cybb (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAAGTTCTCTTTGTTGAAGATGAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGCAACTGGATTGTAAATGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713128
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1710nts
Open reading frame: 1 to 1710

ATGGGCAACTGGATTGTAAATGAAGGCCTCTCCATCGCTGTTATTATTGTCTGGCTTGCC
CTAAATGGTTACCTGTTTTGGAATTTTTACCTTGTCTATGACGAGGGCGAAGCATACTTC
TATTCTAGAAAACTTTATGGGTCCGCCCTTGCCTGGGCAAGAGCTCCAGCAGCCTGCCTG
AACTTCAACTGCCTGCTTATCCTTCTACCAGTGTGTCGAAATCTACTCTCTTTTCTGAGA
GGGTCAAGTGCGTGTTGTGGTAGAAGCCTTAGACGCCAGCTGGATAGAAACCTTACCTTT
CATAAGATGGTGGCTTGGATGATTGCACTTCACACAGCTATCCATACAGGGGCGCACTTG
TTTAATGTGGAAAGACTGGTGGACGCACGTGTGGAGGCAAACGGAACCATCCAGGCAGCT
CTCACATCGCTCGGTGACAGAGATGGAGAATCATACCTAAATTTTGTTCGATCTAGAGTA
CCAAACCCAATAGGAGGAATTAATGTGGCTTTCACCTTTCTGGCTGGGCTCACTGGGGTT
GTGATAACTCTTGCACTGATCTTAATAATTACATCCTCGACAAAGACTATTCGCAGATCA
TATTTTGAAGTATTCTGGTATACACATCACCTGTTTGTTGTCTTCTTCATTGGTCTAGTC
ATACATGGTGTTGGGCGAATAGTGCGAGGCCAAACAGCTGCCAGTCGAGAGAAGCATAAT
CCAAGAGATTGTGAATATAATTTCACTGAGTGGGGCAACAATACCTCGTGTCCTATCCCC
CAGTTTGCAGGAAATCCACCAGGGACATGGCAATGGGTGATAGCTCCCATGGTCCTGTAT
GTGTTTGAAAGATTGGTCCGATTTTGGAGATCTCAGCAGAAAGTTGTCATCACAAAGGTT
GTAACACACCCATTCAAAACCATTGAATTGCAAATGAAAATGAAGGGGTTCAAAATGGAA
GTTGGACAGTATATCTCTGTCCAGTGTCCAGCAGTGTCCAGATTGGAATGGCATCCTTTT
ACTTTGACTTCTGCCCCAGAAGAAGATTTTTTCAGCATCCACATTAGAATAGTTGGAGAC
TGGACAGAAGGTTTGTTTAAAGCCTGTGGATGTGACAAGACTGAATTTCAAGACGCATGG
AAAATGCCAAAGATTGCAGTTGATGGTCCCTTTGGAACTGCCAGTGAGGATGTTTTTAGC
TATGAGGTTGCAATGCTGGTGGGAGCTGGTATTGGAGTTACTCCCTTTGCTTCTGTTCTG
AAGTCAGTCTGGTATAAATATGTCAATGACGCGTCAACCCTCAGGCTGAAAAAGATCTAC
TTCTACTGGCTGTGCAGAGATACACAAGCTTTCGAGTGGTTTGCGGATTTACTTCAGTCT
CTGGAAACCCAAATGCAAGAGAGAGATAATGCTAATTTCCTTGTCTATAACATCTACCTC
ACTGGCTGGGATGAGTCACAGGCCACTGCCTTCTCTTTGCACCATGACGAAGAGAAAGAT
GTGATTACAGGTCTTAAGCAAAAAACCTTGTATGGCAGACCAAACTGGGAAAATGAGTTT
AAAACCATTGCTAATCAACACCAAAGTTCCAGAGTCGGTGTTTTCCTCTGCGGCCCGGAA
AGCCTTGCTGAGACTCTGAGCAAGCAGTCTATTGCAAACTCCAGTGTGGACCCCAGAGGA
GTTCATTTCATCTTCAACAAAGAGAACTTCTAA

Protein sequence predicted based on nucleotide sequence

MGNWIVNEGLSIAVIIVWLALNGYLFWNFYLVYDEGEAYFYSRKLYGSALAWARAPAACL
NFNCLLILLPVCRNLLSFLRGSSACCGRSLRRQLDRNLTFHKMVAWMIALHTAIHTGAHL
FNVERLVDARVEANGTIQAALTSLGDRDGESYLNFVRSRVPNPIGGINVAFTFLAGLTGV
VITLALILIITSSTKTIRRSYFEVFWYTHHLFVVFFIGLVIHGVGRIVRGQTAASREKHN
PRDCEYNFTEWGNNTSCPIPQFAGNPPGTWQWVIAPMVLYVFERLVRFWRSQQKVVITKV
VTHPFKTIELQMKMKGFKMEVGQYISVQCPAVSRLEWHPFTLTSAPEEDFFSIHIRIVGD
WTEGLFKACGCDKTEFQDAWKMPKIAVDGPFGTASEDVFSYEVAMLVGAGIGVTPFASVL
KSVWYKYVNDASTLRLKKIYFYWLCRDTQAFEWFADLLQSLETQMQERDNANFLVYNIYL
TGWDESQATAFSLHHDEEKDVITGLKQKTLYGRPNWENEFKTIANQHQSSRVGVFLCGPE
SLAETLSKQSIANSSVDPRGVHFIFNKENF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable