ube3d (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACATCTTCAAATAAGAAACCTGCC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGAGCTGCAGACAGAT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709943
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1233nts
Open reading frame: 1 to 1233

ATGGAGCTGCAGACAGATGCTGCTGTGTGTCCGGCCTCTGGGGCCACTTCCCATGTGAGG
GACACAGGGACCAACTCGCAGGGCGACTGTGACATTGTTGTTCGTCTGGAAATTCGGAAA
AACATGCAGACTGCTAGTCTCATTGTGGGTGGCTTAGGAAGCTTAATAGTGGATGTGTCT
CTGAGCCCGTCATTTTTACAGATACAAACTTCTGAGCAGAGCGAGAGGTTCTGTATCCCA
GCAGATGTGAAGTTGCTTCCTTCATCTTGCCGTATAATGCATCAGATTGCTGGAGAAGGA
TTATACATGCAGCTGAAGGTGGAAACTGCCAACAATACAGACATGCCTCTATTGCTGAAG
GAAATCCTGAAGATTCAGAATAACTACACTTTTTACTGCCAGTCATGCGGAGAGGTCATA
ATCAAAGAACAACAATTCTTTAGAGTTCTTCCACTACCTGGTGAAAACTGGAGCTCCCTG
ATTGAAGAATGGTGCTGCCATCCTGATCCCTTTGCCAATCGCAGTTGCTTGCCCAAGGTG
AACGACTGTTTTCTCGGAGACACTTTCCTTTTGTTGAATACAGCAAATATTTCAGACCCC
ATTGTAGAGAATAATGAGAAGTCATCTGTTCCTCGTTTGGCTGATGCTAATGCCTTGAAA
TCAAAAGAAAATACTAAAGTTATTTGCAAACGATGCAAGGCAATGCTGGGAGAGAAAGTG
TCATCAGATGCTATCAAGTACTATATCACAGAAATAATGATTCACCCATCAAGCCAAGGT
TATTGCATGATATCAAGAATGCAGTTTTTGGAAAGTATTTTAGTGCGGCTTCTCATGGAA
CACTCTAACTGTAGGAGTACTTTTAGGTTCTGCATTCAAGAGTCAGATGGCAAGCACTTT
CTTTTGCTTTGGCTTTTGAATACGGATACATTATTGGTGGAATCACCAAGAAAGCCAGGG
ACTCAGAATAATTGTTTCTTTTCTGACAATAATGTCATGGCTTCTTCAAGAGCCCTAGAG
GCAAGAAATGCTATGCGGGTACTCTTCCTACCTTGCTCGGAAAACAGGAACAAACAGCTT
GCTGATAAGTGGATGAATGATACTGGTGCTCATCCATTAACATTCCCTCTAAACACATGC
TTGGAACTTATCCTGCTGCTGTCACTCAACAATACATCCTTGCCTAACTCCCTGCGCTTC
ATGAATAACTGGCAGGTTTCTTATTTGAAGATGTGA

Protein sequence predicted based on nucleotide sequence

MELQTDAAVCPASGATSHVRDTGTNSQGDCDIVVRLEIRKNMQTASLIVGGLGSLIVDVS
LSPSFLQIQTSEQSERFCIPADVKLLPSSCRIMHQIAGEGLYMQLKVETANNTDMPLLLK
EILKIQNNYTFYCQSCGEVIIKEQQFFRVLPLPGENWSSLIEEWCCHPDPFANRSCLPKV
NDCFLGDTFLLLNTANISDPIVENNEKSSVPRLADANALKSKENTKVICKRCKAMLGEKV
SSDAIKYYITEIMIHPSSQGYCMISRMQFLESILVRLLMEHSNCRSTFRFCIQESDGKHF
LLLWLLNTDTLLVESPRKPGTQNNCFFSDNNVMASSRALEARNAMRVLFLPCSENRNKQL
ADKWMNDTGAHPLTFPLNTCLELILLLSLNNTSLPNSLRFMNNWQVSYLKM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable