Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  None
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATACCTTCCCTGGTACAAAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGATTTTATTGTGACAACTTC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710292
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1011nts
Open reading frame: 1 to 1011

ATGGGATTTTATTGTGACAACTTCATGTTCTCTCAGCAGAACCTGCACCACAGCCAGAGG
GCCCCCAACTTCAGCATCGGGGGGGAATTCACCCCTCCAGCTAATCCTTACCTGTGGCTG
GGGGGTCCTGGGATGAATAACGCCCCCAATTACAGCCCTGCCCCTGCTCCTTACATTCCC
TCTGCTTTCAGCGCCCCCCAGCGGCAGTTCATGGCCAACTCTGCAGCCTTTGGTGGAGCC
GATTTGGGTTGGATGTCGGCGGCTTCCCAGGAGGAGCTTCTCAAGATGGTGAGACCCCCT
TACTCGTACTCTGCCCTCATCGCCATGGCCATCCAGAATGCCTCAGACAAGAGGCTCACC
CTGAGTCAGATTTACCAATATGTGGCAGAAAACTTCCCTTTCTACAAGAAGAGCAAAGCA
GGCTGGCAGAACTCCATCAGGCACAATCTGTCCCTCAATGACTGCTTCAAGAAGATGCCA
AGGGATGAGAATGATCCAGGAAAAGGAAACTACTGGACTCTGGACTCCAACTGCGAGAAG
ATGTTTGACAATGGCAATTTCCGCCGCAAGAGGAAGCCCAAATCCGAGTCCAACAACGCT
AAAATCGCCAAGAGGGATGAGGATCACTTGAACCCAAAAGGCAAAGAAAGTCCACCCATG
ATTACTCCCTCCTCCTCCCCCGAGGTTCTGTCTCCCACAGGCCATAGCAAGAGCCCCTCT
CCCCCAACTGTCACCTACACCCCTTGTCTAACCAACTTCATTGGCAGCATGACTGCCGTG
GACTCAGCTACAATGAACAGACAGAGTCCCCTGGGGCTTCTCAATGAATTGTCTCAGAGG
AACATCACAGGCTTGAGCAGCTTCATTTCGGGCTCCGCAGTTGATCAGAGCTCAGAACAT
CAGGACAACAGTCTGTTCTACAACAGAAGCCCCTACTACACTAATCAGAAGCAGCCACAC
TTCCTTCAGCAGCTCCACCCACAGCAGCCCCCTTTGTACCAGGGAAGGTATTGA

Protein sequence predicted based on nucleotide sequence

MGFYCDNFMFSQQNLHHSQRAPNFSIGGEFTPPANPYLWLGGPGMNNAPNYSPAPAPYIP
SAFSAPQRQFMANSAAFGGADLGWMSAASQEELLKMVRPPYSYSALIAMAIQNASDKRLT
LSQIYQYVAENFPFYKKSKAGWQNSIRHNLSLNDCFKKMPRDENDPGKGNYWTLDSNCEK
MFDNGNFRRKRKPKSESNNAKIAKRDEDHLNPKGKESPPMITPSSSPEVLSPTGHSKSPS
PPTVTYTPCLTNFIGSMTAVDSATMNRQSPLGLLNELSQRNITGLSSFISGSAVDQSSEH
QDNSLFYNRSPYYTNQKQPHFLQQLHPQQPPLYQGRY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable