anxa11 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTCGTTTCCCCCACAGAGTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGCTACCCAGGATAC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712442
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1506nts
Open reading frame: 1 to 1506

ATGAGCTACCCAGGATACCCGGGTGGGTTTCCACCAGTGCCCACAGGTAGTAACCCATGG
GGAGCTCCTGTTAACCCTCCTCCTATTGGGATGGATAACATGTCAAACTTCCCTCAGCAA
TACAACCCCCAGCAGTACATGACTTCAGGGTACCCGCCATCTGGTTTTGGAGGAATGGGC
CAACAACAGGGAATGTACCCTCAAGCTCCAGGTGGAGGCTACCCAGCACCTCCAGGTGGA
TATGGAGCACCGCCAATGCCTGGGCAACCTCAAGTTCCTTCCTATGGCATGTATCCTCCT
CCAGTAGCAGGCCAACAGCCAGGAATGCCTGGGTATAATACAGGATACCCAGTGGCACCT
CTGCCAGGCCAACCCATGCCAACACCCAGCCAGCCCCAGATGCCCATGCCTGGACAACCC
CCAGTGACATATCCTGGACAGCAGCCTACACCAAGTTATCCTCAAGGCCCAGCAATGAAT
CCATCAGGGCCCTCTTACCCATCTCCTGGTATGCCACCTTCAACAACGCCTACTGTCACA
CCTTCATATGGAAAGGTGAAACGAGGAACCATCACAGATGCTCCAAACTTTGATGCACTG
AGAGATGCTGAGGTGCTTAGGAAGGCCATGAAAGGCTTTGGAACTGATGAGCAGGCCATT
ATTGAGTGCCTGGGGAATCGTTCTAACAAACAAAGGCAACAGATCAGCCTCTCTTTCAAA
ACTGCCTATGGAAAGGATTTAATAAAGGATTTAAAATCCGAGCTCTCTGGAAATTTCGAG
AAGGCAATCCTGGCAATGATGAAATCTCCAACTCTTTTCGATGCAAATGAAATACGTGAG
GCAATTAAGGGTGCTGGCACAGATGAAGAATGTCTCATTGAGATTCTGGCTTCTAGGAGC
AATGCTGAGATACATGAAATCTGTGCTTTGTATAAAACAGAATATAAGAAAACTTTGGAA
CAAGCTATTAAAAGCGATACTTCTGGTCACTTCGAACGGCTGCTAATTTCACTGGCTCAG
GGAAATCGAGATGAAAGCAACAATGTAGACATGGCATTAGTCCAACGAGATGTACAGGAG
CTGTATGCTGCTGGAGAGAATCGCCTGGGGACTGATGAGTCCAAATTTAATGCCATCCTA
TGTGCACGCAGCAGGGCACACCTTAATGCAGTATTTTCAGAATACCAGCGCATGTGCAAT
CGGGATATTGAGAAAAGCATCTGTCGTGAGATGTCTGGGAACCTTGAAAGTGGCATGCTG
GCAGTGGTAAAGTGCCTAAAAAATACACCTGCCTTCTTCGCCGAGCGTCTCTACAAATCA
ATGAAGGGTGCTGGCACAAAGGACAAGACTCTTATCCGTATTCTGGTGTCCCGTAGTGAG
GTAGACTTGCTTGATATCCGTGTGGAATATAAAAGGATGTATGGCAAATCCCTTTACACA
GATATCACGGGAGACACATCCGGAGACTACAGGAAGATTCTGCTAAAACTCTGTGGGGGA
AACGACTAA

Protein sequence predicted based on nucleotide sequence

MSYPGYPGGFPPVPTGSNPWGAPVNPPPIGMDNMSNFPQQYNPQQYMTSGYPPSGFGGMG
QQQGMYPQAPGGGYPAPPGGYGAPPMPGQPQVPSYGMYPPPVAGQQPGMPGYNTGYPVAP
LPGQPMPTPSQPQMPMPGQPPVTYPGQQPTPSYPQGPAMNPSGPSYPSPGMPPSTTPTVT
PSYGKVKRGTITDAPNFDALRDAEVLRKAMKGFGTDEQAIIECLGNRSNKQRQQISLSFK
TAYGKDLIKDLKSELSGNFEKAILAMMKSPTLFDANEIREAIKGAGTDEECLIEILASRS
NAEIHEICALYKTEYKKTLEQAIKSDTSGHFERLLISLAQGNRDESNNVDMALVQRDVQE
LYAAGENRLGTDESKFNAILCARSRAHLNAVFSEYQRMCNRDIEKSICREMSGNLESGML
AVVKCLKNTPAFFAERLYKSMKGAGTKDKTLIRILVSRSEVDLLDIRVEYKRMYGKSLYT
DITGDTSGDYRKILLKLCGGND

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable