copb1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATATACAGGATTTCTTCTGCGAAAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACTGCCGCTGAGAAT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712934
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2880nts
Open reading frame: 1 to 2880

ATGACTGCCGCTGAGAATGTGTGTTACACTCTCATTAACGTCCCAATGGACTCGGAACCT
CCATCTGAAATTACTCTGAAGGCAGATCTGGAGAAAGGAGATGCGAAGTCCAAGACGGAG
GCCTTGAAGAAAGTGATCATCATGATCTTGAATGGAGAGAAGCTTCCGGGCCTTCTGATG
ACCATCATCCGCTTTGTGTTGCCGCTGCAGGATCACACCATCAAGAAGCTTCTCTTGGTC
TTTTGGGAGATTGTGCCCAAAACAACACCCGATGGGAAACTATTGCAGGAGATGATCCTG
GTGTGTGATGCCTACAGAAAGGATCTCCAGCACCCCAATGAGTTCATCAGGGGATCTACA
CTGCGATTCTTGTGCAAACTGAAGGAGGCAGAACTTCTGGAGCCTCTGATGCCTGCGATT
CGGGCCTGCCTGGACCACCGGCACAGCTATGTGCGTAGGAACGCAGTGCTCGCAATCTAC
ACCATCTACAGAAACTTTGAGCACCTGATCCCAGACGCTCCAGAACTGATCCATGATTTC
CTGGTGAATGAGAAAGATGCGAGCTGCAAGAGGAATGCCTTCATGATGCTCATCCATGCC
GACCAGGACCGGGCCCTGGATTACCTGAGCACCTGCATTGACCAAGTGCAGACATTTGGG
GACATCCTGCAGTTGGTCATCGTTGAGCTGATATACAAGGTGTGCCATGCTAACCCTTCT
GAGAGAGCTCGCTTCATCCGCTGCATCTACAACCTTCTCCAGTCCTCCAGCCCCGCTGTC
AGGTATGAAGCTGCCGGAACACTGGTCACCCTCTCCAGCGCTCCAACTGCCGTCAAGGCA
GCTGCTCAGTGCTACATTGACTTGATCATCAAGGAGAGCGACAACAACGTGAAGCTAATT
GTGTTGGACCGACTTGTGGAGCTAAAGGACCATCCTTCCCATGAGCGAGTGCTGCAGGAT
CTGGTCATGGACATCCTGCGGGTGCTGAGCACCCCTGATCTGGAAGTGCGCAAGAAAACC
CTCCAGCTGGCTCTTGAATTGGTTTCCTCACGCAATGTGGAAGAGCTGGTCATCGTGCTG
AAGAAGGAGGTGATCAAGACCAATAACGTGACGGAGCACGAGGACACGGACAAGTACAGG
CAACTGCTGGTGAGGACTCTCCACTCGTGCAGCGTGAGATTCCCAGATATGGCTGCCAAC
GTCATTCCCGTGCTCATGGAGTTCCTCAGCGACAGCAGTGAGGCGGCGTCGGCTGATGTC
CTGGAGTTCGTACGCGAGGCCGTGCAGCGATTCGATCATCTCCGGCCGCTGATAGTCGAG
AAGATGCTGGAGGTTTTCCATGCCATCAAATCAGTCAAGATATACAGGGGGGCCCTGTGG
ATTATGGGTGAATACTGCAGCACTAAAGAAGATATTAACAGTGTGATGACTGAGGTGCGC
AGGTCACTGGGAGAGGTGCCGATTGTAGAGACAGAACTCAAGAAGGAATCGGGAGAAGTA
AAAGCAGAGGAAGATGTGAGTGTGGGGCCGGCGCAGAAGCTGGTGACAGAAATGGGCACT
TACGTTACACAGAGCGCCCTCAGCAGCTCACGGCCGGCCAAGAAAGAGGAGGAGAGTTCC
ACTGTTGCTCCTTACAGACCTCCCCTCAGAGGGTTCCTGCTGGACGGCGATTTCTACGTT
GCCGCATCTCTCGCCACCACCCTGACAAAGATTGCCCTGCGCTACGTGGCTTTGGTCCAG
GAGAAGAGGAAACAAAATTCATTTGTCGCGGAAGCCATGTTGCTAATGGCCACCATCCTT
CACCTGGGCAAGTCCTCCCTCCCCAAGAAGCCAATCACAGACGACGATGTGGATCGCATC
TCCCTGTGCCTCAAGGTGCTGTCGGAATGTTCTCCCCTCATGAACGAGATCTTCAACAAG
GAATGTCGCCAGTCCCTGTCCCACATGTTGTCGGCCAAGCTGGAGGAGGAGAAGCTCTCC
CAGAAGAAAGAGTCAGAGAAGAGGAACGTGACTGTTCAAGCAGACGACCCCATATCCTTC
ATGCAACTGACGGCCAAGAACGAAACGGCCTCCAAAGAGGATCAGTTTCAGCTCAGCCTT
CTGGCCGCCATGGGGACAACGCAAAGGAAGGAGGCCGCCGACCCTCTGGCCTCCAAACTC
AATAAGGTGACGCAGCTGACGGGATTCTCAGACCCAGTGTACGCAGAAGCTTATGTGCAT
GTCAATCAATATGATATTGTGCTGGATGTGCTGGTGGTGAATCAGACCGGCGACACCCTG
CAGAACTGTACCCTCGAGCTGGCAACCCTAGGTGATCTGAAGCTCGTAGAGAAGCCCTCC
CCTCTCACCCTGGCCCCCCACGACTTTGCCAACATTAAGGCTAACGTCAAAGTCGCCTCT
ACGGAGAACGGAATCATTTTTGGCAATATCGTGTACGACGTGTCGGGCGCAGCCAGCGAC
AGGAACTGTGTCGTCCTCAGTGATATCCACATCGATATTATGGACTACATCCAGCCTGCG
TCCTGCTCCGACACGGAATTCCGACAGATGTGGGCGGAGTTTGAATGGGAGAATAAGGTG
ACGGTTAATACAAACATCACAGATCTGAATGAATATCTGCAACACATTCTGAAATCTACA
AACATGAAATGTCTCACCCCAGAGAAGGCGCTGTCTGGTTACTGCGGCTTCATGGCGGCC
AATCTTTACGCCCGCTCCATATTTGGCGAAGACGCCCTCGCCAACGTCAGCATCGAGAAG
CCCATTCACCTCGGACCGGAAGCCCCCGTAACTGGGCACATAAGGATTCGAGCCAAGAGC
CAGGGGATGGCGCTGAGCCTCGGGGATAAGATCAACCTTTCGCAGAAGAAATCCTGTATA
TAA

Protein sequence predicted based on nucleotide sequence

MTAAENVCYTLINVPMDSEPPSEITLKADLEKGDAKSKTEALKKVIIMILNGEKLPGLLM
TIIRFVLPLQDHTIKKLLLVFWEIVPKTTPDGKLLQEMILVCDAYRKDLQHPNEFIRGST
LRFLCKLKEAELLEPLMPAIRACLDHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDF
LVNEKDASCKRNAFMMLIHADQDRALDYLSTCIDQVQTFGDILQLVIVELIYKVCHANPS
ERARFIRCIYNLLQSSSPAVRYEAAGTLVTLSSAPTAVKAAAQCYIDLIIKESDNNVKLI
VLDRLVELKDHPSHERVLQDLVMDILRVLSTPDLEVRKKTLQLALELVSSRNVEELVIVL
KKEVIKTNNVTEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDSSEAASADV
LEFVREAVQRFDHLRPLIVEKMLEVFHAIKSVKIYRGALWIMGEYCSTKEDINSVMTEVR
RSLGEVPIVETELKKESGEVKAEEDVSVGPAQKLVTEMGTYVTQSALSSSRPAKKEEESS
TVAPYRPPLRGFLLDGDFYVAASLATTLTKIALRYVALVQEKRKQNSFVAEAMLLMATIL
HLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNEIFNKECRQSLSHMLSAKLEEEKLS
QKKESEKRNVTVQADDPISFMQLTAKNETASKEDQFQLSLLAAMGTTQRKEAADPLASKL
NKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTGDTLQNCTLELATLGDLKLVEKPS
PLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPA
SCSDTEFRQMWAEFEWENKVTVNTNITDLNEYLQHILKSTNMKCLTPEKALSGYCGFMAA
NLYARSIFGEDALANVSIEKPIHLGPEAPVTGHIRIRAKSQGMALSLGDKINLSQKKSCI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable