lmnb2-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  lmnb2-a
Gene Name:  lamin B2
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACATGACAGAGCAGCCTCTGGAT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTACTGCTACACCAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710229
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1872nts
Open reading frame: 1 to 1872

ATGGCTACTGCTACACCAAGTCGGTCGGCGGCCTCATCCATGCAATCTCCTGCCCGGGGA
ATATCATCTACCCCTCTTTCACCAACTCGCATATCTAGGCTTCAGGAGAAGGAGGAGTTG
AGACATCTCAATGACCGACTAGCCGTCTATATAGACCGCGTGCGTGCACTGGAGCTGGAA
AATGACCGGCTGATGGTTAAGATTTCTGAAAAAGAGGAGGTTACCACTCGGGAGGTTAGT
GGCATCAAGAACCTGTATGAATCAGAGCTTGCTGATGCGCGGAAGGTTTTGGATGAGACA
GCCAGAGAGAGAGCACGGTTGCAAATAGAACTGGGAAAATTTCGTTCAGATCTTGACGAG
CTAACAAAAAACTACAAAAAAAAAGATGCTGACCTGTCCACGGCACAGGGGCGTATTAAA
GACTTGGAAGCTCTCTTTCACCGTAGTGAAGCAGAACTTGGAACTGCACTGGATGAGAAG
CGTAGTTTGGAGGCAGAGGTAGCAGATCTTCGTGCCCAGCTCTCCAAGGCTGAAGATGCC
CATGCTGTTGCTAAAAAACAGCTGGAAAAGGAGACCCTGATGAGAATTGACTTTGAAAAT
CGCATCCAGAGTTTGCAGGAGGAGATGGACTTCAGAAAGAATATTTATGAGGAGGAGTCC
CGTGAAACAAGGAATCGTCATGAACGGCGTATAGTAGAAGTGGACAGGGGTCATCACTAT
GATTATGAATCCAAATTGGCACAAGCATTAGATGAACTTCGAAAACAACATGATGAGCAA
GTTAAGATGTACAAGGAAGAACTAGAACAAACCTATCAGGCTAAGCTTGACAACATCAAA
CGTTCTTCTGACCACAATGATAAAGCTGCCAACACTGCCCGAGAAGAGCTGACTGAGGCA
CGCATGAGAATTGAAACGCTTAGTTACCAGCTGTCAGGCCTGCAGAAACAGGCAAATGCT
GCAGAGGAACGTATACGTGCGTTAGAAGAGTTGCTAAGCAGTGATCGTGACAAGTATCGT
AAGCTTTTGGACTCCAAAGAAAGAGAGATGGCTGAAATGAGGGACCAGATGCAGCAGCAA
CTTACTGAGTATCAAGAGTTGCTTGATGTCAAACTTGCACTTGATATGGAGATAAATGCT
TACCGCAAGCTGCTTGAAGGAGAAGAGGAAAGGTTAAAGCTTTCCCCAAGCCCGCAATCA
AGAGTCATTGTCTCCAGGGCTACTTCCAGTAGTGCTTCTGCCACCCGTACCTCTAGAAGC
AAAAGAAAGCGCATTGAAGAGGAATATGAGGAAGGTGGGGCAAGTACAGGCTTGCGCACT
GGCACTTCCCTCGGTTCAAGTCACATCACTGCTAGTGAAGGCTCTAGCCGCACTGTTACT
AGTGGGCAGATTAGTGGTACTCAGTTTCACTTGTCCCAGCAGGCTTCGGCCACAGGAAGC
ATCAGTATTGAGGAGATCGATTTGGAAGGAAAATATGTCCACCTTAAAAATAACTCAGAT
AAGGACCAGTCCCTTGGTAACTGGAGGCTGAAAAGAAAAATTGGAGAGGAAGAGGAAATT
GTTTACAAATTCACCCCTAAATATGTACTAAAAGCAGGCCAGTCTGTAAAAATCTACAGT
GCTGATGCTGGTGTGGCTCACAGCCCCCCTTCCATTTTAGTGTGGAAGAACCAGAGTTCC
TGGGGCACAGGCTGCAACATTAGAACATACCTGGTTAACACCGAAGAGGAGGAAGTAGCT
GTGAGAACAGTTACAAAGTCTGTCTTAAGAAATGTAGAGGAGGAAAAGGATGAAGAAGCA
GACTTTGGTGAGGAAGATCTCTTTCACCAGCAGGGTGATCCAAGAACAACATCCAGAGGC
TGCTCTGTCATGTAA

Protein sequence predicted based on nucleotide sequence

MATATPSRSAASSMQSPARGISSTPLSPTRISRLQEKEELRHLNDRLAVYIDRVRALELE
NDRLMVKISEKEEVTTREVSGIKNLYESELADARKVLDETARERARLQIELGKFRSDLDE
LTKNYKKKDADLSTAQGRIKDLEALFHRSEAELGTALDEKRSLEAEVADLRAQLSKAEDA
HAVAKKQLEKETLMRIDFENRIQSLQEEMDFRKNIYEEESRETRNRHERRIVEVDRGHHY
DYESKLAQALDELRKQHDEQVKMYKEELEQTYQAKLDNIKRSSDHNDKAANTAREELTEA
RMRIETLSYQLSGLQKQANAAEERIRALEELLSSDRDKYRKLLDSKEREMAEMRDQMQQQ
LTEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPQSRVIVSRATSSSASATRTSRS
KRKRIEEEYEEGGASTGLRTGTSLGSSHITASEGSSRTVTSGQISGTQFHLSQQASATGS
ISIEEIDLEGKYVHLKNNSDKDQSLGNWRLKRKIGEEEEIVYKFTPKYVLKAGQSVKIYS
ADAGVAHSPPSILVWKNQSSWGTGCNIRTYLVNTEEEEVAVRTVTKSVLRNVEEEKDEEA
DFGEEDLFHQQGDPRTTSRGCSVM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable