| Gene: |
Gene Symbol:
actc1 Gene Name: actin, alpha, cardiac muscle 1 Sequence |
|---|---|
| Original Clone ID: | None |
| Keyword: | None |
| Species: | Xenopus laevis |
| Type: | cDNA |
| Vector Name: | pDONR223 Format: FUSION |
| Source: | Xenopus Gene Collection |
| Description: | None |
| Comments: | The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAAGCACTTCCTGTGCACAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTGTGACGACGAGGAGA, XGC Collection: IRBG |
| Mutation/ Discrepancy : |
No / No |
| Publications: | None |
| Authors: |
Center for Cancer Systems Biology Cincinnati Children's Hospital Medical Center MRC National Institute for Medical Research University of Virginia School of Medicine Aaron Zorn David E. Hill Michael J. Gilchrist Todd Stukenberg Xenopus Gene Collection |
|
XlCD00712117 Price: Login for Pricing AVAILABLE No restriction Special MTA: None |
|
Insert sequence: 1131nts
Open reading frame : 1 to 1131 ATGTGTGACGACGAGGAGACTACCGCCTTGGTGTGCGACAATGGCTCCGGGCTGGTGAAG
GCTGGGTTCGCTGGGGATGATGCCCCCCGTGCTGTGTTCCCCTCCATCGTTGGTCGCCCC
CGTCATCAGGGTGTCATGGTGGGTATGGGTCAGAAAGACTCCTACGTAGGTGATGAAGCT
CAGAGCAAGAGAGGTATCCTGACCCTGAAGTACCCCATTGAGCACGGTATCATCACTAAC
TGGGATGATATGGAGAAGATCTGGCACCACACCTTCTACAATGAGCTGCGTGTGGCCCCT
GAAGAGCACCCCACCCTGCTCACTGAGGCCCCACTGAACCCCAAGGCCAACCGTGAAAAG
ATGACACAGATCATGTTTGAGACCTTCAACGTCCCAGCCATGTATGTTGCCATCCAGGCT
GTGCTGTCCCTGTACGCTTCTGGTCGTACCACAGGTATCGTTCTTGACTCTGGTGATGGT
GTCACCCACAATGTCCCCATCTATGAAGGTTATGCTCTGCCCCATGCCATCCAGCGTCTG
GACCTGGCTGGTAGAGACCTCACAGATTACCTCATGAAGATCCTGACTGAACGTGGCTAC
TCCTTTGTGACAACAGCTGAAAGGGAAATTGTCCGTGACATCAAGGAAAAGCTGTGCTAT
GTGGCTTTGGACTTTGAGAATGAAATGGCCACCGCTGCCTCATCCTCCTCCCTGGAGAAG
AGCTATGAGCTTCCTGACGGTCAGGTCATCACCATTGGAAATGAACGTTTCCGTTGCCCT
GAGACCCTCTTCCAGCCTTCCTTCATTGGTATGGAATCTGCCGGTATCCATGAAACTACC
TACAACAGCATCATGAAATGCGACATTGATATCCGCAAGGACCTGTACGCCAACAACGTC
CTCTCTGGTGGTACCACCATGTACCCTGGTATTGCTGACAGAATGCAGAAGGAGATCACT
GCCCTGGCCCCCAGCACCATGAAGATCAAGATCATTGCCCCACCTGAGCGTAAATACTCC
GTCTGGATCGGTGGCTCCATCTTGGCCTCTCTGTCCACCTTCCAACAGATGTGGATCAGC
AAACAGGAATACGATGAGGCAGGACCCTCCATTGTGCACAGGAAGTGCTTCTAA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: End read sequencing |
|
Mutations: None |
Discrepancy: None |
| 5' Linker Sequence: None | |
| 3' Linker Sequence: None | |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
spectinomycin Bacterial Selection Condition: 100 ug/mL spectinomycin Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin. Comments: None |
| Protein Expression Results: | None |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pDONR223 |
pDONR223 in advanced viewed |
| Synonyms: | '' | |
| Sequencing Primer: | Forward:
M13F
Reverse: M13R |
|
| Description: | Recombinational donor/master vector; spectinomycin resistance; recombinational cloning. | |
| Comments: | pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors. | |
| Size (bp): | 5005 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | Gateway, donor (entry), recombinational cloning | |
| Author Name: |
Invitrogen |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | pBR322 origin | pBR322 bacterial origin | 4339 | 4958 |
| death cassette | ccdB | ccdB negative selection gene (death cassette), LOST in recombined (with insert) form | 1200 | 1409 |
| primer | M13 forward | M13 forward sequencing primer | 536 | 553 |
| primer | M13 reverse | M13 reverse sequencing primer | 3020 | 3040 |
| recombination site | attP1 | attP recombination site 1 present in empty vector with death cassette, replaced by attL1 in recombined form with gene of interest or other insert | 569 | 652 |
| recombination site | attP2 | attP recombination site 2 present in empty vector with death cassette, replaced by attL2 in recombined form with gene of interest or other insert | 2896 | 2983 |
| selectable marker | SpectR | spectinomycin resistance gene | 3471 | 4262 |
| trxn termination sequence | rrnB | rrnB T1 and T2 terminator | 268 | 470 |
- Gene
- Clone
- Organism
Clone
| Cloning Information : | 765053 |
|---|---|
| XGC Gene Symbol : | acta1 |
| Xenbase ORF Name : | actc1 ORF1 |
Organism
| Species Specific ID: | 379752 |
|---|
