hsf2 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATCCAGCAGTGGCATATCATT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAAGCAGAACTCGAATGTTCC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713459
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1545nts
Open reading frame: 1 to 1545

ATGAAGCAGAACTCGAATGTTCCGGCATTTCTCAGCAAGCTTTGGACTTTAGTGGAGGAT
TCTGACACAAACGAGTTCATTACTTGGAGTCAGAATGGACAGAGCTTTTTGGTTCTTGAC
GAGCAGCGGTTTGCTAAAGAGATTCTACCAAAGTATTTCAAGCACAACAACATGGCTAGC
TTTGTAAGACAATTAAACATGTATGGTTTCCGTAAAGTGGTACATATAGACTCTGGCATT
GTTAAGCAAGAGCGGGATGGACCAGTGGAATTTCAACACCCATTCTTTGTCCAAGGGCAG
GATGAATTATTGGAAAATATAAAGAGGAAGGTGTCCTCAACAAGACCAGAAGAAGGCAAA
GTGTGCCAGGAAGATATATCGACAATTTTAAGCAATGCTGCAAAGGTCCAAGTCCAACAA
GAAACTATTGATTTGCGATTGTTTACATTAAAAAGGGACAACGAAGCTTTGTGGAGAGAA
ATATCTGACTTGCGTAACAAACATGTCCAGCAGCAACAAGTTATTCGAAAGATTGTGCAG
TTTATTGTGACTCTGGTGCAGAAAAACAGATTAGTCAGTCTAAAGAGAAAACAGCCATTA
CTTTTGAACACAAATAACTCTCCAAAATCCACGCGCCTACAGACGATGGTTAAGGAGACA
GTGGAAGATAATCATCATGTTTCCCTTAGGAGTGAAGGCTTAAAAAGAGGGGAGCTTCCA
GAAGACATTGTCATATATGACATAACTGATAACATGGAATTTGAGGAGGAGGAGGTAAAG
CAACAGGAAGCAGGAGCTCATGCTGTTTGTGAAAGTTCTGGTGAAAGCACCTCGAATGCG
GTGGCTGAGAGGCTTTCACCAGAAATTGTAATAGTGGAAGATGATAGTGATTATGAAGAC
GCTCCAGTTGTGCAAGGAAAAAAAAATGCTGAGACTGGTCTTCGTAGCCCTGCTGGTGAG
AACACGTCTGTTCCTCTGATGTCAAGTGCCCTACAGCTCAACAGCCAGTCTTCTTTTAAT
CTAGAGGATCCAGTTACAATGATGGATTCCATTCTTAATGAAAGTGGCGTTATTTCTCAA
AACCTCAACCTTCTTGGAAAAGTTGAGCTGATGGATTACTTAGACAGCATTGATTGCACG
TTGGATGATTTCAAAGCTTTACTGTCTGGGCGTCAATTCAGCATAGACCGAGATCTGCTG
GTAGATCTGGAAAGTAAAGGCATTGAATCTCTGAAGAGCTGTGGTGTTGTCCCAGGACCT
GCAAATTCCAGTGGGGATAACCATAAATCTGATCATCAGCTTGTGCAATACACTTCATTT
CCACTTCTGGCTCTTTTTGACGGGGACAGTGGAAATTTAACAACAGACACACCACCAGAC
AAACCACTTGAAGACCAGCTTCTGGAATCCAACTTGGACTCTCCACCACAAAGCAAATTA
ATGCGTCTTGAACCACTGTCGGAAGCAGAGGCCAGTGAGGCTACCCTTTTCTATTTATGT
GAACTGGCACCAGCCCCTCTGGACAATGATATGCCACTGCTGGATTAA

Protein sequence predicted based on nucleotide sequence

MKQNSNVPAFLSKLWTLVEDSDTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMAS
FVRQLNMYGFRKVVHIDSGIVKQERDGPVEFQHPFFVQGQDELLENIKRKVSSTRPEEGK
VCQEDISTILSNAAKVQVQQETIDLRLFTLKRDNEALWREISDLRNKHVQQQQVIRKIVQ
FIVTLVQKNRLVSLKRKQPLLLNTNNSPKSTRLQTMVKETVEDNHHVSLRSEGLKRGELP
EDIVIYDITDNMEFEEEEVKQQEAGAHAVCESSGESTSNAVAERLSPEIVIVEDDSDYED
APVVQGKKNAETGLRSPAGENTSVPLMSSALQLNSQSSFNLEDPVTMMDSILNESGVISQ
NLNLLGKVELMDYLDSIDCTLDDFKALLSGRQFSIDRDLLVDLESKGIESLKSCGVVPGP
ANSSGDNHKSDHQLVQYTSFPLLALFDGDSGNLTTDTPPDKPLEDQLLESNLDSPPQSKL
MRLEPLSEAEASEATLFYLCELAPAPLDNDMPLLD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable