pus10 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTCGTCCAGGGAAGGAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCTTCTTATTGAAGAAAAATAT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712783
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1545nts
Open reading frame: 1 to 1545

ATGCTTCTTATTGAAGAAAAATATAGATCGGTTGCCCAGGTGTTACTCTCCTCTGGTACC
TGCTCACGTTGTGTCTTACGCTTCTGCTGTGTTGGGTCTCCAGCCAATTACAGGCTTCCC
TGCAAGGAGGTAACTTATGAGCTGCAGAAGTATCTCTCACACGGTGATCCAGCTGAAGAG
AATGATACTCCACCTAGCAAGAAAGCCAAGATTGAAGAAGATACCTCCTCCAATGAACAT
TTGGGAAACTGTGAGGATGTTAATGGTTCTCAGGTTGTGAGAATCTGCCCTTTGTGCTTA
GGAATACTTCAACAGTTCTGTGAACCAGAGTTTATTGAAAAGGTATTTGTGAAAATAAAT
TCCGCAGTCTATGAATTGAAAGATTTTGTTTTATCCATCTCATTACCTGCGCAGCTTTCT
GTCCGAGAGCATTCGGCGTGGCTTCAAGCAAAACAGGAAATGGGAAAACATGGACATTCC
CTGGATAAAACGGACATTGTACAGCTAAAGGAAGCCTACAAATGGATTATCCATCCTCTG
CTATCGGATCAACTGGGGATTCAAGCAGATAGCAAGAGTTTGTTTGAAGTAGCCGTAGTC
TTCACTCACCCTGAAACTGATGGAGATTGTCACTTCTTAGCCACAGTCTGTCGAGATTGT
TTCAAACCTACTAAGAATAAACAGTCAGTTTTCACCAGAATGGCAGTTGTGAAGGCGCTA
GAGAAGATAAAAGAAGAAGATTTCCGTGTCCAGTTTCCATTCCCTCCAAGTTCTCCAGAG
ACTACCTGTGAAGTTGTGGATATCCAGTGCAACCATTCTCCCGTTTTTGTCGCTGGTAGG
TATAACAAATACTCAAGGAATTTACCGCAAACCCCTTGGATTATTGATGGAGAAAGGAAA
ATAGAATCATCTGTAGAAGAGCTGATAACAGATCATCTGGTGGCAGCATTTAGATCGTCC
AGTTTCAACTTTTCATCGTCTGGAAGGGAAGATGTGGACGTCAGAACATTAGGCAAAGGA
AGACCATTTGCAATAGAGCTTCTAAACCCTCACAAAGTCCAGTTCACGGGGCAGGAGATA
AAGGCACTGCAGCAGAAAATAAATACCTCCGACAAAATCAAAGTACGAGATTTGCAGATT
GTGTCCAGGGAGGCTGTGGCACATATGAAGGAAGGAGAAGAGGAAAAAACTAAATGTTAT
TGTGCTCTAATATGGATTGAGAAAATGGTCCGAAATGAAGATCTTCAACTGCTTGATGGA
TTGGAGGAGCTGACGATTGCTCAGAAAACCCCCCTTCGTGTCCTTCACCGCAGGCCACTT
GCTTCAAGGTCACGTACAATCCACACCATGAGAACCGAGTATGTGGATGAACATCACTTC
CGGCTCTATTTAAAGACCCAAGCAGGAACTTACATTAAAGAATTTGTCCATGGAGATTTT
GGAAGAACAAAACCAAACGTAGGATCTATAATGGAAACCAATGCGGATATTTTGGAACTG
GACGTGGAGTCTGTTGATGTCGATTGGCCTCCTTCCCTGGACGACTGA

Protein sequence predicted based on nucleotide sequence

MLLIEEKYRSVAQVLLSSGTCSRCVLRFCCVGSPANYRLPCKEVTYELQKYLSHGDPAEE
NDTPPSKKAKIEEDTSSNEHLGNCEDVNGSQVVRICPLCLGILQQFCEPEFIEKVFVKIN
SAVYELKDFVLSISLPAQLSVREHSAWLQAKQEMGKHGHSLDKTDIVQLKEAYKWIIHPL
LSDQLGIQADSKSLFEVAVVFTHPETDGDCHFLATVCRDCFKPTKNKQSVFTRMAVVKAL
EKIKEEDFRVQFPFPPSSPETTCEVVDIQCNHSPVFVAGRYNKYSRNLPQTPWIIDGERK
IESSVEELITDHLVAAFRSSSFNFSSSGREDVDVRTLGKGRPFAIELLNPHKVQFTGQEI
KALQQKINTSDKIKVRDLQIVSREAVAHMKEGEEEKTKCYCALIWIEKMVRNEDLQLLDG
LEELTIAQKTPLRVLHRRPLASRSRTIHTMRTEYVDEHHFRLYLKTQAGTYIKEFVHGDF
GRTKPNVGSIMETNADILELDVESVDVDWPPSLDD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable