fgfr4-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAGTCCCAAGGTGAGTGTGAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCAGAACTTTGCCAGGT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711533
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2421nts
Open reading frame: 1 to 2421

ATGCAGAACTTTGCCAGGTTGCTGTTGGGAGTTCTGTTGGTTGCTACTTTAAGCTCGTGC
AGACCAGCGTTATCCGAAGATGAAGCCAACTGGAAGGAACCTGAGGTTGAAGAACATCTA
CTTCTAGACCCCGGAAATGCTCTGCGACTGTTTTGTGACACCAACCAAAGCAACAGCATC
AACTGGTACCGCGAACAGGATCGTTTATTGCCAGGAGGGAAGATTCGCATGGTGGGGACT
GTGCTAGAAGTCTCAGATGTGACATATGAAGACTCAGGACTCTACATATGCGTGGTCAGA
GGAACAGGCAAAATCCTTAGAAGGTTCTCCATATCCGTTGTTGACTCGTTGGCATCTGGA
GATGAAGAAGATGATGAGGATGGCCGTAGGGAGGACACAACCGCTGATATTAATGAGGAG
CCAGTTTATTTTTTCCAAGCACCATACTGGACACAGCCTCACCGCATGGACAAGAAACTT
CATGCTGTGCCAGCTGGTAACACAGTCAAGTTCCGCTGTCCAGCTGGTGGGAGTCCCCTT
CCCACTATTCGATGGCTTAAAAATGGCAGGGAATTTCGAGGAGAACACAGAATTGGTGGG
ATCCAGCTTCGACACCAACACTGGAGTCTGGTAATGGAGAGTGTGGTTCCATCAGACCGT
GGCAACTACACCTGTGTGGTAGAGAACAGAGTTGGCAGCCTCACATATACCTACTTTCTG
GATGTGCTTGAGAGGTCTTCTCACCGTCCTATCCTACAGGCTGGCCTTCCAGCAAACACA
ACAGCACGCGTGGGTAGCGATGTTGAATTCTACTGCAAAGTATACAGCGATGCTCAGCCA
CATATCCAATGGCTTAAACACATTGAAGTGAATGGAAGCCGATTTGGTCCTGATGATTTT
CCATATGTACAGGTGCTGAAGACTGCAGATATTAACACTTCGGAGGTAGAGGTGCTTCAC
CTACGGAATATCACTATGGAGGATGCAGGGGAATACACATGTCTGGCGGGCAATTCTATT
GGTCTTTCTCATCAGTCTGCTTGGCTGACTGTGCTTTCAAACGAAGATTTCCTTGAGCAA
GCTGAGCCAGCAGAGTCAAGATATATGGACATAATAATATACACTTCTGGATTCCTGGCT
GTAGCCATGGCCATCGTCATAGTGGTTCTGTGCCGCATGCAGACACCGCACAGCAAGCAG
ACTCTGCAACCACCAGCTGTTCATAAACTGGCCAAGTTCCCCCTCATACGGCAGTTCTCT
TTGGAGTCCAGCTCATCTGGGAAGTCCAGTGCTCCACTGATTCGCATAACTCGTCTCTCC
TCAAGCTGTGCCCCCATGCTGCCTGGTGTCATGGAGGTTGAGTTACCACTGGACGCTAAA
TGGGAGTTCCCTAGAGACAGGCTTGTTCTGGGAAAGCCACTTGGAGAGGGCTGCTTTGGC
CAAGTGGTAAGAGCAGAGGGATATGGAATTGAGAAAGACCGGCCTGAGAAACCAGTTACC
GTTGCAGTTAAGATGCTCAAAGATAATGGCACTGACAAGGACTTATCAGATCTGATCTCT
GAAATGGAGTTGATGAAGGTCATTGGAAAACACAAAAATATAATAAACTTGCTCGGCGTC
AGCACTCAAGAGGGGCCATTATTTGTTATAGTTGAATATGCTTCTAAGGGGAATCTGCGT
GAGTTTCTACGTGCCAGGCGCCCTCCCACACCGGAAGATGCCTTTGATATCACCAAGGTT
CCAGATGAACTTTTGTCCTTTAAGGACCTAGTGTCTTGTGCTTATCAGGTTGCCCGTGGC
ATGGAGTACCTTGAATCTAAGAGGTGTATACACCGGGATCTGGCAGCCAGAAATGTTCTT
GTGGCAGAAGATAATGTCATGAAGATTGCAGATTTTGGCTTGGCACGAGGGGTTCACGAT
ATTGACTATTACAAGAAAACTAGTAATGGTCGACTGCCTGTTAAATGGATGGCTCCAGAA
GCTTTATTTGACAGAGTTTACACCCACCAAAGTGACATTTGGTCATTTGGAGTGTTGACA
TGGGAGATCTTTACTCTTGGAGGGTCTCCATATCCTGGAATTCCAGTAGAAGAACTATTT
AAACTCTTACGGGAAGGACATCGAATGGACAAACCATCCAACTGTACTCATGAATTGTAC
ATGTTGATGAGGGAGTGCTGGCATGCAGTTCCATCTCAGAGACCAACATTTAAACAGCTG
GTTGAACAACTGGATAGGATCCTCACAGCTGTTTCTGAAGAGTATCTGGACTTATCTATG
CCATTTGAACAGTATTCTCCCTCCTGTGAAGATTCTTCAAGCACATGCTCTTCATCCGAT
GACTCTGTTTTTGCCCACGATCCAGTGCCATCCTCTCCCTGTGTCTTTAATTATCACAAT
GTTCACACTCACCTTGGGACTTGA

Protein sequence predicted based on nucleotide sequence

MQNFARLLLGVLLVATLSSCRPALSEDEANWKEPEVEEHLLLDPGNALRLFCDTNQSNSI
NWYREQDRLLPGGKIRMVGTVLEVSDVTYEDSGLYICVVRGTGKILRRFSISVVDSLASG
DEEDDEDGRREDTTADINEEPVYFFQAPYWTQPHRMDKKLHAVPAGNTVKFRCPAGGSPL
PTIRWLKNGREFRGEHRIGGIQLRHQHWSLVMESVVPSDRGNYTCVVENRVGSLTYTYFL
DVLERSSHRPILQAGLPANTTARVGSDVEFYCKVYSDAQPHIQWLKHIEVNGSRFGPDDF
PYVQVLKTADINTSEVEVLHLRNITMEDAGEYTCLAGNSIGLSHQSAWLTVLSNEDFLEQ
AEPAESRYMDIIIYTSGFLAVAMAIVIVVLCRMQTPHSKQTLQPPAVHKLAKFPLIRQFS
LESSSSGKSSAPLIRITRLSSSCAPMLPGVMEVELPLDAKWEFPRDRLVLGKPLGEGCFG
QVVRAEGYGIEKDRPEKPVTVAVKMLKDNGTDKDLSDLISEMELMKVIGKHKNIINLLGV
STQEGPLFVIVEYASKGNLREFLRARRPPTPEDAFDITKVPDELLSFKDLVSCAYQVARG
MEYLESKRCIHRDLAARNVLVAEDNVMKIADFGLARGVHDIDYYKKTSNGRLPVKWMAPE
ALFDRVYTHQSDIWSFGVLTWEIFTLGGSPYPGIPVEELFKLLREGHRMDKPSNCTHELY
MLMRECWHAVPSQRPTFKQLVEQLDRILTAVSEEYLDLSMPFEQYSPSCEDSSSTCSSSD
DSVFAHDPVPSSPCVFNYHNVHTHLGT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable