sox17a-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGCACTGGGGTAGTTGCAGTA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGCAGCCCTGATGGT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709654
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1140nts
Open reading frame: 1 to 1140

ATGAGCAGCCCTGATGGTGGTTACGCCAGCGACGATCAGAATCAGGGAAAGTGCTCAGTG
CCAATAATGATGACTGGACTCGGGCAGTGCCAGTGGGCCGAACCCATGACTTCTCTCGGG
GAGGGGAAACTGAAGAGCGATGCGGGATCTGCCAATTCTAGGAGCAAAGCCGAGGCTCGG
ATCCGCCGGCCTATGAATGCCTTCATGGTGTGGGCAAAGGACGAGCGCAAGAGACTGGCA
CAGCAGAACCCCGACCTGCACAACGCCGAGCTCAGCAAGATGCTTGGCAAGTCGTGGAAG
TCTCTGACTCTGGCAGAGAAGCGCCCCTTTGTGGAGGAAGCAGAGAGGCTGAGGGTGCAG
CACATGCAGGACCACCCCAACTACAAGTACAGACCCCGCAGGAGGAAACAGGTGAAGAGG
ATGAAGAGGGCAGAAAACGGCTTCATGCACATGGCAGAGGCGCAGGAGAGCGCAGTGCTG
GGCGCAGATGGAAGGATGTGTCTAGAGAACTTCAGCCTGGGTTATCATGAGCAGACTTAC
CCCCACGGCCAGGTTCCTCAGAGCAGCCACTACAGGGAACCCCAAGCAGTGGCTCCCCAT
TATGACGGTTACAGTTTGCCCACTCCTGAGAGCTCTCCTCTGGATTTGGCAGAAGCAGAT
CCCGTGTTCTTCACATCTCCAGCCCAGGACGAGTGCCAGATGATGCCCTACAGCTACAAT
GGCAGCTACCCTCACCAGCAGAACTCCATGTTGGTAAGACAGATGCCACAAACTGAGCAA
ATGGGTCAAGTCAGTCCAGTTCAGGGTATGATGGCATGTCAGAGCTCCCCACACATGTAC
TATGGACAGATGTACTTGCCAGGCTCTGCCAGGCATCACCAGCTCCACCAAGCTGGGCAG
CCCTCCCCACCCCCTGAGGCTCAGCAGATGGGCAGAGCAGATCACATCCAACCGGCTGAC
ATGTTGGCAGAGGTGGACAGGACTGAATTCGAGCAGTATCTGAGCTATGTGGCCAAGTCA
GACCTGGGTATGCATTATCATGCGCAGGAATCGGTGGTGCCTACTGCAGACAATGGGCCC
ATTTCTTCTGTCCTTTCCGATGCCAGTACTGCTGTATATTACTGCAACTACCCCAGTGCC
TGA

Protein sequence predicted based on nucleotide sequence

MSSPDGGYASDDQNQGKCSVPIMMTGLGQCQWAEPMTSLGEGKLKSDAGSANSRSKAEAR
IRRPMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGKSWKSLTLAEKRPFVEEAERLRVQ
HMQDHPNYKYRPRRRKQVKRMKRAENGFMHMAEAQESAVLGADGRMCLENFSLGYHEQTY
PHGQVPQSSHYREPQAVAPHYDGYSLPTPESSPLDLAEADPVFFTSPAQDECQMMPYSYN
GSYPHQQNSMLVRQMPQTEQMGQVSPVQGMMACQSSPHMYYGQMYLPGSARHHQLHQAGQ
PSPPPEAQQMGRADHIQPADMLAEVDRTEFEQYLSYVAKSDLGMHYHAQESVVPTADNGP
ISSVLSDASTAVYYCNYPSA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable