t-b (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  t-b
Gene Name:  T, brachyury homolog
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGATTGATGGTGGTGCAACT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGTGCGACCGAGAG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713743
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1296nts
Open reading frame: 1 to 1296

ATGAGTGCGACCGAGAGCTGCGCCAAGAACGTGCAGTACCGGGTAGATCATCTGCTGAGC
GCTGTGGAGAATGAGCTCCAGGCTGGCAGCGAAAAGGGGGACCCCACCGAGAAGGAGCTG
AAGGTGAGTCTGCAGGAGCGGGACCTGTGGACGAGGTTTAAGGAGCTCACCAATGAGATG
ATCGTCACTAAGAATGGAAGGAGAATGTTTCCAGTTCTGAAGGTGAGCGTGTCGGGCCTG
GATCCCAATGCCATGTACACGGTTCTTCTGGACTTTGTGGCAGCTGACAACCTCCGCTGG
AAGTATGTGAATGGAGAATGGGTTCCAGGTGGCAAACCTGAGCCCCAGGCCCCTAGTTGT
GTTTACATCCACCCAGATTCCCCCAATTTTGGAGCCCACTGGATGAAAGATCCTGTGTCT
TTTAGCAAAGTCAAACTTACAAACAAAATGAATGGTGGAGGCCAGATTATGTTAAACTCT
TTGCACAAGTATGAACCTCGAATCCACATTGTGAGAGTTGGCGGCACCCAGAGAATGATC
ACCAGCCATTCATTCCCTGAGACACAGTTCATAGCAGTGACCGCATACCAGAATGAAGAG
ATTACAGCACTTAAAATCAAGCACAACCCATTTGCCAAAGCATTTCTTGATGCAAAAGAA
AGAAATGATTATAAAGACATCTTGGATGAGGGAATTAATAGTCAGCACTCAAATTTCTCT
CAGTTAGGTACTTGGCTCATTCCTAATGGTGGATCATTATGTACGCCCAATCCACACACT
CAGTTCGGGGCACCTCTCTCTCTATCATCTCCTCATGGCTGTGAGCGATACTCATCTCTG
AGGAACCACCGCTCCGCTCCTTACCCCAGTCCATACACTCACAGAAACAATTCTCCAAAC
AATTTAACAGATAATTCCTCAGCATGTCTTTCAATGCTCCAGTCCCATGGCAACTGGTCC
ACACTTCAAATGCCAGCACACACAGGGATGTTGCCAATGAGTCACAGCACAGGAACACCA
CCTACCTCAAGTCAGTACACTTCTTTGTGGTCTGTAAGTAACAGTACCATCACACCAGTG
TCTCAGTCTGGAGGAATAAGTAATGGAACAAGTCCCCAGTACCTACGTGGCTCTACACCT
CATTACTCATCTCTTTCACATGCTGTGCCCTCACCAACCACAGGATCCCCATTGTATGAA
CATGGAGCACAAACAGAAATAGCAGAAAACCAGTATGATGTCACTGCACATTCCAGGCTT
TCATCAGCATGGACACCAGTTGCACCACCATCAATCTAA

Protein sequence predicted based on nucleotide sequence

MSATESCAKNVQYRVDHLLSAVENELQAGSEKGDPTEKELKVSLQERDLWTRFKELTNEM
IVTKNGRRMFPVLKVSVSGLDPNAMYTVLLDFVAADNLRWKYVNGEWVPGGKPEPQAPSC
VYIHPDSPNFGAHWMKDPVSFSKVKLTNKMNGGGQIMLNSLHKYEPRIHIVRVGGTQRMI
TSHSFPETQFIAVTAYQNEEITALKIKHNPFAKAFLDAKERNDYKDILDEGINSQHSNFS
QLGTWLIPNGGSLCTPNPHTQFGAPLSLSSPHGCERYSSLRNHRSAPYPSPYTHRNNSPN
NLTDNSSACLSMLQSHGNWSTLQMPAHTGMLPMSHSTGTPPTSSQYTSLWSVSNSTITPV
SQSGGISNGTSPQYLRGSTPHYSSLSHAVPSPTTGSPLYEHGAQTEIAENQYDVTAHSRL
SSAWTPVAPPSI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable