bzw1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATCACCCTCTTCAGTCTC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAATAATCAAAAGCAACAAAAG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712639
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1257nts
Open reading frame: 1 to 1257

ATGAATAATCAAAAGCAACAAAAGCCAACGCTATCTGGCCAGCGTTTTAAAACCCGAAAA
AGAGATGAAAAGGAGAGGTTTGATCCTACTCAATTCCAAGACTGCATCATTCAAGGCTTA
AATGAAACTGGTAACGATTTGGAAGCAGTAGCGAAGTTTCTTGATGCTTCAGGGGCAAAG
CTAGACTATCGGCGCTATGCAGAAACTCTCTTTGACATTCTGGTGGCTGGCGGAATGCTG
GCACCAGGCGGAACTCTGGCCGATGAGGTGATTCGTACTGATGTCTGTGTGTTTTCAGCA
CAAGAAGATCTTGAAACCATGCAGGCGTTTGCTCAGGTTTTTAACAAGTTAATCAGACGC
TACAAGTACCTGGAGAAAGGCTTTGAAGAAGAGGTCAAAAAGCTGTTGCTGTTTCTGAAA
GGTTTTTCAGAATCTGAGAGGAATAAGCTGGCTATGCTCACTGGTATTCTTTTGGCCAAT
GGGAATCTTTCTGCATCAATACTTAACAGTCTCTACAATGAAAATTTGGTCAAAGAAGGT
GTTTCTGCAGCATTTGCAGTAAAACTGTTTAAATCATGGATCAATGAAAAAGATATCAAT
GCTGTTGCTGGCAGTCTCCGAAAAGTGAACATGGATAACCGGTTAATGGAACTTTTTCCT
GCCAACAAGCAAACTGTGGAACATTTCACTAAATACTATACTGATGCAGGACTAAAAGAA
TTGGCAGAATATGTTCGGAACCAGCAAACAATTGGCGCTCGCAAAGAGATCCATAAAGAG
CTTCAGGAAATGATTTCACGTGGAGAAGCTCATAAGGAGATAAGTGTGTACGTGAAGGAT
GAAATGAAGAAAAACAATATTTCTGAGCAAACTGTTATTGGTATTCTATGGTCCAGTATT
ATGAGCTGCGTGGAATGGAACAAAAAGGAGGAGCTTGTTACAGAGCAAGCCATCAAACAC
TTGAAGCAATACAGCCCTCTGCTTGCTGCCTTTACTACACAAGGTCAGTCAGAACTGACT
TTGCTGCTGAAAATTCAGGAGTATTGTTATGACAACATTCACTTCATGAAATCCTTCCAG
AAAATAGTAGTGCTCTTTTATAAAGCTGAAGTTCTGAGTGAAGAGCCCATTCTGAAGTGG
TACAAAGATGCACATGTTGCAAAAGGCAAAAGTGTGTTCCTGGAGCAAATGAAAAAATTT
GTTGAATGGCTTAAAAATGCAGAAGAAGAATCGGAATCAGAGACTGAAGAGGGTGATTGA

Protein sequence predicted based on nucleotide sequence

MNNQKQQKPTLSGQRFKTRKRDEKERFDPTQFQDCIIQGLNETGNDLEAVAKFLDASGAK
LDYRRYAETLFDILVAGGMLAPGGTLADEVIRTDVCVFSAQEDLETMQAFAQVFNKLIRR
YKYLEKGFEEEVKKLLLFLKGFSESERNKLAMLTGILLANGNLSASILNSLYNENLVKEG
VSAAFAVKLFKSWINEKDINAVAGSLRKVNMDNRLMELFPANKQTVEHFTKYYTDAGLKE
LAEYVRNQQTIGARKEIHKELQEMISRGEAHKEISVYVKDEMKKNNISEQTVIGILWSSI
MSCVEWNKKEELVTEQAIKHLKQYSPLLAAFTTQGQSELTLLLKIQEYCYDNIHFMKSFQ
KIVVLFYKAEVLSEEPILKWYKDAHVAKGKSVFLEQMKKFVEWLKNAEEESESETEEGD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable