stat6 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACTCCATCCAGTTGGTATCTGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTCAAGTGTCTCTTTGGAGT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711873
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2373nts
Open reading frame: 1 to 2373

ATGGCTCAAGTGTCTCTTTGGAGTTATGTGTCCAGAATTGGTCCTGAGAAGTTTGAAAAT
ATGTACCAAGAATTTCCAATAGAAGTTCGGAGTCTCATGGCTGATTTCTTGGAGAATCAA
CCCTGGGAGTATGTTACTGGATCAGATTCATTCTGCTTGGAATTGGCAAATACTTTGATA
GAGCGTTTATTGGAAGAACTTAAGAAGATCTCCAGAAATAGTGGAGTTGCAGTTCATCTG
ATTCATCAGTATGTACAGAGGATATCAAAGCAGGATTCAGTGCAGACAGTGCTGCAGATT
AAATGTCTTCTGGAAAATGAAAAGGCGGTGGTCTTGAAACAGGTTCCACGCTTAAGCGTG
AGCCTCAACCAGAGAAAGGAGGAAATGCAGTTCAATGTTCAGATAATGAAGCTAAAGCAT
AAGTTACAGCAAGTTACAATGGTTCAACAACAGTTAAGAGATGCAAAGAAGAACATGGGC
TTTCACAATCAAATGCACAACGTGGCAAGTCTACATATGCAATGGGAAGTGCTCGTAAAG
GAATACTTTCAAATTTTGGAGAGTGCTCAAACTCAGGCCCTTAAAAGGATCAGTATCTGG
AAAAGACAGCAACAGCTTGCAGGGAATGGTGCCCCATTTGATGAGAATCTGATTCCCCTG
CAGGAAAGAATGGAAACAATATTTGCAATGTTTCAAGATATTGATCGAGCATTGAAGGAC
TTTAGTTTATTGGGGGAGGAGGTGCCATCAGGGCTTCCTGAAAACATGAACAGTTCCCTT
CGGACTCTCATTACAAGTTCTCTCTTGGTTGACAAACAGCCCCCACAAGTGCTGAAGACA
CAGTCAAAGTTCCAAGCCAGCGTGAACTTCTTGCTTGGATCTCGACTACTCAATAGTTCT
CCCAAAATGCCTGTAGTGAGGGCAACTATCATAACTGAAAAAAAAGCACAGGAGCTCTAT
CAGACACCTATCGGGGAGTCCTGGAATGAGGGCGCTGGTGATATTGAGAATGGAAAATCT
GTCCTGGAGATTGCGCCGAACTCCAAAACGTGTGGTGCTGTTTTCAAAAATTTGTTGCTG
AAGAAGATAAAACGGTGTGAGAGAAAGGGTTCCGAATCAGTAACGGAAGAGAAATGCGCT
GTGCTGTTTATAGCTGAATTCAACCTGAACTCCATAAACACAACGTTCCACATACAAGCC
TTATCCCTACCTGTCGTGGTTATTGTTCACGGGAACCAGGACAACAATGCTAAAGCCACC
ATTCTGTGGGACAATGCTTTCTCAGAGGTGGAACGTCGCCCCTTCTTTGTAGAGGAAAAG
GTGCCCTGGTCCAAGATGTGCCACACCCTTAATATGAAGTTTGTTTCTGAAGTGGGGACA
AAGCAAGAGCTGCAAAGAGAGCATTTTATGTTTCTTGCCCAAAAGATTTTCAATGACAAC
AGCTTAAATGATGACCAAGACCGGATGGTTTCATGGTCTCAGTTCAATAAGGAGCCTCTT
CGGGAAAGGAACTTTACATTTTGGCAGTGGTTTGATGGTGTTGTGGATCTCACCAAAAAG
TGTTTGAAAGATTATTGGTCTGACAGGTTGATTATAGGCTTTATCAGCAAGCAGTATGTT
CACACGCTACTAAGTACTCAGCCTGATGGAACCTTCCTCCTGCGTTTCAGCGACTCGGAG
ATTGGTGGCATCACCATAGCACATATTTTAAAGAGAGAGGACAACTCAGCACAGATCCAG
AATATCCAACCATTCACTGCTAAAGATCTGAACATTCTTTCTCTTGGTGACCGCGTTAGA
GATCTGAAACAGCTAAAATATTTGTACAGTAACAAATGCAAAGATGACGTCTTTGGAAGA
CACTACTCAAAAAAAACTTCCAAAACGCCTGATGGATACACCCCAACATTGATAACATTA
AAGGTTGCTGGCGGGGATACCACCAACCCAGTTAACCCCCTGATCAGTAACGAACCAACA
TGGCCGGTTAATGGAGTGGGAGCAATTGGTAGTATCTCACCGCCGCCGCCACCACCACCA
AGACCCTTACAGCCACCCATCTTTCATCACGTTGAAGGCCATCCTACTCCACCCTACTCC
ACTCCAGACTTTGTACCCATGTTGCCTTATCCTGAGGGGTGTGATCCAGGATTGCAGTGG
AATGGACCGATCTTCATTCCTCCAGTGCAAATCATGCCGCCACAACCCTTTCACTCGCAA
GAACCATTTCCCAACATGGTCCTAAACCCAAATGATATCAACATGATGGACGAACTCCAA
ACCATCGAGGACGCCTTAGGAGAGGATTTCAGTTCATCCCATGTGTTGGCCCCACTGAGC
ATAGTCCACGATACAGATACCAACTGGATGGAGTGA

Protein sequence predicted based on nucleotide sequence

MAQVSLWSYVSRIGPEKFENMYQEFPIEVRSLMADFLENQPWEYVTGSDSFCLELANTLI
ERLLEELKKISRNSGVAVHLIHQYVQRISKQDSVQTVLQIKCLLENEKAVVLKQVPRLSV
SLNQRKEEMQFNVQIMKLKHKLQQVTMVQQQLRDAKKNMGFHNQMHNVASLHMQWEVLVK
EYFQILESAQTQALKRISIWKRQQQLAGNGAPFDENLIPLQERMETIFAMFQDIDRALKD
FSLLGEEVPSGLPENMNSSLRTLITSSLLVDKQPPQVLKTQSKFQASVNFLLGSRLLNSS
PKMPVVRATIITEKKAQELYQTPIGESWNEGAGDIENGKSVLEIAPNSKTCGAVFKNLLL
KKIKRCERKGSESVTEEKCAVLFIAEFNLNSINTTFHIQALSLPVVVIVHGNQDNNAKAT
ILWDNAFSEVERRPFFVEEKVPWSKMCHTLNMKFVSEVGTKQELQREHFMFLAQKIFNDN
SLNDDQDRMVSWSQFNKEPLRERNFTFWQWFDGVVDLTKKCLKDYWSDRLIIGFISKQYV
HTLLSTQPDGTFLLRFSDSEIGGITIAHILKREDNSAQIQNIQPFTAKDLNILSLGDRVR
DLKQLKYLYSNKCKDDVFGRHYSKKTSKTPDGYTPTLITLKVAGGDTTNPVNPLISNEPT
WPVNGVGAIGSISPPPPPPPRPLQPPIFHHVEGHPTPPYSTPDFVPMLPYPEGCDPGLQW
NGPIFIPPVQIMPPQPFHSQEPFPNMVLNPNDINMMDELQTIEDALGEDFSSSHVLAPLS
IVHDTDTNWME

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable