gmip (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATCCTTCTTCACCCTCGGCT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCGTCAGGTGGAGG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713360
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2580nts
Open reading frame: 1 to 2580

ATGGCGTCAGGTGGAGGAGGAGTTTCAGGCACCAGCACACCGAGTGGTTCCTCGGAGGGT
CGGAAGAGATTCAGTGAAATATTCCGAGATTTTGATGCGCTGGAGATAAGCCTCGGAAAT
GAGACGGTGGAAATGTTCTTGAATGATTCAGAGCATTTGGAACTCCACCCAGACGAAGAA
GAGGAGTCACTGATCAGCACACCGTTGGAGCTATCACGGGCATCAGTTGATCAAGGAGTT
GAGGAGACTGATGATATCCTGCTGCACAGTGAAGGTGGAGTGGAGTCAGCACTCAACTAT
GCAAAGCGTTGGTGCAAATATGTGAGGGAGGTGTTGGGTTATATGGAGAAAAGACTCAAC
TATGAACTTGAATTTGCTAAGAACATCACTAAACTGGCCGAGTCCACCAGATTCATTTTC
ACCCAACAGGCATCCATGCCTTTACAATGTGTCTACCTCTTGCTCATGGACCATGAGACC
CAGACAGCGAACGCTGCTCATGATACGGTAGCCCAGTTACAGTTGAAGAAATATTGCCAG
CCGCTGTCTGCAAAGAAGACAGAAATTGAAAAATGGAGGAAAGAATTTCGGGAGCAGTGG
CAGAGAGAGCAGAAACGAATGATTGACTCGTGTGCGGCACTGCGCAAGTCTCGGCAACTA
TACATTCAACGCTGTGATGACCTAGAAAAGGCAAAGCAACTAAGTGCAAAGATAGAGGAA
GAACAGGGTCACACAGGAAGTGCAAACAAACTTGTGGAAAAGAGAAGGAAGTCCCGGGAG
GAGGCCCAGTCCAAGGCCACAGAAGCGGAGATGGCATATCGTTACTGTGTACAGGAGGCC
AACGCTCGCTCTCAGCAGCAAGAAAAGGTCAGAGAGAGAATTGTATCGCACATTAGGAAG
CTGATTATCCAGGGGGATCAAGTGCTGAAGGAGGTGACCATGAACCTCTTGCGTATGAAG
CAATCCCAGTGTGAGGTTATCCCTTGTGGTTATGGGGACCTGCTGACTACGTGCGACCCC
TATGAGTCTGGGACTCGATACCTGCAGTTCATATTGACTCTGCCACGGAAGCACACAGAT
CCTGAACGGTTCACCTTCGAGGAATATATCCCAACAGGACAAAGGTCACCATCTGTTGGT
AGAAGGAAGAATGCAGTGCAGCTTGTCAGAGTCTCCTCGTCTTTATCTGACCTCAATGTA
AAGAACGAGGATGGAACAGTAAAGATGTATGCAGGGGAAAGGGGATCATGGGGCAGCAAA
TTTCCCAGCAGTGATTCAGAGAGCATGGGAGCTACCAGCGAATCCAGATCCCAAGAGTCT
ACAAGCGACAGCCCAGGTGACTTTGCTAGAAGAGTCCCTAAAGCTTCCTCCACAGGTACT
ATGTCTTCTGATGATCTGGATGAAAGAGATTCCTTCAACACAGAGGATGTTGATTCAACA
GATGCCAATTCTGAGAATGGTTTGCCACTGCAGAGCATGGAAAGGCTGGGGGTTCTGTCT
CTGGCTGCTCAAAGTCATCGTCTTAAGAGGACAAGAGTTCCCAGCAAGTGCAGGGAGTGC
GAGAACTTTATGGTTTCTGGTGTGGAGTGTGAGGAGTGCTTACTGACCTGTCACAGAAAG
TGCTTGGAAAACCTGCTTATAAAGTGTGGTCATCGGAAGCTTCCTCCTAAAGTTCCCCTG
TTTGGAGTAGACTTCAGCCAATTTCCTCGGTACTTTCCAGAAGAGGTTCCTTTCATCATT
GTTAGATGTACTGCAGAAATTGAACAACGGGCACTGGGGCAACAGGGTTTGTATCGTATC
AGTGGAGCCAAAGCTCGGGTGGAGAAGCTCCTGCAGGCATTTGAGAATGGGAGAGAACTG
GTGGACCTTTCAGGCCATTCACCGCATGATATAACAAGCTCACTCAAGCATTTTCTAAAG
CAGCTCCCAGACTCAGTTGTCCCTTATCAACTTTATGAGCAATTTATGGCTTTTTCAAGA
GACTACCTGGAAGACACAAAGGAGAATGAAACTGGCCACAGTGCTATTCATCAAATGAAG
GAACTTCTGTGCAGGATGCCTCACAGTCACTACAACACACTTCGTCATCTCACAGCCCAT
CTCTACAGAGTGTCAGAGAGGTTTGAGGATAACAAGATGAATCCTAATAACCTGGGGATA
ATCTTTGGACCAACTCTAATTCGTCCATTGCCTGGTCAGGACATATCAGTGAACTGTCTG
ATTGACACTGGGTACCAGTCACAGGCAGTGGAATTCCTTATAAACAACTATGAGAAGATC
TTTGGGATGGATGACTTACCCTCTGCCAGACCCCAGAATTCAGAAGAGGAGCCAGCAGAT
GTGGAAGGAGCAGAGGATTCAAGAAGACCCTTTGATGTTAAGGGTGTAACAGAGACTTCA
TCAGACAAGGGTCTATCAGATGCTGACAGACTCCAAGAAGAGGTGGAAGAAGACCAAGAG
GTGGAAAATGCCACATATTCCAGGAATCACTTTAGTCGCTTGCCGATAAGGCTTGTGCGT
ATGGAACAGTCAAAGACTGAAGGAGCAGAGACTGATGGAGAAGCCGAGGGTGAAGAAGGA
TAA

Protein sequence predicted based on nucleotide sequence

MASGGGGVSGTSTPSGSSEGRKRFSEIFRDFDALEISLGNETVEMFLNDSEHLELHPDEE
EESLISTPLELSRASVDQGVEETDDILLHSEGGVESALNYAKRWCKYVREVLGYMEKRLN
YELEFAKNITKLAESTRFIFTQQASMPLQCVYLLLMDHETQTANAAHDTVAQLQLKKYCQ
PLSAKKTEIEKWRKEFREQWQREQKRMIDSCAALRKSRQLYIQRCDDLEKAKQLSAKIEE
EQGHTGSANKLVEKRRKSREEAQSKATEAEMAYRYCVQEANARSQQQEKVRERIVSHIRK
LIIQGDQVLKEVTMNLLRMKQSQCEVIPCGYGDLLTTCDPYESGTRYLQFILTLPRKHTD
PERFTFEEYIPTGQRSPSVGRRKNAVQLVRVSSSLSDLNVKNEDGTVKMYAGERGSWGSK
FPSSDSESMGATSESRSQESTSDSPGDFARRVPKASSTGTMSSDDLDERDSFNTEDVDST
DANSENGLPLQSMERLGVLSLAAQSHRLKRTRVPSKCRECENFMVSGVECEECLLTCHRK
CLENLLIKCGHRKLPPKVPLFGVDFSQFPRYFPEEVPFIIVRCTAEIEQRALGQQGLYRI
SGAKARVEKLLQAFENGRELVDLSGHSPHDITSSLKHFLKQLPDSVVPYQLYEQFMAFSR
DYLEDTKENETGHSAIHQMKELLCRMPHSHYNTLRHLTAHLYRVSERFEDNKMNPNNLGI
IFGPTLIRPLPGQDISVNCLIDTGYQSQAVEFLINNYEKIFGMDDLPSARPQNSEEEPAD
VEGAEDSRRPFDVKGVTETSSDKGLSDADRLQEEVEEDQEVENATYSRNHFSRLPIRLVR
MEQSKTEGAETDGEAEGEEG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable