Gene:
Gene Symbol:
t-b
Gene Name:
T, brachyury homolog
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGATTGATGGTGGTGCAACT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGTGCGACCGAGAG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713743
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1296nts
Open reading frame: 1 to 1296
ATGAGTGCGACCGAGAGCTGCGCCAAGAACGTGCAGTACCGGGTAGATCATCTGCTGAGC
GCTGTGGAGAATGAGCTCCAGGCTGGCAGCGAAAAGGGGGACCCCACCGAGAAGGAGCTG
AAGGTGAGTCTGCAGGAGCGGGACCTGTGGACGAGGTTTAAGGAGCTCACCAATGAGATG
ATCGTCACTAAGAATGGAAGGAGAATGTTTCCAGTTCTGAAGGTGAGCGTGTCGGGCCTG
GATCCCAATGCCATGTACACGGTTCTTCTGGACTTTGTGGCAGCTGACAACCTCCGCTGG
AAGTATGTGAATGGAGAATGGGTTCCAGGTGGCAAACCTGAGCCCCAGGCCCCTAGTTGT
GTTTACATCCACCCAGATTCCCCCAATTTTGGAGCCCACTGGATGAAAGATCCTGTGTCT
TTTAGCAAAGTCAAACTTACAAACAAAATGAATGGTGGAGGCCAGATTATGTTAAACTCT
TTGCACAAGTATGAACCTCGAATCCACATTGTGAGAGTTGGCGGCACCCAGAGAATGATC
ACCAGCCATTCATTCCCTGAGACACAGTTCATAGCAGTGACCGCATACCAGAATGAAGAG
ATTACAGCACTTAAAATCAAGCACAACCCATTTGCCAAAGCATTTCTTGATGCAAAAGAA
AGAAATGATTATAAAGACATCTTGGATGAGGGAATTAATAGTCAGCACTCAAATTTCTCT
CAGTTAGGTACTTGGCTCATTCCTAATGGTGGATCATTATGTACGCCCAATCCACACACT
CAGTTCGGGGCACCTCTCTCTCTATCATCTCCTCATGGCTGTGAGCGATACTCATCTCTG
AGGAACCACCGCTCCGCTCCTTACCCCAGTCCATACACTCACAGAAACAATTCTCCAAAC
AATTTAACAGATAATTCCTCAGCATGTCTTTCAATGCTCCAGTCCCATGGCAACTGGTCC
ACACTTCAAATGCCAGCACACACAGGGATGTTGCCAATGAGTCACAGCACAGGAACACCA
CCTACCTCAAGTCAGTACACTTCTTTGTGGTCTGTAAGTAACAGTACCATCACACCAGTG
TCTCAGTCTGGAGGAATAAGTAATGGAACAAGTCCCCAGTACCTACGTGGCTCTACACCT
CATTACTCATCTCTTTCACATGCTGTGCCCTCACCAACCACAGGATCCCCATTGTATGAA
CATGGAGCACAAACAGAAATAGCAGAAAACCAGTATGATGTCACTGCACATTCCAGGCTT
TCATCAGCATGGACACCAGTTGCACCACCATCAATCTAA
Protein sequence predicted based on nucleotide sequence
MSATESCAKNVQYRVDHLLSAVENELQAGSEKGDPTEKELKVSLQERDLWTRFKELTNEM IVTKNGRRMFPVLKVSVSGLDPNAMYTVLLDFVAADNLRWKYVNGEWVPGGKPEPQAPSC VYIHPDSPNFGAHWMKDPVSFSKVKLTNKMNGGGQIMLNSLHKYEPRIHIVRVGGTQRMI TSHSFPETQFIAVTAYQNEEITALKIKHNPFAKAFLDAKERNDYKDILDEGINSQHSNFS QLGTWLIPNGGSLCTPNPHTQFGAPLSLSSPHGCERYSSLRNHRSAPYPSPYTHRNNSPN NLTDNSSACLSMLQSHGNWSTLQMPAHTGMLPMSHSTGTPPTSSQYTSLWSVSNSTITPV SQSGGISNGTSPQYLRGSTPHYSSLSHAVPSPTTGSPLYEHGAQTEIAENQYDVTAHSRL SSAWTPVAPPSI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin
4339
4958
death cassette
ccdB
ccdB negative selection gene (death cassette), LOST in recombined (with insert) form
1200
1409
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
3020
3040
recombination site
attP1
attP recombination site 1 present in empty vector with death cassette, replaced by attL1 in recombined form with gene of interest or other insert
569
652
recombination site
attP2
attP recombination site 2 present in empty vector with death cassette, replaced by attL2 in recombined form with gene of interest or other insert
2896
2983
selectable marker
SpectR
spectinomycin resistance gene
3471
4262
trxn termination sequence
rrnB
rrnB T1 and T2 terminator
268
470
