Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATGCATAATAACAAGGAGATACCAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGAGCGGGAGAGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713756
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5484nts
Open reading frame: 1 to 5484
ATGGGAGCGGGAGAGGAGCGGCCCGAGGCGCTGGGATTTAATCCCCAGAAGGAAATCGTG
TACAATTTACTCCTTCCCTACGCGCATCGCCTCGACCGGGAATCCAATGAGCTCCTGGCG
CAAATCAAGGGCAGTTTGGGCCGAGCCGTGCGGCTGAGGGAACTCTGGCCTGGAGTTTTG
TTCTGGACTCGCAAACTCACTACGTATATTCGATTATATGGCAGAAAATTCAGTAAAGAA
GATCATGTTCTTTTTGTCAAGTTGCTGTACGAGTTGGTGACAATCCCCAAACTGGAAATT
AGTATGATGCAAGGATTCGCACGCTTGTTAATAAGCCTTTTAAAGAAAAAAGAACTTCTC
TCTAGAGAAGACTTACAGTTGCCCTGGAGACCATTGTACGAAATGCTGGAGAGAATATTA
TATTCTAAAACCGAACACCTCGGACTCAACTGGTTTCCCAATTCTGTCGAGGGCGTCCTG
AAAACTCTAGTGAAAGCCTGTAGACCTTATTTTCCCGACGATGCAACAGCAGAAATGCTA
CAAGAATGGCTGCCTTTAATGTGTCCTTTTGATGTAACAATGCAGAAGGCCATTAGTTAT
CTTGAATTATTTTTGCCTACCTCCCTGCCCCCAGACCTTCACTGCAAAGGTTTTAGGCTT
TGGTTTGATGAATTCTTGACTCTCTGGGTTTCAGTTCAGAATCTTCCACAATGGGAAGGG
CATTTGGTGAATCTTTTCGCTAGACTGGCAAATGACAACATTGGGTACATTGACTGGGAT
CCATATGTACCAAAGATCTTTACCAGAATCCTGCGGAGCTTAAATCTTCCAGTTGGGAGC
AATCAAGTGTTAGTCCCAAGACAACTGGCCAATGCTTATGACATCGGCCATGCGGTGATC
TGGATAACTGCCTTGATGGGTGGACCAAGTAAAACTGTCCAGAAGCATTTAACAGGACTG
TTCAATAGCATCACTTCTTTTTATCATCCGTCTAACAACGGCAGGTGGTTGACAAAGCTC
ATGAAACTGCTGCAGAGGTTGCCGTGTTGCATTATCCGCCGGCTGCATCGTGAACGTTAC
AAGAAACCCTCGTGGTTGACTCCGGTACCTGAAAGTCACAGACTTACTGACCAAGATGTG
ACGGACTTTGTTGAAAGCATTATGCAGCCTGTGCTGTTGGCAATGTTCAGTAAAACTGGA
AGCCTGGAGGCGGCCCAGGCCCTGCAGAATCTTGCACTAATGCGCCCTGAGCTAGTAATC
CCTCCAGTCCTTGAAAAAACTTACCCAGCTTTGGAAACTCTAACTGAGCCTCACCAGCTT
ACAGCAACATTGAGCTGTGTGATTGGAGTGGCCCGCAGCTTGGTGTCTGGCGGAAGGTGG
TTCCCAGAAGGTCCAACACACATGCTACCTCTGCTAATGAGAGCACTTCCCGGTGTGGAT
CCAAACGATTTCAGCAAATGCATGATCACATTCCAGTTTATTGCAACTTTTTCCACGTTG
GTGCCTTTAGTAGATTGTTCATCTTTACTGCAAGAGAGAAACGATCTTTCAGAGGTGGAG
AGGGAGCTGTGCTCTGCTACCGCTGAATTTGAAGACTTTGTGCTCCAGTTCATGGACAGA
TGCTTTGCCCTCATAGAGAGCAGTGCCCTTGAGCAAACTCGAGAAGAGACGGAGACCGAG
AAGATGACCCATTTGGAGAGTCTAGTAGAGCTAGGCCTGTCTTCTACCTTCAGTACAATC
CTCACACAGTGTTCCAAGGAGATATTTAAGGTTGCCCTGGAGAAGGTTTTCAACTTTGCA
GTTTCCAATATATTTGAAACGCGAGTGTCGGGCAGAATGGTTGCCGACTTGTGCCGGGCT
GCTGTCAAGTGTTGCCCTGTGGAATCTTTGAAGCTCTTTCTGCCTCATTGCTGCAATGTT
ATATCTCACCTCACTATTAATGATGATGTGATGAACGATGAAGAACTGGATAAAGAGCTG
CTCTGGAAGCTGCAGCTCTTGTCTGAGATAACAAGAGTTGATGGGGAGAAGCTGCTCCCC
TACAAAGAGCAACTTGTACAGATTCTCCATCGGACGCTGCACTTCACTTGCAAACAGGGT
TACACTTTGTCCTGCAACCTTCTGCACCATCTGCTGCGCTCCAGCACCCTCATCTACCCC
ACAGAATACTGCAGTGTGCCCGGGGGCTTTGATAAGCCGCTCTCTGACTACTTTCCCATC
AAGGACTGGGGAAAACCAGGAGATTTGTGGAACCTAAATATCAAGTGGCACGTTCCCTCA
GCGGAAGAGATGGACTTTGCGTATTATCTGCTGGATACTTTTCTGCGGCCAGAACTACAG
AAGCTGGACCTGTACTCAAGTGGAGAGCTTGAAATGAGCAGGGATGAAGTTCAGCAGTGT
CTGGCCATCGTCCACAATTGTTTGACTGGCTCAGGGAATCTTCTGCCCCCATTGCACGGG
GAAAGAGTAACTCACTTGGTTACGAGCATGGTGTCTTTGAATGAGACAAAACTTTTTACT
GGGATCGACCATGATCATTCTAGAGAAAACTACAGAGAACTAATTTCAAAAACACTCAGG
AAACTGTTGCACTATATTCTTGACCACTCAGAAGATGATACCAAATCTTTATTTCTAATA
ATAAAGATTATCAGTGACCTTCTGCAGTTCCAGGGGTCTCACAAACACGAGTTCGACTCC
AGGTGGAAAAGTTTTACTCTGGTCAAAAAGTCCATGGAGAATCGACTTCATGGGAAGAAA
CGGCACATTCGGGCCCTTCTGATTGACAGAGTGATGCTGCAGCATGAGCTGAGAACACTG
ACCGTGGAAGGCTGCGAATACAAAAAAGTCCACCAGGACATGCTGAGGGATCTGCTGCGG
CTCTCCACCAGTTCTTATGGTCAGGTGAGAAACAAAGCTCAGCAGGCTTTCTTTACTGCT
CTGGGAACTTACAACTTCTGCTGCCGGGACCTGATTCCTCTCGTACTTGAATTCCTGCGA
CCGGAACGACAGGATGTGACGCAGCAACAGTTCAAAGGTGCCTTGTATTGTCTTCTGGGA
AACCACGGCGGGGTCTGTTTAGCAAATCTCCATGACTGGGAGTGTATTGTGCAGACGTGG
CCGGCAATGATCTCTTCAGGGCTCAGCAAAGCCATGTCTCTTGAGAAGCCGTCCATCGTT
CGCCTTTTCGATGACTTGGCAGAGAAGATCCATCGACAGTACGAAACGATTGGCCTGGAT
TTTTCTGTCCCTGAGAAATGCATAGAGATTGCGATCCTGCTGCAGCACGCGGCATCTACC
AGCTCCCAACTGCCCCATCCCGAGGAACTTGCATTAGCGATTAAACGCCAGGGAGAGAAG
AATGTGGAGGCCGTGCAAAACTATGAACGTTTGGTCAACACTTTATTGGACTGTGTTACC
CAAAGAAATCTCCCATGGAAATTTGAGCACATCGGCATTGGTTTTCTGTCTTTGCTCCTG
AGAGACGATTATGTTCTGCCTGTGCGTGCCATCCGGTACTTGGTGCAATGCCTCAATCAC
GACGCTCTCATTGTGCGCAAGATGGCCATCTCCACTGTGGCTGGGATTCTGAAGCAGCTG
AAGAGAACACATGTGAAAGAGACCATCTGTCCTTACAAAATCAGTGGCTGCCCCAAACCA
GAGAGCAAGTTGGTAGGAGACCGGCCGGACAACCAGTGGCTGCTTTACGACAGTAGCAAC
TTGCCAAACACAAAAGAGGCCTGGGAGTCGTGCTGCTTTGTGGAGAAGACACACTGGGGG
TATTCCAGCTGGCCTCAAAATATGTTGGTATATGCACCGGCTGATCAGCAGCCAAAAGTC
GGAAGAAGCAGAGAAGAAATGTCAGAGGCAGAACAAATCATTTATGATCATTTCACTGAT
GAGAAGTTTGTTGACCAACTGATAAAGTTTCTGTCTTTAGAAGACCGAAAAGGAAAAGAC
AAATTTAATCCCCGCAGGTTTTGCCTCTTCAAGGGACTTTTCAGAAACTATGATGATGCA
TTTTTGCCAATAATAAAACCCCATTTGGAGCGCCTGGTTGCAGATTCGCATGAAAGCACC
CAGCGATGTGCGGCGGAAATCGTTGCCGGCTTGATCAGGGGCTCCAAACATTGGACGTTT
GAAAAGGTGGAAAACCTCTGGAATTTTTTGTGTGAGCTTTTGAGAACTGCCCTATCCAAC
ATCACAGTAGAAACGTACAGTGACTGGGGAACCTGCATCGCTACATCTTGTGAAAGCCGA
GATCCTCGAAAACTGCACTGGTTGTTTGAGTTGCTGCTGGAGTCTCCAGTGAGTGGGGAA
GGAGGATCCTTTGTGGATGCCTGCCGACTATATGTGCTCCAGGGAGGCCTGGCCCAGCAG
GAGTGGAGAGTTCCAGAACTACTACATAGATTGCTTATGTGTCTGGAACCTAAACTTACT
CAAGTCTATAAAAATGTGCGGGAGCGGATTGGAAGTGTTCTGACCTATATATTCATGATC
GATGTCTCCTTGCCAAACACTGCGCCGACAAAGTCCCCTCACATCTCTGACTTTACCGGC
CGCATCCTTGGCAAACTCAAGCCGCTCATGGACGCCGATGAAGAGATACAGAACCACGTC
ATGGAAGAAAATGGAGTTGGGGAACAAGATGAGAGAACCCAGGCAATTAAATTGATGAAG
ACAATATTAAAATGGATAATGGCAAGTGCTGGGAGATCCTTCTGTACAGGTGTTACGGAA
CAGATGCAGCTTTTGCCGTTGCTTTTTAAGATTGCACCTGTAGAAAATGACACCAACTAT
GATGAACTTAAAAGAGACGCAAAAACGTGTCTGTCCCTCATGTCGCAAGGTTTATTGCTC
CCAGTGCAGGTGCCGCTGGTGCTCGATGTGCTCAGACAAACAGCAAGAAGCAGCTCTTGG
CATGCTAGATACACTGTTCTGACCTACATCCAGACGATGGTCTTCTATAATCTCTTTATT
TTCATTCACAATGAAGAGTCAGTGCAAGGTGTGCGGTGGTTGATCCTGCAACTCATGGAA
GATGAGCAACTCGAGGTGAGAGAAATGGCTGCTACAACACTGAGTGGCTTGCTGCAGTGC
AACTTCCTGACAATGGATGCCGCCATGCAGGCACATTTTGAGGCGCTGTGTAAAACCAGA
CTTCCTAAAAAGAGGAAAAGGGAGAGCGGCATGGTTGGGGACACAATACCTTCCGGAGAT
CTGGTGAAACGTCACGCCGGTGTATTGGGACTCAGCGCATGCATTTTATCCAGTCCTTAT
GATGTACCAACATGGATGCCCCAACTCCTGATGGATCTGAGTGTGCACCTGAATGACCCC
CAGCCCATAGAGATGACTGTGAAGAAAACTCTGTCGAACTTTAGAAGAACTCACCATGAT
AACTGGCAAGAGCACAAGCAGCAATTCACAGACGACCAGTTAATCGTTTTAACAGATCTA
TTGGTATCTCCTTGTTATTATGCATAG
Protein sequence predicted based on nucleotide sequence
MGAGEERPEALGFNPQKEIVYNLLLPYAHRLDRESNELLAQIKGSLGRAVRLRELWPGVL FWTRKLTTYIRLYGRKFSKEDHVLFVKLLYELVTIPKLEISMMQGFARLLISLLKKKELL SREDLQLPWRPLYEMLERILYSKTEHLGLNWFPNSVEGVLKTLVKACRPYFPDDATAEML QEWLPLMCPFDVTMQKAISYLELFLPTSLPPDLHCKGFRLWFDEFLTLWVSVQNLPQWEG HLVNLFARLANDNIGYIDWDPYVPKIFTRILRSLNLPVGSNQVLVPRQLANAYDIGHAVI WITALMGGPSKTVQKHLTGLFNSITSFYHPSNNGRWLTKLMKLLQRLPCCIIRRLHRERY KKPSWLTPVPESHRLTDQDVTDFVESIMQPVLLAMFSKTGSLEAAQALQNLALMRPELVI PPVLEKTYPALETLTEPHQLTATLSCVIGVARSLVSGGRWFPEGPTHMLPLLMRALPGVD PNDFSKCMITFQFIATFSTLVPLVDCSSLLQERNDLSEVERELCSATAEFEDFVLQFMDR CFALIESSALEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKEIFKVALEKVFNFA VSNIFETRVSGRMVADLCRAAVKCCPVESLKLFLPHCCNVISHLTINDDVMNDEELDKEL LWKLQLLSEITRVDGEKLLPYKEQLVQILHRTLHFTCKQGYTLSCNLLHHLLRSSTLIYP TEYCSVPGGFDKPLSDYFPIKDWGKPGDLWNLNIKWHVPSAEEMDFAYYLLDTFLRPELQ KLDLYSSGELEMSRDEVQQCLAIVHNCLTGSGNLLPPLHGERVTHLVTSMVSLNETKLFT GIDHDHSRENYRELISKTLRKLLHYILDHSEDDTKSLFLIIKIISDLLQFQGSHKHEFDS RWKSFTLVKKSMENRLHGKKRHIRALLIDRVMLQHELRTLTVEGCEYKKVHQDMLRDLLR LSTSSYGQVRNKAQQAFFTALGTYNFCCRDLIPLVLEFLRPERQDVTQQQFKGALYCLLG NHGGVCLANLHDWECIVQTWPAMISSGLSKAMSLEKPSIVRLFDDLAEKIHRQYETIGLD FSVPEKCIEIAILLQHAASTSSQLPHPEELALAIKRQGEKNVEAVQNYERLVNTLLDCVT QRNLPWKFEHIGIGFLSLLLRDDYVLPVRAIRYLVQCLNHDALIVRKMAISTVAGILKQL KRTHVKETICPYKISGCPKPESKLVGDRPDNQWLLYDSSNLPNTKEAWESCCFVEKTHWG YSSWPQNMLVYAPADQQPKVGRSREEMSEAEQIIYDHFTDEKFVDQLIKFLSLEDRKGKD KFNPRRFCLFKGLFRNYDDAFLPIIKPHLERLVADSHESTQRCAAEIVAGLIRGSKHWTF EKVENLWNFLCELLRTALSNITVETYSDWGTCIATSCESRDPRKLHWLFELLLESPVSGE GGSFVDACRLYVLQGGLAQQEWRVPELLHRLLMCLEPKLTQVYKNVRERIGSVLTYIFMI DVSLPNTAPTKSPHISDFTGRILGKLKPLMDADEEIQNHVMEENGVGEQDERTQAIKLMK TILKWIMASAGRSFCTGVTEQMQLLPLLFKIAPVENDTNYDELKRDAKTCLSLMSQGLLL PVQVPLVLDVLRQTARSSSWHARYTVLTYIQTMVFYNLFIFIHNEESVQGVRWLILQLME DEQLEVREMAATTLSGLLQCNFLTMDAAMQAHFEALCKTRLPKKRKRESGMVGDTIPSGD LVKRHAGVLGLSACILSSPYDVPTWMPQLLMDLSVHLNDPQPIEMTVKKTLSNFRRTHHD NWQEHKQQFTDDQLIVLTDLLVSPCYYA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
