ttc30a (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACAAATTCCATGAGGTTATCTCATA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTGTAAGGCAGATTAAAGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711049
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1953nts
Open reading frame: 1 to 1953

ATGGCTGTAAGGCAGATTAAAGATGGAGAATATACCGCGACTATCTACAGACTGATAAAG
GAAGCCAGATATGGAGAAGCTATCCAGGTTCTTAGCAATGAACTTCAGAAGCAATATAGG
TCTAGAGCTGGCCTTTCCCTCCTGGGCTACTGCTATTATCAGATCCAAGATTTTGTGAAT
GCTGCAGACTGCTATGAACAGCTGATTCAGATCTCTCCTGAAGTGGAAGAATATAAATTG
TACTATGCACAGTCCCTGTATAAGGCTTGCATGTATCCAGAAGCAATGAAGGCAACGTTT
GCTTTAGACAACGCTGCCTATCAAAGCAAGATGTTGAAATTACAAGCTTCTGTCAAATAT
GGAGAAGAAGACATTTCAGGAGCTAAGAGTTTAGTAGAGCAGATGCCATCAGAAGACCCT
GAAAGTGAGATTAATATGGGGTGTTTACTGTATAAGGAAGGACATTATGAAGAAGCCTGC
AAAAAGTTCATTACTGCAATGCAAGTCATGGGCTATAAACAAGATTTATCTTTCAACATT
GCATTGTGTTACTATAGCATGAAACAGTATGCTCCTGCCTTAAAGCACATAGCTGATATA
ATTGAACGAGGAATCCGAGAGCATCCTGAGCTTGGTGTTGGCATGACAACAGAAGGCATT
GAAGTTCGAAGTGTGGGGAACACTCTTGTACTTCATGAAACTGCACTCATAGAAGCATTT
AACCTTAAAGCTGCTATTGAATACCAGTTAAAGAATTATGAAGCAGCTCAAGAAGCCCTC
ACAGACATGCCCCCAAGATCTGAAGAGGAACTTGATCCAGTTACTCTTCATAATCAAGCC
TTGATGAATATGGATACTAAACCCACTGAAGGGTTTGAGAAACTGCAGTTCTTACTACAG
CAGAATCCTTTCCCCCCAGAAACCTTTGGCAATCTCTTACTTTTATACAGCAAATATGAG
TATTTTGATCTTGCAGCAGATGTTCTGGCTGAAAATGCTCATCTCACCTATAAATTTCTC
ACACCATATTTGTACGATTTTCTGGATGCCATGATAACCTGCCAGACTGCACCTGAAGAG
GCTTTTCTCAAGCTTGATGAACTAGCTGGAATGCTTACGGAACAAATGAGAAAGTTAACA
AAACAGGTTCAAGAGGCACGGCATAACAGGGACGATGAGGCTGTTAAGAAAGCTGTAAAT
GAATATGATGAAACCTTGGAAAAGTACATTCCAGTATTGATGGCACAGGCTAAAATTTAT
TGGAACATGGAGAACTACCAGATGGTGGAAAAAATTTTCCGTAAATCAGTGGAGTTTTGC
AATGAGCATGATATCTGGAAGCTGAATGTGGCTCATGTTCTCTTCATGCAAGATAACAAA
TACAAGGAGGCTATTGGCTTTTATGAGCCCATTGTCAAAAAACACTATGATAATATATTA
AATGTCAGTGCAGCTGTTCTGGCTAATCTGTGTGTTTCCTACATCATGACGAGTCAAAAT
GAAGAGGCTGAAGAACTCATGCGCAAGATTGAAAAGGAGGAGGAACAGATTGCTTATGAA
AACCCTGATAAAAAGATCTACCATCTTTGTATTGTCAATCTAGTCATTGGAACACTTTAC
TGTGCAAAGGGGAATTATGAGTTTGGCATTTCTCGAGTTATCAAAAGTTTGGAGCCATAC
AACAAAAAGCTGGGAACAGACACATGGTATCACGCAAAGAGATGCTTCCTTTCGTTGCTG
GAAAATATGTCAAAGCACATGATCATGCTGCGTGATGACGTTATAGTGGAATGCATACAG
TTCTTGGAGCATTGTGAAATTTATGGCAGAAACATTCTTGCTGTAATTGAGCAACCACTA
GAAGAAGAGAGAATGCACATCGGAAAGAATACAGTCACCTATGAATCCAGGCAGCTTAAA
GCTTTACTCTATGAGATAACCTCATGGAATTTGTAA

Protein sequence predicted based on nucleotide sequence

MAVRQIKDGEYTATIYRLIKEARYGEAIQVLSNELQKQYRSRAGLSLLGYCYYQIQDFVN
AADCYEQLIQISPEVEEYKLYYAQSLYKACMYPEAMKATFALDNAAYQSKMLKLQASVKY
GEEDISGAKSLVEQMPSEDPESEINMGCLLYKEGHYEEACKKFITAMQVMGYKQDLSFNI
ALCYYSMKQYAPALKHIADIIERGIREHPELGVGMTTEGIEVRSVGNTLVLHETALIEAF
NLKAAIEYQLKNYEAAQEALTDMPPRSEEELDPVTLHNQALMNMDTKPTEGFEKLQFLLQ
QNPFPPETFGNLLLLYSKYEYFDLAADVLAENAHLTYKFLTPYLYDFLDAMITCQTAPEE
AFLKLDELAGMLTEQMRKLTKQVQEARHNRDDEAVKKAVNEYDETLEKYIPVLMAQAKIY
WNMENYQMVEKIFRKSVEFCNEHDIWKLNVAHVLFMQDNKYKEAIGFYEPIVKKHYDNIL
NVSAAVLANLCVSYIMTSQNEEAEELMRKIEKEEEQIAYENPDKKIYHLCIVNLVIGTLY
CAKGNYEFGISRVIKSLEPYNKKLGTDTWYHAKRCFLSLLENMSKHMIMLRDDVIVECIQ
FLEHCEIYGRNILAVIEQPLEEERMHIGKNTVTYESRQLKALLYEITSWNL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable