efcab7 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGATAAGATATTTTGCACAC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTAACCACTCATCTCT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712983
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1860nts
Open reading frame: 1 to 1860

ATGGCTAACCACTCATCTCTACCGAGTCAAAAATATGCAGCCAGTGAACGTCAAGAGTAT
CAAAAACCACAGCAGAATGAGGAAGAAATCTTCTACTCAGTTAGCCGTGCTGCCTATCTT
ACTGTCTTTAAAAGCAGTCTAGACAACATTACGACCAAAGATCAGCTGCAATTAGTGCTC
CAGCAAACTGGTCGAAATCCATCCAACAGAGTACTTAACAAGTATTGGACTCCACGGACC
AAAGAGTTAAATTTTGATGATTTCTGTGCCATCCTAAAAAAGGAGAAACCTGCTACCAAA
AATGAGCTTCTGAAAGCTTTTAGGAAAATAGATACAAATAACAAAGGATATATATTACAC
AATGACTTGTATGAAATCCTTACCACAAAAGGTGAGAAAATGTCTCAAGAGGAAGTCAAC
TCCGTGTTTCGTTTAGCTGAAGTAAACAGCAATGGCAAGCTTGACTACAATAAGTTTTGT
AGCACATTCTTTAAAACCTGTGAACAGTGTGCTAAGGTAGCATCAGAGAGAATGGACAGC
AATAGCAAAGCAAAACGACAGCAGTTTGGAAGCTATATCGAAAAGTCACCAGAGCGTTCC
TCGTCACCAAAATCATCGCATGGAAATTTAAAGCTTTTTGATTCTGAAACTTCTACAAGG
AAAGAAAATAAATCTTCTAGGCCCTCTTCAGCTCGTAGCTACAAAGCTACAATGTCTACA
GTAATTAATATGGGCATACCTGGGACCAGAACTGCCAAGCTCATTGAGCCTAATAATTTA
AAGGACTGGCATACCACATCAACCAAAGGATGCTTTTTTCTGGAGGACAATGGTGATATA
ATCAGCCATCATTACAAATTACAAGTTTCTGAAAAGTCAACAGTTTATCTTACAATTAAA
CCTTTAAACCTCAGCAAAGTTGAAGGAAAACCTTCCCCATGGATGTCCGTTGACACTTCC
CTGTTTATTCTTAAGGAAAATAATGGCCGAGCAGACCTGGTTTCTTTCACAGAGCTCAGG
AACCAAGAGACCTCTGGATGGAAAGGGGAGCTTGGAGTTGGTGTTTACTGGCTTATTCCA
TTTACAACAGGGTGTAGACTGAGGAAGAAAAAAAAACAAACCATCAAAGAAGCTCGGTTG
GTGTACAGAGATGGAAACGAAGATTTAGTTCTCACGCCGGAATTTAAATCTGCTTTATCA
GACATGTTTGACATAATTGATTTAGATGGAAATGGTCTTCTGAGCCTGGCAGAGTATAAT
TTCTTTGAGATGAGAACCAGTGGTGAAAAATGTGATCTTGATGCCTGGGAGGTGTGCAAA
GAAAATTTTGAAACAAAGAAAAACGAGTTAACAAGGCAGGGGTTTATGGAGCTAAATCTC
ATGGAGGCGAACGACCGGGAAGGAGATCCCAGTGACCTGTGGGTAACACTGCAAACCATG
GGGTACAACAAAGCACTTGAAATGACTGAGGCATGCCCGTTTGTTATTGAAGTCCATGCT
GAAAAATGCAGCCCACGGCTGAAAGCTGTAAGCCTGGAATCCAGCAATAAGCAGCTGCAG
GGGGCAGTTTGTAAATCGGTCAAGCTCAAAGGGGATGCAAAGCCTATGGATCGTTATGAA
GATGTCATTGTCTACACATACAAAAATGATACCCATGTCACATCAGTGATGGAAAATAAG
TCTGATGAGAAACTGATTTTGCAAGTCAACAATGAACAATGTAAAAACTGCATCAGCAGC
CGAGGATTGCAAGTGTTCGCTGTGGAGCTGCTACCAAAATCAGTCATGGTCTGTCAACAT
GTCATGCCACTGAATGAAAAACAAGAGTGGATGTACAATTGTGTGCAAAATATCTTATCC
TAA

Protein sequence predicted based on nucleotide sequence

MANHSSLPSQKYAASERQEYQKPQQNEEEIFYSVSRAAYLTVFKSSLDNITTKDQLQLVL
QQTGRNPSNRVLNKYWTPRTKELNFDDFCAILKKEKPATKNELLKAFRKIDTNNKGYILH
NDLYEILTTKGEKMSQEEVNSVFRLAEVNSNGKLDYNKFCSTFFKTCEQCAKVASERMDS
NSKAKRQQFGSYIEKSPERSSSPKSSHGNLKLFDSETSTRKENKSSRPSSARSYKATMST
VINMGIPGTRTAKLIEPNNLKDWHTTSTKGCFFLEDNGDIISHHYKLQVSEKSTVYLTIK
PLNLSKVEGKPSPWMSVDTSLFILKENNGRADLVSFTELRNQETSGWKGELGVGVYWLIP
FTTGCRLRKKKKQTIKEARLVYRDGNEDLVLTPEFKSALSDMFDIIDLDGNGLLSLAEYN
FFEMRTSGEKCDLDAWEVCKENFETKKNELTRQGFMELNLMEANDREGDPSDLWVTLQTM
GYNKALEMTEACPFVIEVHAEKCSPRLKAVSLESSNKQLQGAVCKSVKLKGDAKPMDRYE
DVIVYTYKNDTHVTSVMENKSDEKLILQVNNEQCKNCISSRGLQVFAVELLPKSVMVCQH
VMPLNEKQEWMYNCVQNILS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable