pepd (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACAGCTGCTGGCTGAGCAC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGTTCTGCTGCAGGT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711640
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1494nts
Open reading frame: 1 to 1494

ATGAGTTCTGCTGCAGGTCCATCATTCTGGTTGGGGAACGAAACCCTGAGAGTTCCAGCC
GCTCTGTTTGCTCTGAACAGGAAACGTCTATGTGACATACTTAGGCAGAATAAAGATTTG
CCAAAGGGATCGATTGTACTTCTGCAAGGAGGAGAAGCGACACAGAGATACTGCACAGAT
ACTGGCACCCTGTTCCGCCAGGAGTCCTTTTTTCACTGGACTTTTGGAGTGACTGAACCT
GGCTGCTATGGAGCTGTTGATGTTGATACAGGAAAATCAATTGCCTTCATTCCAAAGCTT
CCTGAAAGTTATGCAGTATGGATGGGAAAAATACACCCACCAGAACACTTCAAGGAGAAG
TATGCCATAGATGAGATTTATTTCACCTGCGATATATCCAGTGTTCTGAAAGCAAAGACC
CCATCTGTTTTGCTTACTTTGCGTGGTGTTAATACTGACAGTGGAAGTGTTTGCAAGGAA
GCTTCCTTTGAAGGAATTAGTGAATTCAGTGTAAATAACACCCTACTGCATCCAGAAATT
GTTGAATGCCGTGTGTTTAAGACAGACATGGAGTTGGAGGTTCTTCAATACACAAATCGC
ATCTCCAGTGAAGCCCACAAGGAGGTCATGAAGGCAGCAAGAGTTGGAATGAAAGAATAC
GAACTGGAGAGTGTTTTTCTTCACTATTGCTATGCACGTGGAGGCATGAGACATACTTCT
TATACCTGTATATGTGGCAGTGGTGACAATTCTTCAGTTTTGCATTATGGCCATGCGGGA
GCTCCTAATGATAAGACTGTGATGGATGGTGACATGTGCTTGTTTGATATGGGAGGGGAA
TATTACTGTTACAGTTCTGACATTACCTGCTCCTTTCCGGCCAATGGCAAGTTCACTCCA
GATCAGAGGGCTGTTTATGAGGCTGTGCTGAAGTCATCACGTGCGGTCATGAAAGCTGTA
AAACCAGGTGTTGCTTGGCCGGATATGCATCGCCTTGCTGACAGAGTCCATCTGGAGGAG
CTGACCAAAATTGGCATTCTGAAAGGCGATGTTGACGACATGATAAAGACCCACATGGGT
GCAGTTTTCATGCCACACGGTCTTGGACATTTTCTAGGAATTGATGTTCATGATGTCGGA
GGTTATCCAGAGGGTATTGACCGCATTGACCTGCCTGGTCTAAAAAGCCTTCGCACAGCC
CGTACCCTCCAAGAAAGAATGGTACTCACCATAGAACCAGGAATTTATTTTATTGACCAT
GTACTAGACCAAGCACTGGCCAACCCTGCTCAGTCCTGCTTCATTAACAATGCCGTCCTT
CAACGCTTTCGAGGATGTGGTGGGGTGCGCATTGAAGACGATATTGCAGTGACAGCCAAT
GGAATGGAGCTGCTCACATGTGTCCCACGCACAGTGGAGGAAATCGAATCTTTCATGGCA
GAAGCTAAGGGCAATAACAAAACCTTCGAGAAGGTGGTGCTCAGCCAGCAGCTGTGA

Protein sequence predicted based on nucleotide sequence

MSSAAGPSFWLGNETLRVPAALFALNRKRLCDILRQNKDLPKGSIVLLQGGEATQRYCTD
TGTLFRQESFFHWTFGVTEPGCYGAVDVDTGKSIAFIPKLPESYAVWMGKIHPPEHFKEK
YAIDEIYFTCDISSVLKAKTPSVLLTLRGVNTDSGSVCKEASFEGISEFSVNNTLLHPEI
VECRVFKTDMELEVLQYTNRISSEAHKEVMKAARVGMKEYELESVFLHYCYARGGMRHTS
YTCICGSGDNSSVLHYGHAGAPNDKTVMDGDMCLFDMGGEYYCYSSDITCSFPANGKFTP
DQRAVYEAVLKSSRAVMKAVKPGVAWPDMHRLADRVHLEELTKIGILKGDVDDMIKTHMG
AVFMPHGLGHFLGIDVHDVGGYPEGIDRIDLPGLKSLRTARTLQERMVLTIEPGIYFIDH
VLDQALANPAQSCFINNAVLQRFRGCGGVRIEDDIAVTANGMELLTCVPRTVEEIESFMA
EAKGNNKTFEKVVLSQQL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable