phkg2 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACATCTCCAAGTCCCGCTCA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACACGGGACGCCGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711268
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1185nts
Open reading frame: 1 to 1185

ATGACACGGGACGCCGAGGCACGGGATGAACTCCCTGACTGGGCCGGTGCCAAGGAATTC
TACCAGAAATATGATCCCAAGGAAATCATCGGCAGGGGGGTTTCAAGCACGGTACGACGG
TGTATTCATCGAGAGACGGGCCGGCAGTTTGCCGTGAAGATTATTGAAGTGACTCCGGAG
AGACTGAGTCCTGAGCAGCTGAAGGAGGTGCGACTCTCTACTGCCAAGGAGATGGAGATT
CTGCACCATGTCTCTAACCACCCTTCTATCATTTCACTGATAGACTCCTATGAATCCTCC
ACGTTTATATTCCTGGTGTTTGACTTGATGAAGAGAGGGGAACTGTTTGATTACCTGACG
GAGAAAGTCACACTCAGTGAGAAAGAGACCAGGTGTATTATGAGGTCTCTCTTGGAAGCA
GTTTATTACCTGCATACGAACAACATCGTGCACAGGGATCTCAAGCCCGAGAACATTCTC
ATGGATGACTGTCTCAACATCAAGCTCTCGGATTTTGGCTTTAGCTGCATCCTAAAGCCT
AATGAGAAGCTACGAGAGCTTTGTGGGACACCTGGGTATTTGGCTCCAGAGATATTGAAA
TGCTCAATGGATGAGACCCACCCAGGATACGGCAAAGAAGTTGATCTCTGGGCGTGTGGA
GTGATTGTGTTCACACTGTTGGCCGGATCACCCCCTTTCTGGCACAGGAGACAGATGCTG
ATGCTCCGTATGATTATGGATGGTCGATTTCAGTTTGGTTCTCCGGAATGGGACGACCGA
TCTTCCACTGCTAAAGATCTGATCTCTCGTTTATTGGAGGTCTGTCCCGAGAAGCGTCTC
ACGGCAGAGCAGGCTCTCCAGCATTCTTTCTTCCAGAGCCACCAGAGAGACCAGGATACT
CCTGTCAAACGATCTCAACGCTTCCGGATTGCAGCCTGGGCTGCACTGGCGTGTGTCCGT
ATCCTGGTACGATGGAGGCGAGCCCGGCCTATCACAAGTCAGCTTCTGATGAGGGACCCA
TACGGAATTAAAGGCGTCCGTAGACTGATTGATGCGTGTGCTTTCCGCATTTATGGGCAC
TGGGTGAAAAAGGGAGAGAGACAGAACCGGGCAGCGTTATTTCAGAACCAGCCCAAAGCC
CTGTTACTTGCTATGGGGGATGATGATGAGCGGGACTTGGAGATGTGA

Protein sequence predicted based on nucleotide sequence

MTRDAEARDELPDWAGAKEFYQKYDPKEIIGRGVSSTVRRCIHRETGRQFAVKIIEVTPE
RLSPEQLKEVRLSTAKEMEILHHVSNHPSIISLIDSYESSTFIFLVFDLMKRGELFDYLT
EKVTLSEKETRCIMRSLLEAVYYLHTNNIVHRDLKPENILMDDCLNIKLSDFGFSCILKP
NEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVIVFTLLAGSPPFWHRRQML
MLRMIMDGRFQFGSPEWDDRSSTAKDLISRLLEVCPEKRLTAEQALQHSFFQSHQRDQDT
PVKRSQRFRIAAWAALACVRILVRWRRARPITSQLLMRDPYGIKGVRRLIDACAFRIYGH
WVKKGERQNRAALFQNQPKALLLAMGDDDERDLEM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable