mios (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAGGCTGAACATTCTCTGCTGG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGTGGCTCTAAGCCTG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709421
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2640nts
Open reading frame: 1 to 2640

ATGAGTGGCTCTAAGCCTGACATCCTCTGGGCACCTCACCATGTGGACAGATTTGTAGTA
TGTGATTCTGAGCTCAGCCTTTACCACATAGAATCTGCAGCAAGCCCCAGCTCAGAATTA
AAAGCAGGATCCTTGCGGCTGTCTGAAGAGACCACTGCTACTTTACTGGCTATAAACTCT
GATACTCCTTACATGAAATGCGTAGCTTGGTATCCCAAGTATGATCCAGAATGTCTTTTG
GCTGTTGGTCAGGCCAATGGAAGAGTTGTGCTAACAAGTCTGGGCCAGGACCACAACTCT
AAATGTAAAGACTTGATAGGAAAGGAGTTTGTCCCCAAGCATGCACGTCAGTGTAACACG
CTGGCATGGAACCCCCAGGACAGTAATTGGCTTGCGGCTGGGTTAGATAAACACCGGGCT
GACTTTTCAGTATTAATCTGGGACATCAGCAGCAAGTACACTCCAGAGGTAGCAGTTCCG
GCAGAGAAAGTTCGTCTTGCGCCCGGTGATAGTGAAACGTGTTCAGTGGTTACCAAACCA
CTTTATGAACTAGGACAGAACGATGCTTGCCTCTCTCTTTGCTGGCTTCCCAGAGACCAG
AAACTGCTTTTGGCTGGAATGCATCGTAACTTGGCAATATTTGACTTGAGGAACACAAAT
CAGAAAATGTTTGTAAATACTAAAGCTGTCCAAGGAGTGACCGTTGACCCACATTTCCAT
GACCGAGTAGCTTCATTTTTTGAAGGACAAGTTGCCATTTGGGATCTTCGAAAATTTGAA
AAACCAGTCTTAACTCTAACAGAGCAACCAAAACCCCTAACTAAAGTAGCCTGGTGCCCA
ACTAGAACTGGACTTCTGGCTACTCTAACTAGAGACAGTAATATAATCAGACTGTACGAC
ATGCAGCACACTCCAACACCCATTGGGGATGAAACAGAGCCTACTATCATTGAAAGGAGT
GTCCAGCCTTGTGATAACTACATTGCATCTTTTGCTTGGCACCCTACAAGCCAGAACAGA
ATGGTCGTTGTTACTCCTAATAGGTCAATGTCAGATTTTACTGTATTTGAGAGGATCTCT
TTGGCTTGGAGCCCCACAACTTCACTGATGTGGGCTTGCAGCAGGCAGTTGTATGAGTGC
ACAGAGGAAGGAAAAGGCAGCTCTTCATTAGATCGAGATATAGCCACTAAAATGAGACTC
CGAGCTTTATCGCGATATGGGCTCGACACGGAGCAGGTTTGGAGAAACCATCTGTTGGCA
GGAAATGATGATCCTCAGTTGAAATCCTTGTGGTACACTTTACACTTTATGAAGCAGTAT
GCAGAAGATATTGACCAAAAATCTTCAGGCAACAAAGGGTTATTGGTGTATGCTGGAATC
AAAGCAATCACAAAGTCATCTATGGGGACAACTGAAAGTTTCAGGCACAGCTGGACCGGC
TCTGATCGACAAGCAGATATTGTGCATTATTCCAGTGAGGAAAGATGCCTCGCCTTACAG
CTCTGTGGCTGGAGAACAAAGGGTGCTGATTCTGACATGGGGCCTTTTCTGAACTCCTTA
GAGCAGGAAGGAGAATGGGAGAGAGCAGCTGCTATAGCACTGTTCAATCGAGATATTCGC
CGTGCCATACAGATCCTAAATAAGGGAGCATCCACTGGAAAAGGTAATCTTAACCTAAAC
GTCGTAGCAATGGCGCTGTCAGGTTATACTGATGAGAAGAAATCCTTGTGGAGAGAAATG
TGCAGCACTTTAAGGCTACAGCTTAACAACCCATATCTTTGTGTTATGTTTGCTTTTCTA
ACAAGTGAGCCTGGGGTATATGATGGTGTTCTGTATGAGAACAGCATGGCAGTGAGGGAT
CGTGTGGCATTTTCATGTTTGTTCCTTAATGATGCTCAGTTAAGCAGATACTTTGACAAG
CTGACTAACGAAATGAAGGAAGCTGGAAACCTGGAAGGAATTTTACTAACGGGACTGACA
AAGGATGGAGTTGACTTAATAGAAAGTTACGTTGATCGAACGGGAGATGTTCAGACAGCA
AGCTACTGTATGTTGCAGGGATCTCCATCTGATGTCCTCAAAGATGAAAGAGTTCAGTAT
TGGATAGAGAGCTATAGGAATCTGCTGGATGCATGGAGATTTTGGCACAAGCGAGCGGAG
TTTGATATTCATCGAAGTAAGCTTGACCCAAGCTCCAAGCCATTAGCCCAGGTGTTTGTG
AGCTGCAATTTCTGTGGTAAGTCCATCTCCTACAGTTGTTCGTCTGTACCTCACCAAGGC
CGTGGATTCAGTCAGTATGGCGTCAGTGGGTCACCAACTAAGTCTAAATTCACCAGCTGT
CCTGGATGCCGCAAGCCTCTTCCCCGCTGTGCACTTTGTCTCATAAATATGGGGAATCCT
GTCTCCAGTAGCCCAGGAGCATCAAAGTCGGATGAGAAGGTGGACCTGAGCAAGGAAAAG
AAAGTGGCAAAATTTAACAACTGGTTTACATGGTGTCACAACTGCCGACATGGTGGCCAT
GCGGGACATATGCTAAGCTGGTTCAAAGATCACAGCGAGTGCCCGGTCTCTGCGTGTTCA
TGTAAGTGTATGCAGTTGGACACAACTGGAAATCTTGTCCCAGCAGAGAATGTTCAGCCT
TAA

Protein sequence predicted based on nucleotide sequence

MSGSKPDILWAPHHVDRFVVCDSELSLYHIESAASPSSELKAGSLRLSEETTATLLAINS
DTPYMKCVAWYPKYDPECLLAVGQANGRVVLTSLGQDHNSKCKDLIGKEFVPKHARQCNT
LAWNPQDSNWLAAGLDKHRADFSVLIWDISSKYTPEVAVPAEKVRLAPGDSETCSVVTKP
LYELGQNDACLSLCWLPRDQKLLLAGMHRNLAIFDLRNTNQKMFVNTKAVQGVTVDPHFH
DRVASFFEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYD
MQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMVVVTPNRSMSDFTVFERIS
LAWSPTTSLMWACSRQLYECTEEGKGSSSLDRDIATKMRLRALSRYGLDTEQVWRNHLLA
GNDDPQLKSLWYTLHFMKQYAEDIDQKSSGNKGLLVYAGIKAITKSSMGTTESFRHSWTG
SDRQADIVHYSSEERCLALQLCGWRTKGADSDMGPFLNSLEQEGEWERAAAIALFNRDIR
RAIQILNKGASTGKGNLNLNVVAMALSGYTDEKKSLWREMCSTLRLQLNNPYLCVMFAFL
TSEPGVYDGVLYENSMAVRDRVAFSCLFLNDAQLSRYFDKLTNEMKEAGNLEGILLTGLT
KDGVDLIESYVDRTGDVQTASYCMLQGSPSDVLKDERVQYWIESYRNLLDAWRFWHKRAE
FDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSSVPHQGRGFSQYGVSGSPTKSKFTSC
PGCRKPLPRCALCLINMGNPVSSSPGASKSDEKVDLSKEKKVAKFNNWFTWCHNCRHGGH
AGHMLSWFKDHSECPVSACSCKCMQLDTTGNLVPAENVQP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable