| Gene: |
Gene Symbol:
t-b Gene Name: T, brachyury homolog Sequence |
|---|---|
| Original Clone ID: | None |
| Keyword: | None |
| Species: | Xenopus laevis |
| Type: | cDNA |
| Vector Name: | pDONR223 Format: FUSION |
| Source: | Xenopus Gene Collection |
| Description: | None |
| Comments: | The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGATTGATGGTGGTGCAACT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGTGCGACCGAGAG, XGC Collection: IRBH |
| Mutation/ Discrepancy : |
No / No |
| Publications: | None |
| Authors: |
Center for Cancer Systems Biology Cincinnati Children's Hospital Medical Center MRC National Institute for Medical Research University of Virginia School of Medicine Aaron Zorn David E. Hill Michael J. Gilchrist Todd Stukenberg Xenopus Gene Collection |
|
XlCD00713743 Price: Login for Pricing AVAILABLE No restriction Special MTA: None |
|
Insert sequence: 1296nts
Open reading frame : 1 to 1296 ATGAGTGCGACCGAGAGCTGCGCCAAGAACGTGCAGTACCGGGTAGATCATCTGCTGAGC
GCTGTGGAGAATGAGCTCCAGGCTGGCAGCGAAAAGGGGGACCCCACCGAGAAGGAGCTG
AAGGTGAGTCTGCAGGAGCGGGACCTGTGGACGAGGTTTAAGGAGCTCACCAATGAGATG
ATCGTCACTAAGAATGGAAGGAGAATGTTTCCAGTTCTGAAGGTGAGCGTGTCGGGCCTG
GATCCCAATGCCATGTACACGGTTCTTCTGGACTTTGTGGCAGCTGACAACCTCCGCTGG
AAGTATGTGAATGGAGAATGGGTTCCAGGTGGCAAACCTGAGCCCCAGGCCCCTAGTTGT
GTTTACATCCACCCAGATTCCCCCAATTTTGGAGCCCACTGGATGAAAGATCCTGTGTCT
TTTAGCAAAGTCAAACTTACAAACAAAATGAATGGTGGAGGCCAGATTATGTTAAACTCT
TTGCACAAGTATGAACCTCGAATCCACATTGTGAGAGTTGGCGGCACCCAGAGAATGATC
ACCAGCCATTCATTCCCTGAGACACAGTTCATAGCAGTGACCGCATACCAGAATGAAGAG
ATTACAGCACTTAAAATCAAGCACAACCCATTTGCCAAAGCATTTCTTGATGCAAAAGAA
AGAAATGATTATAAAGACATCTTGGATGAGGGAATTAATAGTCAGCACTCAAATTTCTCT
CAGTTAGGTACTTGGCTCATTCCTAATGGTGGATCATTATGTACGCCCAATCCACACACT
CAGTTCGGGGCACCTCTCTCTCTATCATCTCCTCATGGCTGTGAGCGATACTCATCTCTG
AGGAACCACCGCTCCGCTCCTTACCCCAGTCCATACACTCACAGAAACAATTCTCCAAAC
AATTTAACAGATAATTCCTCAGCATGTCTTTCAATGCTCCAGTCCCATGGCAACTGGTCC
ACACTTCAAATGCCAGCACACACAGGGATGTTGCCAATGAGTCACAGCACAGGAACACCA
CCTACCTCAAGTCAGTACACTTCTTTGTGGTCTGTAAGTAACAGTACCATCACACCAGTG
TCTCAGTCTGGAGGAATAAGTAATGGAACAAGTCCCCAGTACCTACGTGGCTCTACACCT
CATTACTCATCTCTTTCACATGCTGTGCCCTCACCAACCACAGGATCCCCATTGTATGAA
CATGGAGCACAAACAGAAATAGCAGAAAACCAGTATGATGTCACTGCACATTCCAGGCTT
TCATCAGCATGGACACCAGTTGCACCACCATCAATCTAA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: End read sequencing |
|
Mutations: None |
Discrepancy: None |
| 5' Linker Sequence: None | |
| 3' Linker Sequence: None | |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
spectinomycin Bacterial Selection Condition: 100 ug/mL spectinomycin Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin. Comments: None |
| Protein Expression Results: | None |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pDONR223 |
pDONR223 in advanced viewed |
| Synonyms: | '' | |
| Sequencing Primer: | Forward:
M13F
Reverse: M13R |
|
| Description: | Recombinational donor/master vector; spectinomycin resistance; recombinational cloning. | |
| Comments: | pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors. | |
| Size (bp): | 5005 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | Gateway, donor (entry), recombinational cloning | |
| Author Name: |
Invitrogen |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | pBR322 origin | pBR322 bacterial origin | 4339 | 4958 |
| death cassette | ccdB | ccdB negative selection gene (death cassette), LOST in recombined (with insert) form | 1200 | 1409 |
| primer | M13 forward | M13 forward sequencing primer | 536 | 553 |
| primer | M13 reverse | M13 reverse sequencing primer | 3020 | 3040 |
| recombination site | attP1 | attP recombination site 1 present in empty vector with death cassette, replaced by attL1 in recombined form with gene of interest or other insert | 569 | 652 |
| recombination site | attP2 | attP recombination site 2 present in empty vector with death cassette, replaced by attL2 in recombined form with gene of interest or other insert | 2896 | 2983 |
| selectable marker | SpectR | spectinomycin resistance gene | 3471 | 4262 |
| trxn termination sequence | rrnB | rrnB T1 and T2 terminator | 268 | 470 |
