pklr (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACGGCACCCTCACCACCT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCGCAGCTGATGCAGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709728
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1593nts
Open reading frame: 1 to 1593

ATGGCGCAGCTGATGCAGGACCTGGGACCGGCTTTCGTCCAACGGCAGCAGCTGAATGCC
TCAATGGCCGACACCTTCCTGGATCACATGTGTCTGTTGGACATTGACTCGGAGCCCATA
ACGGCCAGAAACACCAGCATTGTCTGTACCATCGGTCCAGCCTCGCGCTCTGTGGAAATG
CTGAAGGAGATGATAAAGGCTGGGATGAACATCGCTCGTCTGAACTTCTCTCACGGGAGC
CATGAGTACCACGCTGGGTCTATTCACAATATTCGGGAAGCCACCGAGAGCTTTGCCTCA
AATCCCATTCACTACCGGCCTGTGGCTATCGCTCTGGACACCAAGGGGCCAGAAATCCGA
ACTGGGGTCATCAAGACGGGGGAGAACACAGAGGTGGAGCTGATGAAAGGCTCAATTATG
AAAGTGACTAATGACGAGTCCTTCAAGGATAAGTGTGACGAGAAGATCCTGTGGGTGGAT
TATAAGAACATCTGTAAAGTTGTGAAGGTCGGAGGCAGGATTTTTGTGGACGATGGACTG
ATTTCTCTGTTGGTTAGAGAGATTGGCCCTGATTACTGCATGGCTGAAGTGGAGAATGGG
GGTAACCTGTGCAGTAAGAAAGGGGTGAATTTGCCTGGGGCTCCGGTGGACCTTCCTGCA
TTATCGGAGAGAGATTGCCTGGACTTGAAGTTTGGGATTGAGCAGGGAGTAGACATGGTA
TTTGCTTCCTTCATTCGCAAGGCGCAAGATGTACACACCATTCGCAAGGAACTGGGAGAA
AAAGGTCGGAATATTCGCATCATCAGCAAAATAGAAAATCATGAGGGAGTCAAGAGGTTC
GATGAGATTCTCGAGGCCAGTGATGGGATAATGGTGGCCCGGGGTGATCTCGGCATTGAA
ATTCCAGCAGAGAAGGTTTTCTTGGCACAGAAAATGATGATTGGACGTTGCAACAGAGCC
GGGAAGCCAGTGATCTGTGCCACGCAGATGTTGGAGAGTATGATCAAGAAGCCTCGTCCG
ACCCGTGCGGAGAGCAGTGATGTGGCCAATGCTGTGCTGGATGGAGCTGACTGTATCATG
TTGTCTGGAGAGACTGCCAAGGGCCTGTACCCAGTGGAGTCTGTACACATGCAGCATGCG
ATTGCCCGGGAGGCTGAGGCAGCGATCTACAATCGCCAGCTGTTTGAGGAACTCAGACGA
GTGACCCCACTGACCCAGGATCCCACAGAGGTGACTGCCATAGGGGCTGTAGAGGCCTCG
TTTAAATGCTGTGCTGGAGCCATCATTGTTCTGACCACGTCTGGCCGGTCTGCGCAACTC
CTTTCTCGTTACCGCCCTCGTGCTCCCATCATTGCAGTGACACGTAGTGCCCAGGTGGGG
CGCCAGGCTCATCTCAACCGCGGGGTCTTCCCTGTCCTGTACCAGGAGGCGCAGCTGGAA
GTTTGGGCTGATGATGTGGATCGTAGGGTGCAGTTTGCCATTTCCATTGGAAAAGTCCGT
GGGTTCCTGAGCAAAGACGACATTGTGATTATTGTGACTGGCTGGAGGCCTGGGTCTGGC
TACACCAATATCATGCAGGTGGTGAGGGTGCCGTAA

Protein sequence predicted based on nucleotide sequence

MAQLMQDLGPAFVQRQQLNASMADTFLDHMCLLDIDSEPITARNTSIVCTIGPASRSVEM
LKEMIKAGMNIARLNFSHGSHEYHAGSIHNIREATESFASNPIHYRPVAIALDTKGPEIR
TGVIKTGENTEVELMKGSIMKVTNDESFKDKCDEKILWVDYKNICKVVKVGGRIFVDDGL
ISLLVREIGPDYCMAEVENGGNLCSKKGVNLPGAPVDLPALSERDCLDLKFGIEQGVDMV
FASFIRKAQDVHTIRKELGEKGRNIRIISKIENHEGVKRFDEILEASDGIMVARGDLGIE
IPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAESSDVANAVLDGADCIM
LSGETAKGLYPVESVHMQHAIAREAEAAIYNRQLFEELRRVTPLTQDPTEVTAIGAVEAS
FKCCAGAIIVLTTSGRSAQLLSRYRPRAPIIAVTRSAQVGRQAHLNRGVFPVLYQEAQLE
VWADDVDRRVQFAISIGKVRGFLSKDDIVIIVTGWRPGSGYTNIMQVVRVP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable