sphk1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATGATGGCTGCGGGATACT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGTGATACCCAAGATTC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710910
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1527nts
Open reading frame: 1 to 1527

ATGGGTGATACCCAAGATTCCTCAAGTATCCCCTCTGAGGTTATACTCTGTGACACTTTC
TCCCTTGTACCAGGCACTGGCTCTCCCTTGTCACTCATCCTCACCCACAAGGAGCTTCTG
ATTCAGAATCTGTCCCTGGACGCTCAGCTGTCTCCCCAATACTCCCTTGCCCTGACCGAC
TGCATCGGATGCCGCTCCTTCAGAGGAAAAGACCCAACAGACCTTGGGGGTTATTTTACT
GCAGTGTTTTACCCCCTTAGAGGCAAGGCGCTGGGGACAACAGTTTATCGGCAGAGAGTT
TCCAGGACGTTCAGAGCAGAAGGTTCAAGAGACAAAGAGCAGAACTTTAATGTTGCTCAG
AGCTGGGCCAAGAAAATATGGGAAATGGCTGAAGAGCAAGAGCATCCTGGCAACCCTCTG
CCTTCCTCATGTCGCTTTCTGGTACTACTAAATCCATTTGCGGGAACCGGAAAAGCCTCA
GCCCTCTTCCAAACACACGTGATTCCCATGTTAACGGAGGCCAATGCCACCTTCACTCTC
CTAGAGACAGAGAGGCCCAAGCAAGCGTATGAGTTGGTGCGTGATGAGGACCTGTCCGGC
TGGGATGCCATCGTTGTGATGTCAGGGGATGGGCTGGTGTTTGAGGTTATTAATGGTTTG
ATGGAGAGGCCGGACTGGGTCTGCGCCATTAAGAAGCCGATTGCTGTTCTACCAGGTGGA
TCTGGGAATGCTCTGGCTGCATCTATCAGTTACTATTCTGGGCACAAACAAGCGGTGGGC
AACAAGCTGCTGAACAATTGTACATTCATCCTGTGCAAGGGACAGCCTGTACCCCTGGAT
CTTGTATCTTTTACCACCTCCTCTGGAAGGCGTATCTTCTCTTTCCTCAGTTTTGCTTGG
GGTTTAATCTCTGACGTGGATATTGAGAGTGAGAGGTACAGGTTTATGGGTTCTGCCCGT
TTCTCATTTGGCACTTTTGTCAGACTGACGGCACTACGTACCTATAGAGGGCGCCTCTCG
TACCAAGCCGCTAAAACTTCAGTGGACACTGCACCAGAGAGTGACTCACATAGGCGCACC
TTAAATGATTCTACAGATATTGTGAACCCCCATGTTTTAGAGGACTCATTGCTTGTCCCA
TTGAATGAGCCAGTTCCACCTCACTGGACAACTGTTATGGAGGACCAGTTTGTGCTTGTC
CTGTTACTCTACCAGTCCCACCTTGGGGCTGACCTCTTCACTGCTCCGATGGTGCAGAGC
CCAGGAGAAGGGGTCATGCAGCTGTTCTACGCCACCTCTCGAGTCTCCCGGGCATCACTT
CTTAAACTCTTCCTTGCCATGGAGAAAGGGACTCACTTACATGAGTCCATCCCTCACATA
ATCAATGTCCCTGTTACAGCATTTCGGGTGGAACCATTTGAGAGCAATGGGATCATGACT
GTGGATGGAGAGGCTATACCATGTTGTTCCCTCCAGGGTCAGATACATAAAGGTCTTGGA
CGCATCATAAGTATCCCGCAGCCATCATGA

Protein sequence predicted based on nucleotide sequence

MGDTQDSSSIPSEVILCDTFSLVPGTGSPLSLILTHKELLIQNLSLDAQLSPQYSLALTD
CIGCRSFRGKDPTDLGGYFTAVFYPLRGKALGTTVYRQRVSRTFRAEGSRDKEQNFNVAQ
SWAKKIWEMAEEQEHPGNPLPSSCRFLVLLNPFAGTGKASALFQTHVIPMLTEANATFTL
LETERPKQAYELVRDEDLSGWDAIVVMSGDGLVFEVINGLMERPDWVCAIKKPIAVLPGG
SGNALAASISYYSGHKQAVGNKLLNNCTFILCKGQPVPLDLVSFTTSSGRRIFSFLSFAW
GLISDVDIESERYRFMGSARFSFGTFVRLTALRTYRGRLSYQAAKTSVDTAPESDSHRRT
LNDSTDIVNPHVLEDSLLVPLNEPVPPHWTTVMEDQFVLVLLLYQSHLGADLFTAPMVQS
PGEGVMQLFYATSRVSRASLLKLFLAMEKGTHLHESIPHIINVPVTAFRVEPFESNGIMT
VDGEAIPCCSLQGQIHKGLGRIISIPQPS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable