smarcd2 (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGTGAGACGGAGCCCCAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGATGCGGGGCCCAAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710648
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1395nts
Open reading frame: 1 to 1395

ATGATGCGGGGCCCAAACCCTGGAGTCTACAGACCCCCCATGGGGCCCTACCAGAGACCA
GGAATGCTTCCAGGCGCCCGAATGCCAATGGCCGGCATGCATATGGGCTCCCCCATGCTT
CCTCCGTACGGTGGGGGTTCTCCCATGAGACCTGCCATGAATCCAATGATTATGGAGCCG
TTTAGGAAGAGACTGCTCACCCCACCGGGACACCCCCATGGGATGAGCCAGCGTAGAGGA
GTTAAGCGAAGAAAGATGGCCGACAAGATCTTGCCCCAGAGGATCCGTGAACTGGTGCCG
GAGTCTCAGGCCTACATGGACCTCTTGGCATTTGAGAGGAAGCTGGATCAGACTATAGCG
AGGAAGAGGATGGAGATTCAGGAGGCCATCAAGAAGCCCCTCACGCAAAAGCGCAAGCTG
AGGATTTACATCTCCAACACGTTCACTCCAGCCAAGCCCGACGGGGAAGAGGCGGAAAGA
GTCAGCTCTTGGGAGCTCCGAGTGGAAGGGAAGCTTCTGGAAGACCCCAGCAAACTGAAG
AGGAAGTTCTCCTCCTTCTTCAAGAGTCTGGTCATTGAGCTGGATAAGGACCTGTATGGG
CCGGACAATCATCTGGTGGAGTGGCACAGGATGGCGACCACGCAGGAGACGGATGGATTT
CAGGTGAAGCGCCCTGGAGATGTGAATGTGAAGTGCACCTTGCTCTTGATGCTAGACCAC
CAGCCCCCTCAATATAAGTTGGACCCTCGTCTCGCACGGCTGCTGGGTGTTCACACTCAG
ACCCGGGCCAACATCATGCAGGCCCTCTGGCTCTACATCAAGACCAATAAACTGCAGGAC
AGCCACGAGAAGGAGCACATCAACTGCAACCGCTACTTCAGACAGATTTTCAACTGCTTG
CGCATGAAGTTCTCCGACCTACCCATGAAGTTGGCGAGTTTGTTGCAGCACCCAGACCCC
ATCATAATTAATCATTCCATCAGTGTGGACCCCAATGACCAGAAGAAGACTGCTTGTTAC
GATATAGAAGTTGAGGTCGACGACCCTCTGAAGGTGCAGATGAGCAGTTTTCTCGCTTCC
ACCACCAACCAGCAAGAGATTGCCAACCTGGATTCCAAGATCCATGAGACCATTGAGTCC
ATCAATCAGCTGAAGACGCAGAGAGATTTCATGTTGAGCTTCAGCAATGACCCCCAAGAC
TTTATTCAGGACTGGCTGAAATCCCAAAGCAGGGACCTCAAGATCATGACGGACACATTG
GGAAATCCCGAGGAGGAGAGACACACGGAGTTCTATCAGCAGCCGTGGGCCCAGGAGGCA
GTGGGGAGACACATCTTCTTTAAGGTGCAGCAACGGCGGCAAGAACTGGAGCAGGTTTTG
GGGCTCCGTCTCACCTAA

Protein sequence predicted based on nucleotide sequence

MMRGPNPGVYRPPMGPYQRPGMLPGARMPMAGMHMGSPMLPPYGGGSPMRPAMNPMIMEP
FRKRLLTPPGHPHGMSQRRGVKRRKMADKILPQRIRELVPESQAYMDLLAFERKLDQTIA
RKRMEIQEAIKKPLTQKRKLRIYISNTFTPAKPDGEEAERVSSWELRVEGKLLEDPSKLK
RKFSSFFKSLVIELDKDLYGPDNHLVEWHRMATTQETDGFQVKRPGDVNVKCTLLLMLDH
QPPQYKLDPRLARLLGVHTQTRANIMQALWLYIKTNKLQDSHEKEHINCNRYFRQIFNCL
RMKFSDLPMKLASLLQHPDPIIINHSISVDPNDQKKTACYDIEVEVDDPLKVQMSSFLAS
TTNQQEIANLDSKIHETIESINQLKTQRDFMLSFSNDPQDFIQDWLKSQSRDLKIMTDTL
GNPEEERHTEFYQQPWAQEAVGRHIFFKVQQRRQELEQVLGLRLT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable