cdk1-b (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  cdk1-b
Gene Name:  cyclin-dependent kinase 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATTTCTAATCTGATTGGCGGG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGACGAGTACACTAAAATAGAG, XGC Collection: IRBG
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713648
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 906nts
Open reading frame: 1 to 906

ATGGACGAGTACACTAAAATAGAGAAGATTGGAGAGGGCACATATGGGGTCGTGTACAAG
GGTCGTCACAAAGCAACCGGCCAGGTCGTCGCAATGAAGAAAATTAGATTGGAAAATGAA
GAGGAAGGTGTCCCAAGTACAGCAATCCGAGAAATATCACTACTTAAAGAGCTTCAGCAC
CCCAACATAGTCTGCCTCCTAGATGTCCTCATGCAAGACTCAAGGTTGTATCTTATCTTT
GAGTTTCTCTCCATGGATCTAAAGAAGTATTTAGACTCGATACCCAGCGGCCAGTATATC
GATACAATGCTCGTTAAGAGTTACCTGTACCAGATCCTACAAGGGATTGTATTCTGTCAC
TCCAGACGAGTTCTACACAGAGACCTGAAACCTCAAAACCTGCTCATAGACAATAAAGGA
GTGATAAAGTTGGCAGATTTTGGCCTTGCCAGAGCTTTTGGAATTCCGGTTCGGGTTTAC
ACACACGAGGTCGTGACGTTATGGTACAGAGCCTCGGAAGTGCTGTTGGGGTCAGTCCGA
TATTCAACGCCAGTTGACGTCTGGAGCGTAGGAACTATTTTCGCCGAGATCGCCACAAAG
AAACCCCTCTTCCACGGTGACTCTGAAATTGACCAGCTCTTCAGGATATTCAGATCTTTA
GGGACACCCAACAATGAAGTGTGGCCAGAAGTAGAGTCTTTACAAGATTACAAGAACACA
TTCCCCAAATGGAAAGGGGGAAGCCTGTCGTCGAATGTGAAAAATATTGATGAGGATGGG
CTGGACCTGCTGTCTAAAATGCTAGTCTATGATCCCGCCAAGAGGATTTCCGCACGAAAA
GCTATGTTGCACCCCTACTTCGATGACTTGGATAAGTCCAGCCTTCCCGCCAATCAGATT
AGAAATTAA

Protein sequence predicted based on nucleotide sequence

MDEYTKIEKIGEGTYGVVYKGRHKATGQVVAMKKIRLENEEEGVPSTAIREISLLKELQH
PNIVCLLDVLMQDSRLYLIFEFLSMDLKKYLDSIPSGQYIDTMLVKSYLYQILQGIVFCH
SRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGIPVRVYTHEVVTLWYRASEVLLGSVR
YSTPVDVWSVGTIFAEIATKKPLFHGDSEIDQLFRIFRSLGTPNNEVWPEVESLQDYKNT
FPKWKGGSLSSNVKNIDEDGLDLLSKMLVYDPAKRISARKAMLHPYFDDLDKSSLPANQI
RN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable