actc1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  actc1
Gene Name:  actin, alpha, cardiac muscle 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAAGCACTTCCTGTGCACAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTGTGACGACGAGGAGA, XGC Collection: IRBG
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712117
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1131nts
Open reading frame: 1 to 1131

ATGTGTGACGACGAGGAGACTACCGCCTTGGTGTGCGACAATGGCTCCGGGCTGGTGAAG
GCTGGGTTCGCTGGGGATGATGCCCCCCGTGCTGTGTTCCCCTCCATCGTTGGTCGCCCC
CGTCATCAGGGTGTCATGGTGGGTATGGGTCAGAAAGACTCCTACGTAGGTGATGAAGCT
CAGAGCAAGAGAGGTATCCTGACCCTGAAGTACCCCATTGAGCACGGTATCATCACTAAC
TGGGATGATATGGAGAAGATCTGGCACCACACCTTCTACAATGAGCTGCGTGTGGCCCCT
GAAGAGCACCCCACCCTGCTCACTGAGGCCCCACTGAACCCCAAGGCCAACCGTGAAAAG
ATGACACAGATCATGTTTGAGACCTTCAACGTCCCAGCCATGTATGTTGCCATCCAGGCT
GTGCTGTCCCTGTACGCTTCTGGTCGTACCACAGGTATCGTTCTTGACTCTGGTGATGGT
GTCACCCACAATGTCCCCATCTATGAAGGTTATGCTCTGCCCCATGCCATCCAGCGTCTG
GACCTGGCTGGTAGAGACCTCACAGATTACCTCATGAAGATCCTGACTGAACGTGGCTAC
TCCTTTGTGACAACAGCTGAAAGGGAAATTGTCCGTGACATCAAGGAAAAGCTGTGCTAT
GTGGCTTTGGACTTTGAGAATGAAATGGCCACCGCTGCCTCATCCTCCTCCCTGGAGAAG
AGCTATGAGCTTCCTGACGGTCAGGTCATCACCATTGGAAATGAACGTTTCCGTTGCCCT
GAGACCCTCTTCCAGCCTTCCTTCATTGGTATGGAATCTGCCGGTATCCATGAAACTACC
TACAACAGCATCATGAAATGCGACATTGATATCCGCAAGGACCTGTACGCCAACAACGTC
CTCTCTGGTGGTACCACCATGTACCCTGGTATTGCTGACAGAATGCAGAAGGAGATCACT
GCCCTGGCCCCCAGCACCATGAAGATCAAGATCATTGCCCCACCTGAGCGTAAATACTCC
GTCTGGATCGGTGGCTCCATCTTGGCCTCTCTGTCCACCTTCCAACAGATGTGGATCAGC
AAACAGGAATACGATGAGGCAGGACCCTCCATTGTGCACAGGAAGTGCTTCTAA

Protein sequence predicted based on nucleotide sequence

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIQRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
LSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS
KQEYDEAGPSIVHRKCF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable