sox17a-b (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  sox17a-b
Gene Name:  SRY (sex determining region Y)-box 17 alpha
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGCACTGGGGTAGTTGCAGTA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGCAGCCCTGATGGT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709544
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1131nts
Open reading frame: 1 to 1131

ATGAGCAGCCCTGATGGTGGCTACGGCAGCGACGATCAGAATCAGGGAAAGTGCTCAGTA
CCAATAATGATGAGTGGACTCGGGCAGTGCCAGTGGGCCGAACCCATGACTTCTCTCGGG
GAGGGGAAACTGAAAAGCGATGCCAATTACAGAAGCAAAGCCGAAACTCGGATCCGCCGA
CCTATGAATGCCTTTATGGTGTGGGCAAAGGACGAGCGCAAGAGGCTGGCGCAACAGAAC
CCCGACCTGCACAACGCCGAGCTCAGCAAGATGCTTGGCAAGTCGTGGAAGGCTCTGAGC
CTGGCAGAGAAGCGGCCCTTTGTGGAGGAGGCAGAGAGGCTGAGGGTGCAGCACATGCAG
GACCACCCCAACTACAAGTACAGACCCCGCAGGAGGAAACAGGTGAAGAGGATGAAGAGG
GCAGAGAACGGCTTCATGCACATGACAGAGGCGCAGGAGAGCGCAGTTATGGGCGCTGAT
GGAAGGATGTGCCTGGAGAACTTCAACTTGGGTTTCCATGAGCAGACTTACCCCCAGCTT
CCCCAGGCCAGCCACTACAGGGAACCCCAAGCCATGGCTCCACATTATGATGGGTACAGT
TTACCCACTCCTGAGAGCTCCCCTCTGGATTTGGCTGAGGCAGATCCTGTGTTCTTCACA
TCTCCAGCCCAGGACGAGTGCCAGATGATGCCCTACAGCTACAACTCCAGCTACACTCAC
CAGCACAACTCTGGCGCCTCTATGTTGGTAAGGCAAATGCCACAAACTGAGCAAATAGGT
CAAGGCAGTCCAGTGGAGGGTATGATGGCATGCCAGAGCTCCCCACACATGTACTATGGA
CAGATGTACCTGCCAGGCTCTACCAGGCATCACCAGCACCCCCAGGCTGGGCAGCCTTCC
CCTCCCCCCGAGGCTCAGCAGTTGGGCAGAGCAGATCAAACCCAACAGGCTGACATGATG
GCAGAGGACAGGACTGAATTCGAGCAGTATCTCAGCTATGTGTCTAAGTCAGACCTGGGC
ATGCATTACCACGGGCAGGAATCAGTGGTGCCAACTGCAGACAATGGGCCCATCTCTTCT
GTCCTTTCCGATGCCACTACTGCTGTATATTACTGCAACTACCCCAGTGCCTGA

Protein sequence predicted based on nucleotide sequence

MSSPDGGYGSDDQNQGKCSVPIMMSGLGQCQWAEPMTSLGEGKLKSDANYRSKAETRIRR
PMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGKSWKALSLAEKRPFVEEAERLRVQHMQ
DHPNYKYRPRRRKQVKRMKRAENGFMHMTEAQESAVMGADGRMCLENFNLGFHEQTYPQL
PQASHYREPQAMAPHYDGYSLPTPESSPLDLAEADPVFFTSPAQDECQMMPYSYNSSYTH
QHNSGASMLVRQMPQTEQIGQGSPVEGMMACQSSPHMYYGQMYLPGSTRHHQHPQAGQPS
PPPEAQQLGRADQTQQADMMAEDRTEFEQYLSYVSKSDLGMHYHGQESVVPTADNGPISS
VLSDATTAVYYCNYPSA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable