACTATACGGATATTCGTGGGATATGATGGTTCACTCACGGTCACAC
CAGCATGTTAAAATCACCTACAGAGATGGAATCACTGGGGAAATTGGATACCTTAACCCT
GGGGTATTCACACAGACGAGGCGCTGGAAAGATCATGCGGACATGCTGAAGCAGTATGCG
GTCTGCCTGCAAGGTCTGCTGGGCGCATACAACATCTCACAACCAGAGATCTACTTCGAT
GTGTGGGTGTCTATCAACGACCGCTTCCAGCAGAGGCTGTTTGATCCTACTGTGGACATT
GTAAAAGCTGAGTGGTCTCCATTCCATAAGACTCCCTGGCTGAAGCCGCTGCTGGTTGAT
CTTTCACCTTGGAGGGCAAAGTTTCAGGAGGTGGAGGATTCTCTGGATAATCAGACGGAT
GTAGTGTTCATTGCAGATTTCCCAGGCTTATTTTTGGAGAACTTCGTAAGTGAGGATCTG
GGTAATACAAGCCTACATATCCTTCAGGGTGAGGTTAATGTGGAAATAGTAAAGGAGGAG
AGGAACGTCACTTTAAGAGAGGGAGACCACATGCAGTTGCCAGCTGGTGAATATCATAAA
GTATTCACAGTGTCTCCAGGCCCTTCCTGTTACATGTACATCTATGTAAATACTACGGCT
GTGACCCTGGAGAAAAATTTTACACGGCTACTAGAACTGAAGGAGAGGGCTGAAAATGGA
AGTGAAACTTTCCAGCTTCCTCCTGAACTCCAGCCCATTATCTCGGGTACAGTGGAGGAC
GTCCATAACACAGACCCGCTTGTTCTTGCCTACCTGCGCAGGCAGAAGAGGCTGGAGCTG
TCAGAAAGGAGACGAAACGCAACTTTATTGCAGAGGGTTGATCGTTTCTGCGGTAAAAAA
TACTACTTGCTACGTCGAAGCTTCCTAATGACAGCAATTGCAATGAGGAACTTGGCATTA
GGTCGTCCATCACTGGAACAGCTGGCAGAGGAAGTCTCATTTGCAAATGTAAAGTATGAA
GACCCTTTCATGAAAAGTGAAAAGCAGCAGGACAGCCTACATCACGAACCAGAGCACATA
GAATTGTGA

Protein sequence predicted based on nucleotide sequence

MTNLVVVHWAGLILDLTAGYLLFFDVTRPVALFFVSYFHCMNSQLFSIGMFSYTMLATSP
LFCYPDWPRRLIAKLPRCLQYVLPQTQPPQESMECVYTGRKGKTASQPGLWHKLGAAFTV
LYIIEQLFLPYSHFITQGYNNWTNGLYGYSWDMMVHSRSHQHVKITYRDGITGEIGYLNP
GVFTQTRRWKDHADMLKQYAVCLQGLLGAYNISQPEIYFDVWVSINDRFQQRLFDPTVDI
VKAEWSPFHKTPWLKPLLVDLSPWRAKFQEVEDSLDNQTDVVFIADFPGLFLENFVSEDL
GNTSLHILQGEVNVEIVKEERNVTLREGDHMQLPAGEYHKVFTVSPGPSCYMYIYVNTTA
VTLEKNFTRLLELKERAENGSETFQLPPELQPIISGTVEDVHNTDPLVLAYLRRQKRLEL
SERRRNATLLQRVDRFCGKKYYLLRRSFLMTAIAMRNLALGRPSLEQLAEEVSFANVKYE
DPFMKSEKQQDSLHHEPEHIEL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable