Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATTTCCGTGTGCGCAATGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCCTCTTTTTGGGGAAATTGT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713221
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7530nts
Open reading frame: 1 to 7530
ATGCCTCTTTTTGGGGAAATTGTTGTGATTAAAAGGAATGGCACCGATGGGACCCATTTT
CCACTTACTGCAACTTCTTGTCTATTTGGAAGGAAATCAGAATGCGATATCCGTATTCAG
CTGCCACATGTCTCCAAAGAGCATTGCAAAGTTGAAGTTAAAGCAAACAAAGAGGTCTTT
GTCATCAACATAAGTGCCGTCAACCCAACGCAGTTAAATGGAATAGCAATTCAGCAACCT
GAGCTTCTCAAACATGGAGATATAATTACTATTATTGATCGTTCTTTTAGGTTTGAACAA
CCACCGACGAACCCTCACAGACAATCCACAGGGGGAAATTCCTTAAGCAGCAAGTTGGTT
GAAGATGAGCCATCATCAATCAAAGGTAAAGCGAGTAAGCTCACTCGGAAATCAGAAGGA
AATGTCTCTAGAACCACCCACACACGCCTCTCTCTCCCAATATCGCCCCATAGACGTTCT
TCAAGAGAGAAGAAAGATTTGTCACCATTTGGTGAACTGTATGAGATGCTTAAAAATAAA
GTTCACTTAAAACAAGAGCATGCAAAAACGCCAGCAAAGCAGATCATGGAGAATGGAGAT
TGTATTTTTACTCCTGTTACAACTAGACGAAGCCTTGGAAACGTTAAGGGAACACGGGAA
TCGAGCCTCAATAACACCTTGCAGTTTGAAAGTCAGGAACTTCATGTTGAATCAGCAACA
CTGTCTGAGAAAACTGCCAGTGCATTGAAAAGTCCATCCAGAGGGCGTCCGTCCACAAAA
CTAAAGCAACAAAGGCTTAGTGGTAAGAGAGATTCTCCTGCCAAATCTGTTTCTGAGCTG
CCTCATGAAGAAATCTCAATCAGCTTTCAAGATAATGGACAAAAACAGCCTAATGACAAG
CTAACTCCACGTCGTAGACGGAGTACTTCCAAAGAACAGACACCTGTAAAATCTGGAGTC
TCTGTTTTACCTCTGTCAAGAAACAGGTCTCCTAGATCTGCATCTCCCAAGGACTTACAC
GTAAACGCTCAAGTCTCAAAACCACCGCAAAAACGAAGAAGTTCTGAGCTTGAATTACCT
GAGCCGACTCCAAAAAGAAAAAGGGTCTCATTTGGAGGGCATCTTAGTCCAGAGTTTTTT
GATAAAAGGCTTCCTCCAAACTCTCCTCTTCAAAGAGGTGCAACACCTTCAAGACGAAGT
TTATCACTTGCTGCAACTCGTGTTATTCGAAAGAGCTTTGGTGGTTTCAAACAAGCTGTG
ATTAAGGAAACTTTTGAACCGGCCAAGCGGTCTCTTGGCAAGGCCTCCCCGGCCAAGCGG
TCTCCTGGCAAGGCATCCCCGGCCAAGCGGTCTCCTGCCAAGGCATCCCCGGCCAAGATG
ACCCCAGCCAAGCGGTCTCCTGCCAAGGCATCCCCGGCCAAGATGACCCCAGCCAAGCGG
TCTCCTGCCAAGGCATCCCCGGCGAAGATTACCCCAGCCAAACGTTCTCCTGCCAAGGCA
TCCCCGGCCAAGATTACCCCAGCCAAACGTTCTCCTGCCAAGGCATCCCCGGCCAAGATT
ACCCCAGCCAAACGTTCTCCTGCCAAGGCATCCCCAGCAAAGATGACCCCAGCCAAGCGG
TCTCCTGCCAAGGCATCCCCGGCCAAGATGACCCTAGCCAAACGGTCTCCTGCTAAGATG
ACCCTAGCCAAGCAGTCTCCTGCCAAGGCATCCCCGGCCAAGATGACCCCAGCCAAGCGG
TCTCCTGCCAAGATGACCCCAGCCAAGCGGTCTCCTGCCAAGATGACCCCAGCCAAGCGG
TCTCCTGCCAAGATGACCCCAGCCAAGCGGTCTCCGGCCAAGATGACCCCAGCCAAGCGG
TCTCCGGCCAAGATGACCCCAGCCAAGCGGTCTCCGGCCAAGATGACCCCAGCCAAGCGG
TCTCCGGCCAAGATGACCCCAGCCAAGCGGTCTCCGGCCAAGATGACCCCAGCCAAGCGG
TCTCCGGCCAAGATGACCCCAGCCAAGCGGTCTCCGGCCAAGATGACCCCAGCCAAGATG
ACCCCAGCCAAGCGGTCTCCGGCCAAGATGACCCCAGCCAAGCGGTCTCCGGCCAAGATG
ACCCCAGCCAAGCGGTCTCCGGCCAAGATGACCCCAGCCAAGCGGTCTCCGGCCAAGATG
ACCCCAGCCAAGCGGTCTCCGGCCAGGGCATCCCCAGCCAAGCGGTCTCCGGCCAGGGCA
TCCCCAGTCAAGCGGTCTCCTGCCAGGGCATCCCCGGCTAAGATGACCCCAGCCAAGCGG
TCTCCTGCCAATGTTAAAGCAGCCAAACGATCTTCTGCAAAGATGACCCCGGCAAAGTTC
TCACCAGCCAAGCGATCCCCGGCCAAGGTTACACCTGCCAAGAAGTCTCCTGCTACTATT
TATTCAAAAGGACGTTTCTCAATTTCACAAACTGATACACCACCTTTAAATTATGTCCAT
GTTCCTAACACTGAGGAAAATGAACTGTCTGCCCAAACACCAAGGAAATCGCGGAAGTCA
ATATCCTTAAAGAAAACACCAGGAAGAAGGAGTAGAAAACTTGAAACTTTTGAAATTCTG
CGTTCCAGGCGAAGAAGTGGGGCAACTGAAGCTAATTTGCTTGTTGCAAAATCATGGGCT
GATGTTGTACGAATTGGTGTTCCAAAAAATCACAAGAAATCTGAAAAGCAAGCTCACAAA
GCAGTACCTACCAAGAGAAAACAAAAACCAAAGACTCCAGCACGTAGATTTATTGACCGC
AGTGGTACAGGACATGCAGATTCTCCTGCCACAATCCTTGTTGGAAGAGCTCATACAAGA
ACGGTCAACCCAACTGGATATGTTCCTAAAATTGTGCAAAACCAAGCTATTAAAATCATT
AATGACCAAAATGAAAGCTTCACAGGTATGAGTGAGCTGTTTAATACACCTGCACAGGTT
AAACCAAGAAAAAGTGGTCGCTCGGGTGGCCTTCAGGTGAACACTCCACAAGCTCTAATT
GTTGATGTGTCTCTGATGCAGACTCCAGAGGAGTTAGGTGAAATGGTTGTTTCTCCAATA
AATGGCAGCCCTTCTACAACACGTAAGAAACAATACAGTCGTGATGCAGTCTCCCGTCTT
CTAAGAAGTCCTGTGTCCCCGGCATCTCCCAAAGGGATCGAGAAGATATCTGAAACTTCC
AAAGACTCAGAACTGATTGAAAAGGAGACTGCAACATTAAATGGAAACCACTCTGTAAAA
GGGGGAGCAAAGCCAGAAAAGAAAAAGAGAGAAACCATGGGTTTGACTGGTGTGAAAAGG
ATTATGAGAACACCAAAACAAAAAGGAAAGCAAGTTATTGATCCTGTGGCATTAAAGAAA
ATGTTGAGAACACCGAAACAAACACAGGATATGGTTGGCATTAAGTGTATTTTGAAGACT
CCTAAACAGAAGGGGATGCCTGTTGAGGATATGGCAGGCATTAAGCGCATTATGAGGACA
CCCAAGGTTAAAGGAAACCCAGTAGAAGATATGACCGGAATCAAACACATTATGAAAACC
CCCAAGGAGAAAGGGCAACCCGTGGAGGACATGGTGGGAGTCAAGCGTATAATGAGAACC
CCCAAGATAAAAGGGCAACCCGTGGAGGACATGGTGGGAATCAAACGTATAATGAGAACA
CCAAAGGAGAAAGGGCAACCAGTGGAGGACATGGAAGGTTTAAGTCAGCTTATGAGCACC
CCAACAGAAAATACTCGCCCAATAGAAGAAATATTTGGAATGAAACAACTAATAAAGACT
CCTCCAAAGACAACACTTGTTGTTAGAGAGCGTGCATCTGGTACACCTCCACATTCAAGC
AAAAAGGCTGCTCTGTTTCAAACAAATCAAACATCCGCACCAGAAGAAGCATTACCAAAA
CATACAACGGAGACAAATAATGGTGATGTTTCCTTACAAATTGACAGTGAAACTGGAAAA
TCTGAAAAGGCCAAAAGAAGAGGTCGACCTTCCAGGGCCAGTCTGAATGTTGCATTAGAT
GGTGGGAAGTCTGTTGAAAACAATAGCACCTCTGCACAAGAAATAAATATGGAAGAAGCA
TCTCCCCTACATGTACTTGCCACTGAAGATACTTCATCCACGGGCGCTGATAAAGCCACT
CCAGCCAAAGGTAGAGGTCGTCCATCCAAGGGCAGTATGAGCAAGTCACCAGCATCTGCT
AATAGAGATAGTGAGAAGAATAGCACATCATCAGCTCTTGAACATCATGTGGAAGAAAGC
AGCAAAGAATCATCTCTACAGGCATCTACCACAGAAGGTGCTTCATCCATAAATACTGAT
AACGCCACTCCAGCCAAGCGAAGAGGTCGTCCATCCAAACGCAGTTTGAGTCATTCACCA
GTTCAAACTATTCAAACATCAGCACCAGAAGAAGCCTTACCAAAACATACAATGGAGACA
AATAATGGTGAGGTTTCGTTACAAATTGACAGTGAAACTGGAAAATCTGAAACAGCCAAA
AGAAGAGGTCGACCTTCCAGGGCCAGTTTGAATGTTGCATTAGATGGTGGGAAGTCTGTT
GAAAACAAAAACACTTCTGCACAAGAAATAAATATGGAAGAAGCATCTCCCCTACATGTA
CTTGCCACTGCAGATACTTCATCCACGGGCGCTGATAAAGCCACTCCAGCCAAAAGTAGA
GGTCGTCCATCCAAGGGCAGTATGAGCAAGTCACCAGCATCTGCTAATAGCGATGGTGGG
AAGTCTGTTGAAAACAATAGCACTTCTGCACAAGAAATAAATATGGAAGAAGCATCTCCT
CTACATGTACTTGCCACTGAAGATACTTCATCCCCGGGCACTGATAAAGCCACTCCACCC
AAAGGTAGAGGTCGTCCATCCAAGGGCAGTATGAGCAAGTCACCAGCATCTGGTAATGGA
GATAGTGAGAAGTCTGAAAAGAATAGCACATCATCAGCTCTTGAAAATCATGTGGAAGAA
AGCAGCAAAGAAGCTTCTCTACAGGCATCTACCACAGAAGGTGCTTCATCCACAGATGCT
GATAAAACCACTCCAGCAAAGAGAAGAGGTCGTCCATCCAAACACAGTTTGAGTCATTCA
CCAGATGGTGGGAAGTCTGTTGAAAACAATAGCACCTCTGCACAAGAAATAAATATGGAA
GAAGGAGCTCCTCTACATGTACTTGCCACTGAAGATTCTTCATCCACGGGCGCTGATAAA
GCCACTCCAGCCAAAAGAAGAGGTCGTCCATCCAAGGGCAGTATGAGCAAGTCACCAGCA
TCTGCTAATAGAGATAGTGAGAAGTCGGAAAAGAATAGCACATCAGCTCTTGAAAATCAT
GTGGAAGAAAGCAGCAAAGAAGCTTCTCTACAGGCATCTACCACTGAAGGTGCATCATCC
ACAGATGCTGATAAAACCACTCCAGCAAAGAGAAGAGGTCGTCCATCCAAAAGCAGTTTG
AGTCATTCACCAGTGAAACCTGCTGTAGATGTTCCAGAAGACCTAAGTACCAGCAATGAG
GATTCTAATATTTCTGAACTATCAAGTGATATCAGAACAGACCAACAGAAAAATGTTTTA
CCTAATGGAGCAAGAAAAGCAACTAAGAAATCTCAGAGAAAGAATACAGTTACAAAGGAA
TCTGTCAAAATAGCAGCAGTGCTGCAATCGGAACCTTTGGAAAACTTGGTTGTTGAAAAT
GATGAAAAGTCTGCTCCCCAAAGGCGACGAGGAAGACCTCAAAAGGGTGGTGCACTTTCA
TCTGCCACTGAAGTTTCAGATGGAGTGTTAGCAGAAGAACTAACAACAAACAGACGAACT
GGCAGAGGCCGAAAGATAGCAGAAGCGGCAAAACTGCAAGAGGGTTTGGAAGTCTGTACA
AGTGCTGCAGAATCTCAAGTGGAAGTAAAAAATGAGGAACTCTCGTCAACTAAGAGTTTG
TCTAAATCTAGCAGTCCAATAAAAGCTGTACCTGAAGACTCTACAACAACTAAGAAGCTA
CGTGGAAGGCAGCCTAAAGTGGGCAGAGAACGGAAAGCTGTTGCAGAGAAACTGCTTACA
GAACTGCCAGGAGATTCAGCTTTAGTTAACAATGTAACTCCATCTGTGAGAAGAGGTAGA
CGAGCAAGAGGCACTGCAAACTCTGTGACTGAAACAGTCATAATTGAAACAAATTTACCT
GACTTGGCAGACACAGTAGTTTTGCATGAAGAGCAAGAAAGTTCCAGTCATACCTCTCCA
GAAGCAAAAACCCAAAGAGGAAATCGAAAAAATCTCAAAAGTGCTGTTGATTCTGTAAAG
AACTCTGCTCAAACAGATGTAACAATAGAATTAAAAGAAAGAGCTGGCAAAAGACCTACA
CGTGGTGCAAGAGGCACTCAGCAGGTTGAGGAGCTTAAAGAACTAAAAGGAAAAAAAGCA
GATTACCATATTCCCAAGAATGCTGAACCAGTTCTTTTAGAGGAGACTCTAGCTAAAGAG
AAGGCATCTAAGGCAAAATCTGTGCAATGGCACCCTCTGCTGGCTGCTCAGGAAACTGCT
GAAAAGCATACGGAGGAGACTGTTAAGAATCTTGCAGATGAAGAAGCACTGGTTAGTGCA
ATTAAATCAAGAGGAAGAAGGAGGCAAGAAGTAAAAGAAACAGTAGTTCCAGCAAAACGA
TCACGTTCAAGAAAGGAGGAAAAAAGTGTTGATACATTAAATTCAAGTAGTGTTGCAGTT
GATGAGAAATCTCTTGCTTTGGTTGCCAGTGACAAAAATGAAATGTCTAAGACTAAGAGA
GGGCGGAGGCAAGCAACCATTCAGTTAAAGAAAATTACTGATCCAGATCTTCCAGTGGCA
GAAGTGAAAAATAATTCAGATGTTTCCTCTAAACAAGATCTTGAACCTGAAATTGTAAGT
GACAAGCCTTTAAACAGAAGAAACACAGGTAAAAGAAATCTTAAGTCTTCATCTGACTCT
GATTCTGGCAGTAATGCGATTGAACCAGCATTGGAGACAATGACACAAAAGCGTGGACGA
AGAGGAGCAGCAGCCAAAATTTCTGACTCTGAGAATACAGAAGTTAAAACTGTTGAGTCT
GCTGAAAACCTCCCTTCAAGTAAAAGAAGGGGCAGAGCTCCAAATAAAAAAAACATGCCT
GAAATTAGCAACGTTGCTGCTGAACCTGCTGAAAGTCCAGAAAATGTGGCTGAAATTGAA
AATACTGAAAAAATAGAAAGCCCCCCTAAATCAAGAAGAGTCTTGAAAAGAAAGGCAGCC
ACCACTGATGTTTCAGAAGGCAACACATCAGTAAGTGAAAAGGAAGCCCCTGCTCCTGTT
AGGGGAAGCACAAGAGGAGCTTCAAGGCAAAAATCTGCAGCAAAAGAGAAACCTGTGGTT
GTTGAAGAATCACCAGCAAAGAGAAAGAAAGCAGTGCCATCTTCCAGTCCAACCCAGAAA
AGAGGAAAAACCAAAGCCACTGCAGTGAAACGGCAAAATGAGAAGCCCGTCGAAGTTGTA
AAGACGAGACCAACATTGCGCACACGGAAATAG
Protein sequence predicted based on nucleotide sequence
MPLFGEIVVIKRNGTDGTHFPLTATSCLFGRKSECDIRIQLPHVSKEHCKVEVKANKEVF VINISAVNPTQLNGIAIQQPELLKHGDIITIIDRSFRFEQPPTNPHRQSTGGNSLSSKLV EDEPSSIKGKASKLTRKSEGNVSRTTHTRLSLPISPHRRSSREKKDLSPFGELYEMLKNK VHLKQEHAKTPAKQIMENGDCIFTPVTTRRSLGNVKGTRESSLNNTLQFESQELHVESAT LSEKTASALKSPSRGRPSTKLKQQRLSGKRDSPAKSVSELPHEEISISFQDNGQKQPNDK LTPRRRRSTSKEQTPVKSGVSVLPLSRNRSPRSASPKDLHVNAQVSKPPQKRRSSELELP EPTPKRKRVSFGGHLSPEFFDKRLPPNSPLQRGATPSRRSLSLAATRVIRKSFGGFKQAV IKETFEPAKRSLGKASPAKRSPGKASPAKRSPAKASPAKMTPAKRSPAKASPAKMTPAKR SPAKASPAKITPAKRSPAKASPAKITPAKRSPAKASPAKITPAKRSPAKASPAKMTPAKR SPAKASPAKMTLAKRSPAKMTLAKQSPAKASPAKMTPAKRSPAKMTPAKRSPAKMTPAKR SPAKMTPAKRSPAKMTPAKRSPAKMTPAKRSPAKMTPAKRSPAKMTPAKRSPAKMTPAKR SPAKMTPAKRSPAKMTPAKMTPAKRSPAKMTPAKRSPAKMTPAKRSPAKMTPAKRSPAKM TPAKRSPARASPAKRSPARASPVKRSPARASPAKMTPAKRSPANVKAAKRSSAKMTPAKF SPAKRSPAKVTPAKKSPATIYSKGRFSISQTDTPPLNYVHVPNTEENELSAQTPRKSRKS ISLKKTPGRRSRKLETFEILRSRRRSGATEANLLVAKSWADVVRIGVPKNHKKSEKQAHK AVPTKRKQKPKTPARRFIDRSGTGHADSPATILVGRAHTRTVNPTGYVPKIVQNQAIKII NDQNESFTGMSELFNTPAQVKPRKSGRSGGLQVNTPQALIVDVSLMQTPEELGEMVVSPI NGSPSTTRKKQYSRDAVSRLLRSPVSPASPKGIEKISETSKDSELIEKETATLNGNHSVK GGAKPEKKKRETMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQTQDMVGIKCILKT PKQKGMPVEDMAGIKRIMRTPKVKGNPVEDMTGIKHIMKTPKEKGQPVEDMVGVKRIMRT PKIKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQLMSTPTENTRPIEEIFGMKQLIKT PPKTTLVVRERASGTPPHSSKKAALFQTNQTSAPEEALPKHTTETNNGDVSLQIDSETGK SEKAKRRGRPSRASLNVALDGGKSVENNSTSAQEINMEEASPLHVLATEDTSSTGADKAT PAKGRGRPSKGSMSKSPASANRDSEKNSTSSALEHHVEESSKESSLQASTTEGASSINTD NATPAKRRGRPSKRSLSHSPVQTIQTSAPEEALPKHTMETNNGEVSLQIDSETGKSETAK RRGRPSRASLNVALDGGKSVENKNTSAQEINMEEASPLHVLATADTSSTGADKATPAKSR GRPSKGSMSKSPASANSDGGKSVENNSTSAQEINMEEASPLHVLATEDTSSPGTDKATPP KGRGRPSKGSMSKSPASGNGDSEKSEKNSTSSALENHVEESSKEASLQASTTEGASSTDA DKTTPAKRRGRPSKHSLSHSPDGGKSVENNSTSAQEINMEEGAPLHVLATEDSSSTGADK ATPAKRRGRPSKGSMSKSPASANRDSEKSEKNSTSALENHVEESSKEASLQASTTEGASS TDADKTTPAKRRGRPSKSSLSHSPVKPAVDVPEDLSTSNEDSNISELSSDIRTDQQKNVL PNGARKATKKSQRKNTVTKESVKIAAVLQSEPLENLVVENDEKSAPQRRRGRPQKGGALS SATEVSDGVLAEELTTNRRTGRGRKIAEAAKLQEGLEVCTSAAESQVEVKNEELSSTKSL SKSSSPIKAVPEDSTTTKKLRGRQPKVGRERKAVAEKLLTELPGDSALVNNVTPSVRRGR RARGTANSVTETVIIETNLPDLADTVVLHEEQESSSHTSPEAKTQRGNRKNLKSAVDSVK NSAQTDVTIELKERAGKRPTRGARGTQQVEELKELKGKKADYHIPKNAEPVLLEETLAKE KASKAKSVQWHPLLAAQETAEKHTEETVKNLADEEALVSAIKSRGRRRQEVKETVVPAKR SRSRKEEKSVDTLNSSSVAVDEKSLALVASDKNEMSKTKRGRRQATIQLKKITDPDLPVA EVKNNSDVSSKQDLEPEIVSDKPLNRRNTGKRNLKSSSDSDSGSNAIEPALETMTQKRGR RGAAAKISDSENTEVKTVESAENLPSSKRRGRAPNKKNMPEISNVAAEPAESPENVAEIE NTEKIESPPKSRRVLKRKAATTDVSEGNTSVSEKEAPAPVRGSTRGASRQKSAAKEKPVV VEESPAKRKKAVPSSSPTQKRGKTKATAVKRQNEKPVEVVKTRPTLRTRK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
