Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATTTAGCGTCCCTCTGCAGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTCTTTCAAGGAGGCCAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712457
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5814nts
Open reading frame: 1 to 5814
ATGTCTTTCAAGGAGGCCAAGCCTGGGGAGAGAGGCAAGAACCCAGAAGACCATGGCAGA
AAGCAGGCTGCAAGCTGGATGAACGGCATGGAGGCGGCCAACCAGCCCAGCACTTCGGCC
GAGAAGAAGAGTCACCACTGGCGGAGTTACAAGTTGATCATTGACCCGGCTCTGCGGAAG
GGGCAACAAAAACTCTATCGTTACGATGGTCTCAGCTTCAGCATGCCTAACTCCGGGGCC
CCCCCAGTGGATAGCGTTAGAGATCCCCGGATCGGACGTATATGGACCAAAACGAAAGAG
CTGGATTTGCCTGTCCCCAAACTCAAGATCGATGAGTTTTATGTAGGACCAGTGCCCCCC
AAGCAAGTGACGTTCGCTAAACTCAACGATAACATCCGCGAAAACTTCCTGGGGGACATG
TGCAAGAAATACGGCGAGGTGGAAGAGGTGGAGATCCTGTACAACCCTAAAAACAAGAAA
CACCTGGGCATTGCCAAAGTCATATTCGCCACCGTGAAAGGCGCCAAGGACGCCGTGAAA
CATTTGCACAACACCTCGGTCATGGGCAACATCATTCACGCGGAGCTGGACACGAAAGGT
GAAACGCGTATGCGATTCTATGAACTTTTGGTTAATGGTTATTATACTCCTCAGACTCTT
CCAGTTGGAAGTGATCTGGATGCCTCTCCTACTGTGAATGAAACCCCACAGGTTGTTGAG
CCAGTGAAACGTACAAAAGAGACTGCCGTTGGTGCATCAGTTACCCCAAATAGCAGTACT
CCTTTTTCCCATGATACAGCCTATTCAAGCTCTCGGCAAGGTACGCCTAATTCTTACAGC
CAGTTTACACCACAGTCGCAAGGGACTCCTCATACTCCCCGCTTGGGTACCCCTTTCTCA
CAGGATTCCAGTTATTCCAGTCGTCAAACGACACCAGCTTTTCATTATGGACAGGACTCT
AGTTTTAAACCAAGGAGACATGAAAATAAGTTTACAGACGCTTATAATAGAAGGCCAGGC
CACCACTATGTGCACAGTTCTGGGTCCTACCGGGGGCCTGAAAACACATTTAATGTGACC
AGACCCCAAGCAGAAACCGTGCAGTTTCCCCGAACTCCACCCTTGAGTCATTCTAGTGGG
AATAATAAATCCGCATTTTCACCTTATCAAGGAAGTACTGTTTTTCCTCAAACGGATGAC
AACCAGTATCCGCAGACATCAAGGGATATGGAGTACAGAAGGACAGGACCTCCGACTTCA
GATTCATATTCTGATGGCAGTTTAGAACTGAAACTAGTAAAAGAGAAACCAGAGGAACCT
CCACCTCCAGAGCCTGACTCTACCACAGAGCAAAAAGCATCATTTGCGCAGACCCCAGAG
AGGTGTGCGACACCTGGAACTCCAACTCTGGAGGCTGAAATGCAGCATGACAGTTTGGAC
ACACGGATTGCAATGCTATTAAAAGAGCAAAGAACCCAGCTGCATTTAATTTCTAGTGAT
CAAAATTCAGACAGTGAAATAAGGATGGAGGGAAGTCCAATATCATCCTCTTCCTCGCAG
CTTTCCCCTATACCTCCTTATAGTTCAAGCTCCCATTACCAGGATGTTACCCCTTCATCC
CGACCCTCAAGCACGGGATTAGAGGATATTAGCCCAACCCCTTTGCCAGATTCTGATGAC
GATGAGCCGATTCCTGGCACTGCCTCCCTTTGCCAGAACTCAAGGTCAGCATCTCCCATA
GACCAGATTAATCAGCCTGTGAGAAAAATGGAGACTCTGGATAACAAGGAACTTGTAGTG
GGTGATGAAACCCCAACATCTGAGAAAATGGATGAGGGTCATCCTTCCTCTGGAGAGGAC
ATGGAGATCTCCGATGATGAAGTTACCCCTTCTCCAATCACTAGTGCAGAGTGTGCCATA
ACTAGTTCATCTGTCATTCCTATACTACCACCAGGGTTTCCCCCTCTGCCGCCACCACCT
CCCCCACAGTCTGGATTTCCTATGCCGCCTCCTTTACCTCCTCCACCACCTCCTACCCAT
CCTTCAGTAACAGTACCCCCACCCCCATTGCCTGCACCACCTGGGGTGCCTCCACATCAC
ATATTGCATCATCCACCTCCATATCATCATTTCCCTGTGATGCAAGGGGAAATGATGAAT
GTTCTTGGGAACCATTGGGGTGGGATGACAATGTCCTTCCAAATGCAAACTCAAATGCTC
AGCCGCATGATGCAAGGGCAGGGTTCTTACCCCTACCACCATTTCATGGGAGGCTCCATG
CAGTTTGGTAACCAGCTACCTTATCGCCCCTTTGCTTTATCTGCACACCTTTCCAGAGGA
CAGCCTTGGCCACCCTTACCAAAATTTGATCCATCTGTACCACCACCAGGCTATGAGCCT
AAAAAGGAGGACCCTCACAAAGCAACAGTGGATGGAGTCTTGCAAGTCATAGTCAAGGAG
CTGAAAGCTATAATGAAAAGAGATTTGAACCGCAAGATGGTTGAGGTGGTGGCTTTTAGG
GCATTTGATGAATGGTGGGATAAAAAGGAACGTTTGGCAAAGCAGTCTTTAACACCTGTA
AAATCTGGAGAGAGCAAAGATGAAGATAAACAAAAGACTAAGGAGCATATAACCTCTAGC
CTCCTAGAAAGCTGGAATAAAGGGGAAGGATTAGGTTTTGAGGGCATTGGACTGGGCATT
GGTCTACGTGGTGCAATCCGACTTCCCTCTTTTAAGGTAAAGCGGAAAGAACCTCCTGAT
GCCGCACTGGCTGGAGATCAAAAGAGGATTCGCCCATCACATTCAGTAGATGATGAGGAT
GAAGAGTCTGAAAGAGATCGTGATATCTCGGGTACAGCATCTGATTTATCAAAGAAAGAT
GCGGATGCAGTAAACATCCGAAGAAGGCCAGCAAGACCCTTAGACAGTGAAGGGGAAGAA
GAGGTTGAAAGCGAAGGAGATGATGGGGAGACGTCAGATAAAGAAGCCTTTGAGAAAGAG
GACCAAGATGCTGGTTCTGTCTCTGCTTTGTCTAGTAAAAAACGGCTGTATGGAGAAAAA
GAGGACGAGGAGGATGAGACACAAAGCAGTGGTAAAGAAGAGGATCTTGTTTCAGAAGAA
GAAGATATCACCAGTGTTGCTTCCTCAAGAGCTGAGATGGATTCTTCTGATGAAAGTGAT
GAATCTTCAGAGTATGAGTCAAGCTCAGATTCAGATGACGAAATAGAGGAAGAAGATGAT
GATGATGAAGAAGAAGAGCTAGTTTTTGAGGATGATCAGTCTGAGGAACTAGATTTAGGT
CAGGAAGATTATATTGAAACTGATAGGGAAGAAGACTTCTTCAAGGAGGATGTATCTGAG
TGCAGCAGTCCAGTAAAGGCAGAGGCAGATATGGAATTAGAAGATGACGATGTGCAAAAA
TTAGAACAAGATGTTGCACACCAAACGGCACAAGATACATCACATCTTAGAAAGAAAGAC
TTGGATGTACCATTGGTGGAATCTAAAGAACACAAACAGGATACCTTTGATAAGATGGAG
AGGCTTTCTGCTGTACCCATGCAACAGAATGTTTTTAAAGAACACGAAAAAGCACCATCA
CCAATGAATGAAGAGGAAGAGTATATAGAGCTTCGTTTAGAACCTGTGCCTTTGGTTCCA
GACAATGCACCTCCAGCTGTACAAGAGCCTATGATCATCCGTCCCTTAACACCGACTGGT
GCTTTTGGTGAATCTGGCCCTGTTCTCAAACTTGAAGAACCCAAGCTGCAAGTTAATTTA
GCTCATTTTGTTGCAGAAGATGAAGACCTGTACCCTCGCACACCTGGCCGTGACACAGCT
GCTCATTCAGATACAGAGGTCACGTTTCAGCCTGGTTTGAAAGTGGCTCCATCCAGCCTT
CCTCTTTTGCAAAGTCACAATAAGGAAGAGGAGTGTTTGCTTCCCCCTGAAAAGCATACT
GGTCACTTAAAGGTGACTAAGACGTTGTCAGAGGAAGAACTACCCCGTACACCAGGCAGA
GATATTTTAGTTAAGTCCTCCCACCTTGGGAAGTCTCAGAGTACGGAGACCATTCCTGCA
ACTCCAGGTAGTGATGCTCCCCTCACAGGTAACAGCCTTACACTGACTTCTCCGCACATA
CCTGGCAGTCCTTTTTCTTATCTCTCCCAGTCACCAGGAATAATTAACAGTGGTATTCCA
AGGACACCTGGAAGAGATTTTAACTTCACCCCAATTTTCCCAGAATCAAATTCAATATTC
CCTTCCCATCCTTCTGGCAAGAAATCATCTGTAGATGAACCAGATGAAAAGTCATTTAAA
GAACCAACCAGTGCTTCCTTAACTATGAATAGTGTTCCTTCACCCATCCCATTTGCTAGC
CCGCCTAGAGGGGTTCCACACATGGACATAAGGTTAGAGACGGATGATCTGGAATCATCT
GATACTCAAGCATACCTTTCTGATAAATTATTAAGTGAGGAGAGCGAATGTGAGTTTACT
AAAGGGCAGCTCCCTTCCACTGATGAATCTGCACCCTCACCACCTTTCCCTCCTACAGAC
AAGAGGAAAGGACCCAAAAAACCTCTTGCTGCTCATGAATTTGAGGCTTGTGTTGCTTTG
TCAGAAGGTGCTTTGGGTAAACAATTGTTTATTGGACAACCTGATTCTGTCAGTGGTATT
AAGGATCCAGCAGCTGTGCCACTGGACTTTAGGAATGATGGCCTTTCTGAAAACACAGTT
CATGATCCCATTATTCAAAAAGTCCCTTTAAAAGAGTTGGAAAATCAGTGGAATGAAGTT
TTAAAAGAGGAGGAGGAGGATATAAGTAAACATAAAAAATCAAGGAACTCCAGGCTTAAC
AAACTATATGATGAGTTTTCAACTTTACCTTCTCCTGAATATTCCCCGCCCCGAGCTATG
TTTAAGCCTCGCTCTGAATTTGAGGAAATGACCATTCTGTATGATATATGGAATGGAGGA
ATAGATGATGAAGATATGAAATACATGTGCATAACCTATGACAGACTGTTACAGCAAGAT
AATGGCATGGACTGGCTCAATGACACATTATGGGTGTACCACCCTCCAACCAGTGTTTAT
TCCCCCAAGAAGAAGAAGCGGGATGATGGTTTACGGGAGCATGTAACAGGATGTGCCCGC
AGTGAGGGATATTATAAAATTGATAAGAAAGATAAACTTAAATACCTCATCAACAACCGT
TCTTTGGCTGACGAGCCTCCCATCGACACCCAGGGAAAGAGTATCCCAGCTCAGCCTCAA
GCCTCCACCCGAGCAGGCTCGGAAAGAAGGTCAGAACAGCGCAGACTGCTCTCTTCCTTC
ACTGGTAGCTGTGACAGTGACTTGCTCAAATTTAATCAGCTGAAGTTCCGAAAGAAAAAG
ATACGTTTCTGCAAGAGCCATATACATGACTGGGGTCTTTTTGCCATGGAACCAATTGTA
GCTGATGAGATGGTGATTGAGTATGTCGGACAAAATATTAGACAGGTAATTGCAGATATG
CGGGAAAAGCGATATGAGGATGAAGGAATCGGCAGCAGTTATATGTTTAGGGTGGACCAT
GACACTATCATTGATGCAACAAAATGTGGGAATTTTGCACGGTTCATTAACCACAGCTGT
AATCCTAATTGCTATGCAAAGGTTGTTACTGTGGAGTCTCAGAAGAAGATTGTGATCTAC
TCTAAACAGTATATCAATGTGAATGAAGAGATAACATATGATTACAAGTTCCCTATAGAG
GACGTGAAGATTCCCTGTCTTTGCGGAGCAGAGAACTGCAGAGGGACGCTAAATTAG
Protein sequence predicted based on nucleotide sequence
MSFKEAKPGERGKNPEDHGRKQAASWMNGMEAANQPSTSAEKKSHHWRSYKLIIDPALRK GQQKLYRYDGLSFSMPNSGAPPVDSVRDPRIGRIWTKTKELDLPVPKLKIDEFYVGPVPP KQVTFAKLNDNIRENFLGDMCKKYGEVEEVEILYNPKNKKHLGIAKVIFATVKGAKDAVK HLHNTSVMGNIIHAELDTKGETRMRFYELLVNGYYTPQTLPVGSDLDASPTVNETPQVVE PVKRTKETAVGASVTPNSSTPFSHDTAYSSSRQGTPNSYSQFTPQSQGTPHTPRLGTPFS QDSSYSSRQTTPAFHYGQDSSFKPRRHENKFTDAYNRRPGHHYVHSSGSYRGPENTFNVT RPQAETVQFPRTPPLSHSSGNNKSAFSPYQGSTVFPQTDDNQYPQTSRDMEYRRTGPPTS DSYSDGSLELKLVKEKPEEPPPPEPDSTTEQKASFAQTPERCATPGTPTLEAEMQHDSLD TRIAMLLKEQRTQLHLISSDQNSDSEIRMEGSPISSSSSQLSPIPPYSSSSHYQDVTPSS RPSSTGLEDISPTPLPDSDDDEPIPGTASLCQNSRSASPIDQINQPVRKMETLDNKELVV GDETPTSEKMDEGHPSSGEDMEISDDEVTPSPITSAECAITSSSVIPILPPGFPPLPPPP PPQSGFPMPPPLPPPPPPTHPSVTVPPPPLPAPPGVPPHHILHHPPPYHHFPVMQGEMMN VLGNHWGGMTMSFQMQTQMLSRMMQGQGSYPYHHFMGGSMQFGNQLPYRPFALSAHLSRG QPWPPLPKFDPSVPPPGYEPKKEDPHKATVDGVLQVIVKELKAIMKRDLNRKMVEVVAFR AFDEWWDKKERLAKQSLTPVKSGESKDEDKQKTKEHITSSLLESWNKGEGLGFEGIGLGI GLRGAIRLPSFKVKRKEPPDAALAGDQKRIRPSHSVDDEDEESERDRDISGTASDLSKKD ADAVNIRRRPARPLDSEGEEEVESEGDDGETSDKEAFEKEDQDAGSVSALSSKKRLYGEK EDEEDETQSSGKEEDLVSEEEDITSVASSRAEMDSSDESDESSEYESSSDSDDEIEEEDD DDEEEELVFEDDQSEELDLGQEDYIETDREEDFFKEDVSECSSPVKAEADMELEDDDVQK LEQDVAHQTAQDTSHLRKKDLDVPLVESKEHKQDTFDKMERLSAVPMQQNVFKEHEKAPS PMNEEEEYIELRLEPVPLVPDNAPPAVQEPMIIRPLTPTGAFGESGPVLKLEEPKLQVNL AHFVAEDEDLYPRTPGRDTAAHSDTEVTFQPGLKVAPSSLPLLQSHNKEEECLLPPEKHT GHLKVTKTLSEEELPRTPGRDILVKSSHLGKSQSTETIPATPGSDAPLTGNSLTLTSPHI PGSPFSYLSQSPGIINSGIPRTPGRDFNFTPIFPESNSIFPSHPSGKKSSVDEPDEKSFK EPTSASLTMNSVPSPIPFASPPRGVPHMDIRLETDDLESSDTQAYLSDKLLSEESECEFT KGQLPSTDESAPSPPFPPTDKRKGPKKPLAAHEFEACVALSEGALGKQLFIGQPDSVSGI KDPAAVPLDFRNDGLSENTVHDPIIQKVPLKELENQWNEVLKEEEEDISKHKKSRNSRLN KLYDEFSTLPSPEYSPPRAMFKPRSEFEEMTILYDIWNGGIDDEDMKYMCITYDRLLQQD NGMDWLNDTLWVYHPPTSVYSPKKKKRDDGLREHVTGCARSEGYYKIDKKDKLKYLINNR SLADEPPIDTQGKSIPAQPQASTRAGSERRSEQRRLLSSFTGSCDSDLLKFNQLKFRKKK IRFCKSHIHDWGLFAMEPIVADEMVIEYVGQNIRQVIADMREKRYEDEGIGSSYMFRVDH DTIIDATKCGNFARFINHSCNPNCYAKVVTVESQKKIVIYSKQYINVNEEITYDYKFPIE DVKIPCLCGAENCRGTLN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
