chrd (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  chrd
Gene Name:  chordin
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAAAACTCCATGGAGTCCTTG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCAGTGTCCCCCCATCCTACTT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710294
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2820nts
Open reading frame: 1 to 2820

ATGCAGTGTCCCCCCATCCTACTTGTGTGGACCCTGTGGATTATGGCTGTGGATTGCTCT
CGCCCAAAGGTTTTCTTGCCCATTCAGCCTGAACAGGAGCCCCTCCAATCCAAGACTCCA
GCAGGTTGTACATTTGGTGGGAAATTTTACTCCCTGGAGGATTCATGGCATCCAGATCTT
GGGGAACCATTCGGAGTTATGCACTGCGTACTATGCTATTGTGAGCCGCAGCGAAGTCGT
CGGGGAAAACCATCTGGTAAAGTCAGCTGCAAAAACATCAAACATGATTGCCCCTCACCC
TCCTGTGCTAACCCTATACTGCTCCCAGGACATTGCTGCAAGACTTGTCCCAAAGCTCCT
CCTCCTCCTATAAAGAAATCAGACTTTGTTTTTGACGGATTTGAGTATTTTCAAGAGAAG
GATGATGATCTTTATAATGATCGCTCTTATCTGAGTTCTGATGATGTCGCTGTGGAGGAG
AGTCGTTCAGAATATGTGGCACTACTAACAGCACCCAGCCATGTGTGGCCCCCAGTTACC
AGTGGAGTAGCCAAGGCTAGATTCAACCTGCAGCGCTCCAATTTGCTCTTCTCAATCACT
TATAAATGGATAGACAGACTTTCCAGAATCCGTTTCTCCGATCTTGACGGTTCTGTGCTG
TTTGAACACCCAGTGCACAGAATGGGATCCCCACGGGATGACACTATCTGTGGCATTTGG
AGGTCCCTTAATCGGTCCACTCTACGTCTTCTCCGAATGGGTCACATTCTTGTATCTCTG
GTGACCACAACACTTTCAGAGCCGGAAATATCAGGCAAGATTGTCAAACACAAAGCCCTA
TTTTCAGAGTCCTTCAGTGCACTTCTTACCCCAGAAGACTCTGATGAAACAGGAGGTGGA
GGACTCGCAATGCTAACACTGAGTGATGTGGATGACAATCTGCACTTTATACTTATGCTC
AGAGGTCTAAGTGGTGAAGAAGGAGATCAGATTCCAATACTTGTGCAAATCTCACATCAG
AACCATGTGATACGGGAGCTATATGCCAACATCTCTGCACAGGAACAAGACTTTGCAGAG
GTATTGCCAGACCTTTCCAGTCGAGAAATGCTGTGGCTGGCTCAAGGTCAGCTGGAGATT
TCAGTGCAGACAGAAGGGAGACGTCCGCAAAGCATGTCAGGCATAATCACAGTCAGAAAA
TCATGTGACACTTTGCAGAGTGTGTTATCGGGTGGTGACGCTTTAAATCCCACCAAAACT
GGAGCCGTGGGATCTGCAAGCATCACACTTCATGAAAATGGAACTCTGGAATATCAGATT
CAAATTGCTGGTACAATGAGTACTGTGACAGCTGTGACACTGGAGACAAAACCTCGCCGA
AAAACAAAGAGAAATATTCTGCACGATATGAGCAAGGACTACCATGATGGAAGGGTCTGG
GGATATTGGATAGATGCTAATGCCCGAGACCTACATATGCTGTTACAAAGTGAGCTCTTC
CTCAATGTAGCGACAAAGGACTTCCAGGAAGGGGAACTCAGGGGACAAATAACCCCTCTG
CTATACAGTGGCCTGTGGGCCAGATATGAGAAGCTCCCAGTTCCTCTAGCTGGTCAGTTT
GTGTCACCTCCCATCAGGACAGGTTCAGCAGGTCATGCATGGGTTTCACTGGATGAGCAC
TGCCACCTGCATTATCAGATTGTGGTGACTGGTCTGGGTAAGGCAGAAGATGCTGCACTG
AACGCACATCTACATGGTTTTGCTGAGCTTGGAGAGGTCGGTGAGAGCTCTCCTGGACAC
AAGAGGTTGTTAAAGGGCTTCTATGGGTCAGAGGCACAGGGTAGTGTAAAAGACCTTGAC
CTTGAACTATTGGGACATCTGAGCCGGGGCACAGCATTTATTCAAGTGAGCACCAAACTG
AATCCTCGTGGGGAAATTCGAGGACAGATACACATACCTAACAGCTGTGAATCTGGAGGA
GTTTCTCTAACCCCTGAAGAGCCTGAGTATGAATATGAAATATATGAGGAGGGAAGGCAG
CGCGACCCTGACGATCTTCGGAAAGACCCCAGAGCATGCTCTTTCGAAGGTCAACTAAGG
GCCCATGGTTCACGATGGGCTCCAGACTATGACAGGAAATGCTCTGTGTGCAGCTGTCAG
AAGCGTACCGTGATTTGTGATCCTATTGTGTGCCCACCTCTGAACTGCTCCCAGCCTGTC
CATTTGCCAGATCAGTGCTGTCCTGTGTGTGAAGAAAAAAAAGAAATGAGAGAGGTGAAA
AAACCAGAGAGGGCTCGCACAAGTGAAGGCTGCTTTTTTGATGGAGATCGCTCATGGAAG
GCAGCTGGTACACGTTGGCATCCTTTTGTTCCTCCATTTGGTCTAATTAAATGTGCCATT
TGCACCTGCAAGGGTTCCACTGGAGAAGTGCACTGTGAGAAGGTGACCTGTCCAAAACTT
TCCTGTACCAACCCAATCCGTGCCAATCCTTCTGATTGCTGCAAGCAGTGCCCAGAGGAG
CGGAGTCCTATGGAACTGGCAGACAGTATGCAGTCAGATGGAGCAGGATCATGCAGATTT
GGGCGTCACTGGTACCCAAATCATGAGCGTTGGCATCCAACTGTGCCACCCTTTGGAGAG
ATGAAATGTGTTACATGCACTTGTGCGGAGGGCATTACACAGTGTCGGAGACAGGAGTGT
ACAGGAACTACATGTGGTACTGGTTCAAAGCGGGACAGATGTTGCACCAAGTGCAAAGAT
GCCAATCAAGATGAAGATGAAAAAGTGAAATCAGACGAGACAAGGACTCCATGGAGTTTT
TAG

Protein sequence predicted based on nucleotide sequence

MQCPPILLVWTLWIMAVDCSRPKVFLPIQPEQEPLQSKTPAGCTFGGKFYSLEDSWHPDL
GEPFGVMHCVLCYCEPQRSRRGKPSGKVSCKNIKHDCPSPSCANPILLPGHCCKTCPKAP
PPPIKKSDFVFDGFEYFQEKDDDLYNDRSYLSSDDVAVEESRSEYVALLTAPSHVWPPVT
SGVAKARFNLQRSNLLFSITYKWIDRLSRIRFSDLDGSVLFEHPVHRMGSPRDDTICGIW
RSLNRSTLRLLRMGHILVSLVTTTLSEPEISGKIVKHKALFSESFSALLTPEDSDETGGG
GLAMLTLSDVDDNLHFILMLRGLSGEEGDQIPILVQISHQNHVIRELYANISAQEQDFAE
VLPDLSSREMLWLAQGQLEISVQTEGRRPQSMSGIITVRKSCDTLQSVLSGGDALNPTKT
GAVGSASITLHENGTLEYQIQIAGTMSTVTAVTLETKPRRKTKRNILHDMSKDYHDGRVW
GYWIDANARDLHMLLQSELFLNVATKDFQEGELRGQITPLLYSGLWARYEKLPVPLAGQF
VSPPIRTGSAGHAWVSLDEHCHLHYQIVVTGLGKAEDAALNAHLHGFAELGEVGESSPGH
KRLLKGFYGSEAQGSVKDLDLELLGHLSRGTAFIQVSTKLNPRGEIRGQIHIPNSCESGG
VSLTPEEPEYEYEIYEEGRQRDPDDLRKDPRACSFEGQLRAHGSRWAPDYDRKCSVCSCQ
KRTVICDPIVCPPLNCSQPVHLPDQCCPVCEEKKEMREVKKPERARTSEGCFFDGDRSWK
AAGTRWHPFVPPFGLIKCAICTCKGSTGEVHCEKVTCPKLSCTNPIRANPSDCCKQCPEE
RSPMELADSMQSDGAGSCRFGRHWYPNHERWHPTVPPFGEMKCVTCTCAEGITQCRRQEC
TGTTCGTGSKRDRCCTKCKDANQDEDEKVKSDETRTPWSF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable