glud1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATGTGAAAGTCAGGCCAGCCTCAT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTACCGATACATAGGGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712952
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1620nts
Open reading frame: 1 to 1620

ATGTACCGATACATAGGGGAGTTGGTATCTCGCGGAGGGGGTGCTCTGGCTTCATGCACT
GCTGATAGTGTCTTGCCGCTTTCTGCCGCACCCATTCTGCGTCGCTACAGCCAGGCCGTG
AACGGAGACGATGACCCCAACTTCTTCAAGATGGTGGAGGGATTCTTCGACAGAGGCGCC
GGCATTGTGGAGGACAAGTTGGTGGAGGACTTGCGGACACGCGAGACGGAGGAGCAGAAG
AGGCTGCGGGTGAGGGGGATCCTGCGCATCATAAAACCCTGCAACCACGTCCTGAGTGTC
AGCTTCCCTATCAAAAGGGACAATGGCGAGTGGGAGGTTATCGAGGGCTACCGGGCACAG
CACAGCCAGCACCGGACTCCGTGCAAGGGAGGTATTCGCTACAGCACAGAAGTCAGTGTT
GATGAAGTAAAAGCCTTGGCATCACTGATGACCTATAAATGTGCAGTAGTTGATGTGCCT
TTTGGGGGAGCCAAAGCTGGAGTCAAAATTAACCCAAGAAATTTTTCTGATGCAGAATTG
GAAAAGATAACTAGAAGGTTTACAATAGAACTCGCAAAGAAGGGCTTTATTGGACCAGGC
ATAGATGTTCCTGCTCCAGATATGAGTACAGGTGAACGAGAAATGTCATGGATTGCTGAT
ACTTATGCTAATACAATTGGCCACACGGATATCAATGCTCATGCTTGTGTAACTGGAAAA
CCCATCAGCCAAGGAGGTATTCATGGTCGTATATCTGCAACTGGTCGTGGTGTCTTCCAT
GGCATTGAAAACTTTATCAATGAGGCTTCCTACATGAGTCAACTGGGTATGACTCCTGGG
TTTGGTGACAAGACTTTCGTCATTCAGGGCTTTGGAAATGTAGGTCTTCACTCTATGCGA
TATCTTCACCGATTTGGTGCCAAATGTGTTGGAATTGGTGAAATTGATGGGACCATCTGG
AACCCAAATGGCATTGACCCTAAAGAACTCGAGGACTACAAACTACAACATGGGACAATT
GTTGGGTTTCCAAAGGCCCAGCCATATGATGGAAACATTTTAGAGGCAGATTGTGACATT
CTTATCCCAGCTGCTAGTGAGAAGCAACTGACAAAGTCTAATGCACATAAGATAAAGGCT
AAGATTATTGCAGAAGGAGCCAATGGTCCTACAACCCCAGAAGCTGACAAAATCTTCCTT
GAAAGAAACATCATGGTCATTCCAGATCTATATTTGAATGCTGGTGGTGTCACGGTTTCT
TATTTTGAGTGGCTTAAAAATCTCAATCATGTTAGCTATGGACGTTTGACCTTCAAATAT
GAGCGGGACTCAAACTACCATTTGCTCATGTCTGTCCAAGAGAGCTTAGAAAGAAAGTTT
GGGAAACATGGTGGGGCCATCCCAGTTGTGCCCACAGCAGAATTCCAGGCTAGGATATCT
GGTGCGTCTGAGAAAGATATTGTCCATTCTGGCTTGGCGTATACAATGGAACGGTCTGCA
AGGCAAATCATGCGCACTGCTATGAAGTACAACCTTGGTTTGGACCTAAGAACTGCTGCC
TACGTAAATGCCATAGAAAAAGTATTTAAGGTGTACAATGAGGCTGGCCTGACTTTCACA
TAA

Protein sequence predicted based on nucleotide sequence

MYRYIGELVSRGGGALASCTADSVLPLSAAPILRRYSQAVNGDDDPNFFKMVEGFFDRGA
GIVEDKLVEDLRTRETEEQKRLRVRGILRIIKPCNHVLSVSFPIKRDNGEWEVIEGYRAQ
HSQHRTPCKGGIRYSTEVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPRNFSDAEL
EKITRRFTIELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYANTIGHTDINAHACVTGK
PISQGGIHGRISATGRGVFHGIENFINEASYMSQLGMTPGFGDKTFVIQGFGNVGLHSMR
YLHRFGAKCVGIGEIDGTIWNPNGIDPKELEDYKLQHGTIVGFPKAQPYDGNILEADCDI
LIPAASEKQLTKSNAHKIKAKIIAEGANGPTTPEADKIFLERNIMVIPDLYLNAGGVTVS
YFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGAIPVVPTAEFQARIS
GASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGLTFT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable