Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGCGTGATCAGACTTTGCTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTGCGACTCCGGC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711375
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6303nts
Open reading frame: 1 to 6303
ATGGCTGCGACTCCGGCCCTATCGACAGCAGGTGCTTTACAGCCTCGCTGGAAGAGAGTA
GTTGGATGGTCAGGCCCTGTGCCTAGGCCGAGGCATGGACACAGAGCTGTGGCTATTAAG
GAACTTATTGTGGTTTTTGGAGGAGGAAACGAGGGCATCGTGGACGAGCTGCATGTTTAT
AACACGTCCACAAACCAGTGGTTTATTCCAGCTGTGAGAGGTGACATACCGCCTGGATGT
GCCGCCTATGGATTTGTCTGTGATGGGACACGTTTACTTGTGTTTGGAGGAATGGTGGAG
TATGGGAAGTACAGCAATGACCTATATGAGTTGCAGGCAAGCAGATGGGAATGGAAAAGA
CTTAAAGCCAAAGCACCCAAGAATGGTCCACCGCCATGTCCTCGTCTTGGACACAGTTTC
TCATTGGTTGGAAGCAAATGCTATCTTTTTGGTGGGCTTGCTAATGATAGTGAGGACCCC
AAGAACAACATTCCTAGGTACCTTAATGATCTTTACATCTTGGAGTTGCGCGCTGGCTCT
GGAGTTGTAGCTTGGGATGTTCCTATAACATATGGTATTCTGCCGCCACCTCGTGAGTCC
CACACTGCAGTGGTTTACACGGACAAAGATAACAAGAAGTCCCGTCTGGTAATATACGGG
GGTATGAGTGGATGCAGACTTGGAGATCTTTGGATTTTGGATATAGATACTCTGACCTGG
AGCAAACCAAGTCTGAATGGTGTTGCACCTCTCCCAAGAAGTCTTCATTCTGCCACAACT
ATATTGAATAAAATGTATGTCTTTGGAGGATGGGTGCCTCTTGTAATGGATGATGTTAAG
GTAGCTACCCATGAGAAAGAATGGAAATGCACTAATACACTTGCGTGCCTCAACCTTGAG
AGTATGTCATGGGAGCATATAGTAATAGACACCTTAGAGGATAACATTCCACGTGCTCGT
GCAGGCCATTGTGCAGTGGCCATCAATACACGTCTTTATATTTGGAGTGGTCGAGATGGT
TACCGCAAAGCCTGGAACAACCAGGTTTGTTGCAAGGACCTGTGGTACCTTGAAACAGAG
AAACCACCTGCACCTGCTCGTGTACAACTGGTAAGAGCAAACACCAATTCCCTGGAGGTT
AGCTGGGGGGCTGTGCCCACTGCAGACAGTTACTTATTGCAGCTACAAAAGTATGACATA
CCAGCTGCTGCTGCAGCAATGTCTCCAGCCAATCCAGTTCCATCTGTGCCAGTGAATCCA
CCCAAGAGCCCTGCACCTGCATCTGCAGCTCCAACAGTACAGCAGGTTGCTCACACTGGT
ATCACCCTTTTACCGCAAGCAGCCTCTTTACCTCCCACAACAGCCACAATCCAGGTCTTG
CCTACTATGCCTGGGAGTCCTATTGCAGTGTCTGCTGCTCTTCGCACACAAGCTGTCCCA
GCTGTCCTTAAAGTTGGAGGCCAGCAGTCCACTCTGAGCACGCCTCTAGTTACTGTAAGG
CCAGCTAATCAGATTAGTAAAGCTCCTGTTACAGTCACTTCTCTCCCGCCTGGTGTGCGT
ATGGTTGTTCCTGCACAGAGCACCCAGGGAGCTCCTATTGGCAGTAGTCAGCCAATGAGT
GGCATGGCTGCTCTTGCTGCAGCTGCTGCTGCAACACAGAAGATACCCCCTTCCTCTGCA
CCCACCATGCTTGGTGTCCCTGCTGGAGCTACTATGGTGAAATCTGTGGCTGTGCCCTCT
GGAGTCACCTCACTTCCCACTACTGTCAAAGTGGCATCCTCTCCAATAATGGTCAGCAAC
CCAGCAACCCGTATGTTGAAAACAGCCGCTGCACAAGTTGGAACTTCAGTTCCTGGTAGC
AATACACAGAATCGTCCAATAATCACGGTGCATAAGTCAGGAACTGTGACTGTTGCCCAA
CAAGCCCAGGTTGTAACCACTGTGGTGGGCGGTGTTACCAAAACCATCACTCTAGTTAAG
AGTCCCATCTCTGTTCCTGGTGGCAGTGCTTTGATATCCAATCTTGGGAAAATGATGTCT
GTTGTCCAGACTAAACCAGTGCAAACCTCTGCAATTACAGGGCAGGCATCCACTGGACCA
TTGACACAGATCATACAGACCAAAGGACCGCTACCCGCAGGAACAATACTGAAGCTTGTA
ACATCTGCTGATGGGAAACCCGCAACTATAATTACCACCACGCAAGCTGGAGGCACAGGC
ACCAAGCCTACCATCCTGGGTATTAGCAGTGTTTCTCCAAATACCACTAAGCCTGGGACT
ACCACCATTATTAAGACAATTCCCATGTCTGCCATTATCACCCAAGCAGGTGCCACAGGT
GTAACAAGCACTGCTGGTATTAAGTCACCAATTACCATTCTTACAACCAAAATGGGGACC
TCTGGCACTGGAACCCCAGCCAAAATAATTACAGCTGTTCCGAAACATACAGCTACTCTT
GGACAACAAGGATTAACACAGGTGGTCCTAAAAGGAGCTCCTGGGCAGCCTGGCACAATT
CTTCGTACTGTCCCAATGAGTGGCATGAGACTTGTCACTCCAGTTTCTGCAGTGAAACCT
ACTGTCACTACCCTTGTTGTGAAAGGCACCACAGGTGTCACCACTCTTGGCACTGTTTCA
GGGACTGTTTCAACAAGTTTAGCCGGAGGTGGGGTTCATAACAGTGCTTCTCTCGCAACA
CCAATAAACACTCTGGGCACAATTGCTACGCTCTCCAGTCAGGTGATCAACCCTGGTGCC
ATTACTATGTCTGCAGCTCAGACTTCCCTCACTGCAGCATCTGGACTGTCCACTCCCACC
ATTACCATGCAGCCGCTTTCTCAGCCTACACAAGTGACATTAATTACAACTCCTAGTGGT
GTTGAGGCCCAGCCTGTTCACGATCTTCCAGTGTCCATACTGGCTTCACCAACTTCTGAC
CAGCCCACAGCTACTGTAACTATTGCAGATTCTGGCCAAGGAGACCAACCTGGCACTGTC
ACTTTGGTTTGCTCCAACCCACCTTGTGAAACTCATGAGACTGGAACCACCAATACTGCC
ACCACCAGTCTTGTAAATCTCAGCAGCATTGTCCAGCAACCTGGTGGCACTTTACAATTT
GTTTGTGAAGGTCAAGAGCCTGGAACATATGTAGCAACTTCTTTAGCACAAGCAAATGGC
AGTGTGGTGAGAGTTTGCTCAAATCCTCCATGTGAAACCCATGAAACAGGAACTACCCAG
ACATCAACTACAGCCATGGCCAGTATTGGCCAAACAGGAATAGTCACAAGGGTTTGCTCC
AACCCACCTTGTGAAACCCATGAAACTGGAACCACTCAAACTCCAACAACAGCTATGTCA
AATATCGGCAGTGGGGAGATGGGAACTGTGACCAGAATTTGTTCAAATCCCCCTTGTGAA
ACCCATGAAACAGGAACTACTCAAACTTCTACCACAGCGATGGCAAATATGGACAGTGGG
CAGGGTGAAAGCAGTAAGAAAATTTGCTCTAATCCACCGTGTGAGACTCATGAAACAGGC
ACAACTCCAACAGCTACAACAGCTAGATCAAATATTGGCAAAGAACAAACAGACAATACT
CAAAGGCTGTCTAAACCGCCATGTGAGACGATGCAAACTGTATCTACCCATACAGTCATG
ACTGTGCCTGATAGCTTTACCAGTGGACTGACTAGTTCCAACCTTCCTTGTGAAACACAT
GAGACGAATATCACCAGCACAACCACAGATGTAACTGCTAACGTCGGAACAGACCAGTCA
CAAAATGTTGTTAATGTATGCTCTGACCCACGTTCAGAGACCTATGAAACTGGCACCATT
AATACTCCTACCACAGCAAGATCTAACATTGGTTCTGGGCAAACGGAGAATGCCAATGAA
ACGCCCTGTGAGACCCATCAGGTGACTTCCACACAAACCAGGATGACCATATCGGAGTCA
AATAAAGTACCATCTTCAAGTAGTGAGGCCAACGATATTGTTGAAGACAACCAAACTGGG
AGCTCTGTGTGTTCCAATCCATCCTGTGAGACTCATGAAACTAACACCACCAATACCTCA
ACTACTTCTAGGTCCAACATTGGGGCAGAGCAGGCTGCAAATATCCAGCAGCTTTCCAAG
AACACAGACCAGGTGATCTCTGCAGGAAGTACAAAGACTGAACCAAACATCCTAGAGCAG
CGGGTGTGCTCCAACCCTCCTTGTGAAACCCATGAAACCAATACTACCAACACTGCAACA
ACATCAACATCTAATATAGACAACCAGCCTACTGCTCCTCCTACTCAGCAAGTCTGCTCT
AACCCTCCTTGTGAAACTCACGAGACTGGAACAACCCATACGGCCACCACAGTTACAGCC
AGCATGGGAACCAACGAAGATCAGCCACCTGCCAGTGGGGAAGAAGAAGAACAACAGTCT
GAGCAGGCTGATGAATCTATCCAAAGCAGCAACATCAGTACTGGGTCCTCATCCTCCCCT
GTTACTATAGTGGCTGTAGCTGCTACCGCATCCTCCTCTCCAGTACACTCTACAGTGCCA
GCTGTCTCCAGAGCAGTAACAACTGTAACACAGTCCAGGCAACTTCCTGGACCGTCTGTG
CCGAACATCTCTTCTATTACTGAACCATCCTCTGACGTTGCTGTGCAAGGATCTGAAGCA
GTTTCGTGCCCGGAGCAGTCAAAAGCTCCACTTTCCTCCGATATTCTTCAGCCATCTAAG
GACGTACAATCTGAGGCAGAAGCACTTCAGCAGCCAATGGATAGTGCAGTGCCAGAACAC
AATGCAGCAGAGCTGCATGCAGCTATTGAAGCAGAATCCAGTCAGAATGCTGCAGTGGCT
GCTGAACAACATGCAGTAGAACAGCTGTCTCTTCCTCAAGAACTTATGGCAGAAGGGCAG
ACAACCACGCTGATGGTGACAGGACTGACACCCGAAGAACTTGCTGTAACTGCTGCAGCA
GAGGCAGCAGCTCAAGCCGCAGCAACAGAGGAAGCCCAGGCATTGGCCATCCAAGCTGTG
CTACAGGCAGCCCAGCAGGCAGTGATGGGTTTCCAAGCTGCATCTGGGGAACCTATGGAT
ACCTCTGACGTGGCTCATGCTGAGCTGCGTCACCTGACGACTGAAGGGCCTACAACAACC
ATTCCCATTGTCCTTACACAGCAAGAGCTAGCAGCCTTGGTGCAGCAGCAACAACTCCAG
GAGCAACAGCAGATTCAACAACATCAGCTCCAACAGCAGCAGCTTCAGCAACTGCAACAG
CAGCAGTTGCAACAACAGCAACAGCTTCACCAGCAGCAGCTGCAACATTTACAGCAATTG
CACCACGTGCCTACAGAGGCCCTCGCACCAGCTGATAGTCTTAATGATCCTGCCAGTGAA
AGCAATGGGCTCAGTGAACTAACTAGTGCTGTGACCAACGCAGTGGCACTTTTGCCAACT
ACAGCCACTGACAATTTGACTGTATCCAATACTTTCACAGCTCCTCAACCTGTTGTGATT
GCCAGTCCTGCCAAACTGCAGGCTGCAGCTGCTCTAACAGAGGTTGCGAACGGAATTGAA
TCGAAACAGGATTCCCCACCACAACAGGCTAAGGCTCCAGTGAAAAAAGAGAATCAGTGG
TTTGATGTGGGAGTAATAAAAGCCACTAATATGATGGTGACACATTATTATCTGCCTGCT
GATGACTCTGTTAATGATGATGACTCTGTTGTTCTGCCGGATTACAGCCAGTTGAAGAAG
CAAGAGCTGCAGCCTGGCACAGCTTACAAATTCCGGGTTGCTGGTGTAAATGCTTGTGGT
CGTGGACCCTACAGTGAAATATCAGCTTTTAAAACTTGTTTGCCTGGTTTCCCTGGCGCA
CCATGTGCCATAAAGATCAGCAAGAGCCCTGATGGAGCTCACCTTACCTGGGAGCCTCCC
TCTGTGACCTCAGGCAAAATCATAGAGTACTCCGTTTATCTGGCCATCCAGAGCAGCCAA
GCCAGTGGAGAACAGAAGTCAACACCAGCTCAGCTGGCTTTCATGAGGGTGTACTGTGGC
CCTAACCCTTCTTGTTTGGTGCAGTCCTCCAGTCTTTCCAATGCTCATATAGATTACACC
ACAAAACCTGCCATAATCTTTCGCATTGCTGCTCGAAATGAAAAGGGCTATGGACCTGCC
ACCCAAGTCAGGTGGCTGCAGGAATCTAACAAGGATGGATCAGCTGGGAAGCCTGCCAAT
AAGCGGCCCGTATCCTCCCCTGATATGAAAACGGCTCCAAAGAAAGCAAAGTCTGATCAC
GCCTGA
Protein sequence predicted based on nucleotide sequence
MAATPALSTAGALQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVY NTSTNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKR LKAKAPKNGPPPCPRLGHSFSLVGSKCYLFGGLANDSEDPKNNIPRYLNDLYILELRAGS GVVAWDVPITYGILPPPRESHTAVVYTDKDNKKSRLVIYGGMSGCRLGDLWILDIDTLTW SKPSLNGVAPLPRSLHSATTILNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLE SMSWEHIVIDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETE KPPAPARVQLVRANTNSLEVSWGAVPTADSYLLQLQKYDIPAAAAAMSPANPVPSVPVNP PKSPAPASAAPTVQQVAHTGITLLPQAASLPPTTATIQVLPTMPGSPIAVSAALRTQAVP AVLKVGGQQSTLSTPLVTVRPANQISKAPVTVTSLPPGVRMVVPAQSTQGAPIGSSQPMS GMAALAAAAAATQKIPPSSAPTMLGVPAGATMVKSVAVPSGVTSLPTTVKVASSPIMVSN PATRMLKTAAAQVGTSVPGSNTQNRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVK SPISVPGGSALISNLGKMMSVVQTKPVQTSAITGQASTGPLTQIIQTKGPLPAGTILKLV TSADGKPATIITTTQAGGTGTKPTILGISSVSPNTTKPGTTTIIKTIPMSAIITQAGATG VTSTAGIKSPITILTTKMGTSGTGTPAKIITAVPKHTATLGQQGLTQVVLKGAPGQPGTI LRTVPMSGMRLVTPVSAVKPTVTTLVVKGTTGVTTLGTVSGTVSTSLAGGGVHNSASLAT PINTLGTIATLSSQVINPGAITMSAAQTSLTAASGLSTPTITMQPLSQPTQVTLITTPSG VEAQPVHDLPVSILASPTSDQPTATVTIADSGQGDQPGTVTLVCSNPPCETHETGTTNTA TTSLVNLSSIVQQPGGTLQFVCEGQEPGTYVATSLAQANGSVVRVCSNPPCETHETGTTQ TSTTAMASIGQTGIVTRVCSNPPCETHETGTTQTPTTAMSNIGSGEMGTVTRICSNPPCE THETGTTQTSTTAMANMDSGQGESSKKICSNPPCETHETGTTPTATTARSNIGKEQTDNT QRLSKPPCETMQTVSTHTVMTVPDSFTSGLTSSNLPCETHETNITSTTTDVTANVGTDQS QNVVNVCSDPRSETYETGTINTPTTARSNIGSGQTENANETPCETHQVTSTQTRMTISES NKVPSSSSEANDIVEDNQTGSSVCSNPSCETHETNTTNTSTTSRSNIGAEQAANIQQLSK NTDQVISAGSTKTEPNILEQRVCSNPPCETHETNTTNTATTSTSNIDNQPTAPPTQQVCS NPPCETHETGTTHTATTVTASMGTNEDQPPASGEEEEQQSEQADESIQSSNISTGSSSSP VTIVAVAATASSSPVHSTVPAVSRAVTTVTQSRQLPGPSVPNISSITEPSSDVAVQGSEA VSCPEQSKAPLSSDILQPSKDVQSEAEALQQPMDSAVPEHNAAELHAAIEAESSQNAAVA AEQHAVEQLSLPQELMAEGQTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAV LQAAQQAVMGFQAASGEPMDTSDVAHAELRHLTTEGPTTTIPIVLTQQELAALVQQQQLQ EQQQIQQHQLQQQQLQQLQQQQLQQQQQLHQQQLQHLQQLHHVPTEALAPADSLNDPASE SNGLSELTSAVTNAVALLPTTATDNLTVSNTFTAPQPVVIASPAKLQAAAALTEVANGIE SKQDSPPQQAKAPVKKENQWFDVGVIKATNMMVTHYYLPADDSVNDDDSVVLPDYSQLKK QELQPGTAYKFRVAGVNACGRGPYSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPP SVTSGKIIEYSVYLAIQSSQASGEQKSTPAQLAFMRVYCGPNPSCLVQSSSLSNAHIDYT TKPAIIFRIAARNEKGYGPATQVRWLQESNKDGSAGKPANKRPVSSPDMKTAPKKAKSDH A
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
