Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACGCCCAAAAAGTAGCAGCTC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCAGCCCCCTCCTCC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710459
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4758nts
Open reading frame: 1 to 4758
ATGCAGCCCCCTCCTCCGCAAGCAGGGCCTCCTGGCACGGGCGGTCCTCCCCGCATGGGA
ATGGGCAATAATGGCTACTGGCGCAAAAGGGGACCCAATCCTGTACCAGCTGCTTCAGCT
TCAGTGCAACCAGTAACAGACCCTTTTGCCTTTGGCCGGATGACCCCACAAAGTATGGCA
CAGGGAAGTGGCCCGATAGGAAACCCACCGAGTGTTTCCCCTTCGCTGTACCCTCAGCCC
ATACCTGTACATGCATCGGGTGCAGGAGATCAAGGACATGGGTCACTACCAGGATCTGTA
CCACAACCTGCTGCTATGGGGGGGATGTATACAAATGTATCTGCCCCATCACCAGCAAGC
GCCCCCTTTCCCAACTACCCAGCCGCCACAGTTAATGCTCAGCCTGGACCCAGTAACATG
CAGACTTTTCAGAATACAGCGAGTGGACTTGAAAATGCCTTTCTGCCAGTGCAAAGCAGG
CCTCAGTCGACACAAAGCATCAGTTATAACCCTCCATCTGCTTCACCTTTACAGCACTTT
GTCTCTGCACCATCTCAGCCAACTACTGGTTCCTGGGGGTCCTTACAGAGTGCACCTCCT
GTCTCAGCTCAGAACTACCCTCTTCCTGTTCAACAACCATCCCATAATGCTCCTTATTAT
TCCCCACCTTATTTAGAAGTGTCTAATCTGGCCCCTCGAGCCAATGAAACTCCTGGGCAT
TACCCACCAACCACCGTCTCCAATTCTACCTTTCAGCACAGCTATTTACAACCCACCGCT
TTAAATATGACAGACTATATGCATAGCCACAATCCCGAGCAGAACTGGTTTAACCAACCT
TACAAGGATCAACGGCATCCACCTCTCCCCAGTCCTAACCCCGTACAATACCCAACTCAG
CCACCCGAACCTCATCAGGAGGCGACTAATGCATCTGCTCATGCCTCCTCCAATGCAGAC
TCTGGTACTATATCAATGTTTTTTAAAGGGGGCGAAGAAGAAAATGTAGAAATCGTTTCT
TCGGACCAGCAGAATGCATCTGTTAAATTTGATCATGGCTCTTCTCATTATCAGAACGCT
GGACATCAAGGTGTGCCTTCTCTCCTGCCGCAGCCAACAGTCCCGCCACTTCCACCCATC
TCTTTACCATCTGGCGTTCCCCATGTCCCCGCGTCTGTGTCTCAGAATGTTGGAAATGTG
ACCCATCCGCATTCAGCTTGGCGACCGGACGGGTTGACTGTTAACGATAAAACCAATAAA
CCCAATGCTGGAAGTCAATTGCCCTATGACAATGCTGAAAATGTAGAGTGTGTCCAAAAC
CAGGAAGTCCCCCCCAATGAGCCCAGTCATTTTAACCTTTATCCTTCTAATCTAAATTCA
GATATGCACCGATATGGGTCGATGCCTGGACCCTCTCTACCAAAAAATGTTGGCAGCCAA
CCTGAAGCAGGTCCTAACATAGAGACACCGGAAACAGTGGGTTGTCCTGTGAGATCCGAT
AGCGTTTCTTCAAATTATAGCAACCTGAGTCACAAAAGCCTACCTGTGCCCACCCGACCT
CCAGAGCTGGCCGGGACTTTTATTCAGAAGGAGAGCGGGAGGCTTGAAGGTGACTCTTCC
CACGGTTTCTTTAAGCAAATCGATTCATCTCCTATGGTGGGTGATGCTTTAGGGCAGAAT
ATGGGTCAGCAGACGCATCACAGCAATTTATCCCAACCACCGACTCCAAGTCCTCCTAAA
CCTACTGGGATCTTCATGACGAGTGCAAATAGTTCTTTCGAACCAGTAAGATCACACAGC
ATTGGTTTGAAAGCGCCAGACATTGGCCAATTGAAAATGGGCGGAGACGTAAGAGAGGGG
CATAAGGTTCCACCAAACGCCTCTGTCCCGGCAGCTTTCCCTGGTAACTTGGAACAGCCT
CCGGATAATCTGGAAACCATATTCTTAGGTAAAAGAAATTCTCATGGAACCATTACCAGC
AACGGGAACCTGTTCCAATCTGTTGGGGAACCGCCATTGGACAATATATCACTACTGCTT
GACAAACGTCCATCGTCAAGGGCACACGGGTCTAACAAGAAATGCGAAAGCCCAGCAACC
ACCCTGTGGGCTCAAGGAGAGCTTCCAAATTTTGGAGGAAATGTTCTGTTGGCTCCTGCG
GCTCCTTCACTCTATGTGCCCCCTAAAACCCAAACTGAAATAATTCAGCCCCCTGAAGAT
CGGTCTTATGATTCACGCCCAGATCTGGGGACTTTTCAGGCCTCTCCAGAAGAACAGATT
TCCTCTGAAAACCTTGAGAATCCCCCAAAGATCGGAGATGAGGAGCAGCTTCCCTCGCAG
GCAAGTTCAGGTTATGCCAGTTTGTTATCTTCGCCGCCCACTGAGGCGCTGCAGAATCAG
CCCTTGCTAATCGCCCAACCAAATCAAACCTATAATTTGTCGCAGCCTATTAATTTTTCG
TTGTCGTTACCAAATCAGGTGAAAAACATTAATTCCTTTAAGGAAACGGGAGCAGCCAAT
GACAGTACGGTGTTTTCCCTGGCTCAATGCCGACCTGAGGCTTCTAACCCACCTAATTAC
AAATCTGCAGCTCCTAATGATAAATTCTCTGAATCTCTTCCCAATCAGAATTCCGTTATG
CAGTGGCAATCATCAGCCAATCAAGCGCCCCTCAATTTAGCCGCGGAAAGCCAAAAGGCA
CAGAAGGTTGAAAACGCAACTGCTGACCTTGATAATAAAAACACAATTCCGACGACCGCT
GCTCCGACGGGGCCACGTTTCCCTGCCAATCCTAACGGCCGGCCCAGTTATCAAGCAGAG
AAGACTGGCGCTTTAGATTTTACAGTCCCTCGTCCGCCGGTCTGTTCTGTTGCCAGTAAC
ATTGCTGCAGGTAACAGTCAGCCAGTTGCTGGAGGGCCGCTCTTTCCTGGGCAGTCTGTC
CGCTCTCAGAATGGTCAGGCAGGCTTAGATGTTAACAAGCAGGGTTTTTATCAGCAGGTC
ACTAAGGATGGGCCCCCGCAGCAGCCTGCTATGGATATTCCACATCAAGGAGGAGGGGCC
CCGCAGGAATACATGGTCCCTTCATCTCAGCCGCTCTCCTTGGTGCAGCCTGCACAAGCA
CCTCCAGGAGGTAAACCAATTCCATACCCTCCACAAGCTGAAAGCCAAGTTCCAACATCA
CAGAACCCCAACAGCACTCCTTATAACACATACGGACCAACTGAACAGCCTCCCTATCCA
GTCCAGCCTGTTCCTTCCTCATCAGCTCCAGTGAATCTCAATAACACAGCAACATATCAA
GAGCAGCCCAGGCCTCCTGCCTCCCATACCCCTCAACAAGCTGATCCTTATTATTACTAC
AGGTCTTATGGTGCCTACCAACAGCCATACCCGCAGCCTTACCAACCCCTGGATCCAAGG
GCAGCGCCTCTTTACTATCCAGACCCCTATGCAGCTTATGACCCCACCTATAGGCAGTAC
AGCGGGTCTTCATATGCGAACCCTGCCAAGTACCCCTACCAGGAGCCCGAACGTCCCAGT
TCTAGATCCAGCCACTCTTCTGATCGTCCCTCCTCAAGGCAGGGTTATAGCAGTAAAGGA
GACTGGAATGCACAAAGTGGTTACTACCCGGGGTATTATCAGAATCAATATGATTATGCA
GACCCTACCCGCTGGGACCGCTATCCCGGAGCCTATGACCCTGGCTATAGAGATCCCAGG
AGTAATTACTGGAATTATATGTACAACAGCCGTGATGATGCGTACCAAAGGAACGTACCC
GACGCTTATACCAGCAGATATGACGTGTATGAAGACCGCTGGCAGTATGACCCACGTTAT
GTGGGCGGCTTTGATGACGAGCCGCGGAGAGACCCCACAAAGGACGACTTTGACCGGCGC
AGTGTTCACAGCGAGCATTCAGTCCATAGCATGCACAGCGGCCAGAGCTCACACAGCCGG
AGAAGCAGTTTCAGCTCCCATTCCCAACTTTCCCAACATAGCCAGATCTACAAAAGCCAG
CCAGACCTGACTGCCAGCGCCTATGAACCCCAAATTCAGAATCCTCCGCTCAATGAGTAT
TCATATGGCATGTACCCCAATGACTACAGCACCCAGCAGGCGCTTGATAATTACTCTTAC
GGGTACACCAAGGGCACGGAGTGGCAACCTGTAGAGCAAGTGCCTTCAAGGCCATTGACT
CCAGAGAAGTTTGGGAGCCCACATATCTGTGCCAGGTTTGGTCCAGGGGGTCAGTTAATT
AAGGTGCTGCCTAACCTTCCTTCAGATGGGCAGCCGGCCTTAGTGGAAATCCATAACATG
GAGGTCATTATGCAGAACAGCCCAGAGCAGGAACAGCTGAGAAGTTTCCCCGGACCTTTA
GTGAAGGATGAAACTCACAAAGTCGACGTCATCAATTTTGCTCAGAAGAAATCCAAGGAG
TGTTTTCAGAACGAGAGCCTGCTAGACCAAGAGTCCTCTTGCCTGCTCTGGGACTTCATT
GTGCTTCTGTGCCGGCAAAACGGGACTGTTGTGGGAACAGATATCGCCGAGCTCCTTTTG
AGAGATCACAAGACCGTGTGGCTTCCTGGAAAGTCTCCCAACGAAGCCAACCTGATCGAT
TTCAGCAACGAGCCGCTGGAGCAGCAGCAGGAGGAGTCGGGGGCTTCTCAGTTGTCCTTC
CTCACGGATACCTTGCAAAGCACCGGGGAAGCTCTACAAGGAGAACGAGCGGTTCAGAGA
GCTGCTACTTTTTGGGCGTAA
Protein sequence predicted based on nucleotide sequence
MQPPPPQAGPPGTGGPPRMGMGNNGYWRKRGPNPVPAASASVQPVTDPFAFGRMTPQSMA QGSGPIGNPPSVSPSLYPQPIPVHASGAGDQGHGSLPGSVPQPAAMGGMYTNVSAPSPAS APFPNYPAATVNAQPGPSNMQTFQNTASGLENAFLPVQSRPQSTQSISYNPPSASPLQHF VSAPSQPTTGSWGSLQSAPPVSAQNYPLPVQQPSHNAPYYSPPYLEVSNLAPRANETPGH YPPTTVSNSTFQHSYLQPTALNMTDYMHSHNPEQNWFNQPYKDQRHPPLPSPNPVQYPTQ PPEPHQEATNASAHASSNADSGTISMFFKGGEEENVEIVSSDQQNASVKFDHGSSHYQNA GHQGVPSLLPQPTVPPLPPISLPSGVPHVPASVSQNVGNVTHPHSAWRPDGLTVNDKTNK PNAGSQLPYDNAENVECVQNQEVPPNEPSHFNLYPSNLNSDMHRYGSMPGPSLPKNVGSQ PEAGPNIETPETVGCPVRSDSVSSNYSNLSHKSLPVPTRPPELAGTFIQKESGRLEGDSS HGFFKQIDSSPMVGDALGQNMGQQTHHSNLSQPPTPSPPKPTGIFMTSANSSFEPVRSHS IGLKAPDIGQLKMGGDVREGHKVPPNASVPAAFPGNLEQPPDNLETIFLGKRNSHGTITS NGNLFQSVGEPPLDNISLLLDKRPSSRAHGSNKKCESPATTLWAQGELPNFGGNVLLAPA APSLYVPPKTQTEIIQPPEDRSYDSRPDLGTFQASPEEQISSENLENPPKIGDEEQLPSQ ASSGYASLLSSPPTEALQNQPLLIAQPNQTYNLSQPINFSLSLPNQVKNINSFKETGAAN DSTVFSLAQCRPEASNPPNYKSAAPNDKFSESLPNQNSVMQWQSSANQAPLNLAAESQKA QKVENATADLDNKNTIPTTAAPTGPRFPANPNGRPSYQAEKTGALDFTVPRPPVCSVASN IAAGNSQPVAGGPLFPGQSVRSQNGQAGLDVNKQGFYQQVTKDGPPQQPAMDIPHQGGGA PQEYMVPSSQPLSLVQPAQAPPGGKPIPYPPQAESQVPTSQNPNSTPYNTYGPTEQPPYP VQPVPSSSAPVNLNNTATYQEQPRPPASHTPQQADPYYYYRSYGAYQQPYPQPYQPLDPR AAPLYYPDPYAAYDPTYRQYSGSSYANPAKYPYQEPERPSSRSSHSSDRPSSRQGYSSKG DWNAQSGYYPGYYQNQYDYADPTRWDRYPGAYDPGYRDPRSNYWNYMYNSRDDAYQRNVP DAYTSRYDVYEDRWQYDPRYVGGFDDEPRRDPTKDDFDRRSVHSEHSVHSMHSGQSSHSR RSSFSSHSQLSQHSQIYKSQPDLTASAYEPQIQNPPLNEYSYGMYPNDYSTQQALDNYSY GYTKGTEWQPVEQVPSRPLTPEKFGSPHICARFGPGGQLIKVLPNLPSDGQPALVEIHNM EVIMQNSPEQEQLRSFPGPLVKDETHKVDVINFAQKKSKECFQNESLLDQESSCLLWDFI VLLCRQNGTVVGTDIAELLLRDHKTVWLPGKSPNEANLIDFSNEPLEQQQEESGASQLSF LTDTLQSTGEALQGERAVQRAATFWA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
