flrt3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATGAATGTGAATGATCAGAATCTGG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACTACGGACACTTGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713775
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1944nts
Open reading frame: 1 to 1944

ATGACTACGGACACTTGGAATTTATTTCTTGCATGGGCTCAGCTTTTACTATTATTCAGA
ATGTCGCCACAATGTGACGGTGCCAAACCCTGCCCTTCGGTGTGTCGCTGTGATGCAGGC
TTCATTTATTGCAATGATAGAGACTTGACATCCATTCCTTCGGGTATACCAGATGACGCC
ACAACCCTCTACCTTCAAAATAATCAGATCAATAACGCCGGCATACCTTCTGATTTGCGA
GGTCTGGAAAAAGTCGAGAGAATCTACTTGTACCGCAACAGTTTAGATGAGTTTCCAATA
AACCTCCCTAAAAATGTCAAAGAATTGCACCTGCAGGAAAACAATATACGGACTATAACC
TACGATGCACTTTCACAGATTCCTTCCATAGAAGAGCTGCATCTGGATGATAATTCTGTA
TCTGCAGTCAGTATAGAGGATGGAGCTTTTCGGGACAACATCTTTCTGCGGCTCCTGTTC
CTTTCAAGAAATCATCTAAGCACAATACCATGGGGCCTGCCCAGAACAATCGAAGAACTA
CGTTTGGATGATAACCGGATTTCCATCATCTCCGAGATCTCTTTGCAAGACCTCACCAAT
TTGAAACGTCTTGTCCTGGATGGGAACCTTTTAAATAATAATGGTCTCGGTGAAAGGGTC
TTCATGAACTTGATCAATCTGACAGAACTCTCATTAGTACGTAATTCTCTTACCTCTCCT
CCTGCAAACCTGCCTGGCACCAACCTGAGAAAGCTTTACCTTCAAGAAAACCATATGAAC
TATGTACCACCTAATTCGTTTGCTGATTTAACACAACTCTACCGTCTTGATATGTCCAAT
AACAATCTCACAGCTTTACCTCAGGGTATATTTGATGACTTGGATAATCTAACCCAGTTA
TTCCTTCGTAACAACCCTTGGTACTGCGGTTGTAAAATGAAGTGGGTGCGTGACTGGTTG
CAATCTCTGCCTTCCAAAGTTAATGTCCGTGGGTTGATGTGCCAAGCTCCAGAGAGAGTA
AGAGGAATGACCATCAAAGACCTTAACAAAGAACTGTTTGATTGTAAAGACAGGATTGGT
TCGAATACAATACATGTTACAACAACAGTGTTAAACTCTTTGCTTCCAGCACAAGGCCAG
TGGCCTGTGCCTGTAACCAAACAACCTGAAATCAGACCTCCAGATATCAATAAAATCTTT
CGGACAACGCCAATACCCGTAAAAAAAATTATTACGATTCAGGTGAAATCAATAACAACA
GAGACTATCTACATCTCCTGGAAAGTGGCCCTGCCAATGACTGCTTTGAGACTCAGCTGG
CAACTGGGTCATAGTCCTGTTTTTGGATCTATAACGGAAACCATTGTGACAGGTGACAGG
ACAGAGTATTTGCTTACAGCTCTTGAGCCAGAGTCACCATATCGAATATGTATGGTTCCC
ATGGAAACCGGCAACATCTACCTTTCCGATGAAACCCCTGTTTGCATTGAAACAGAGACT
GCTCCACTTAAAATGTACAACCCAACCACAACATTGAACAGAGAGCAAGAGAAGGAGCCT
TATAAGAACTCGAATTTACCATTAGCTGCAATCATTGGGGGAGCCGTGGCTCTAGTAGCT
ATAACACTCCTTGCTTTGGTGTGTTGGTACGTACACAGGAATGGGTCTCTGTTTTCCAGG
AACTGTGCCTACAGCAAGGGTCGCAGAAGAAAAGATGACTATGCAGAAGCTGGCACAAAG
AAGGACAATTCCATTTTAGAAATCAGGGAGACTTCTTTTCCGATGATACCAATAAATAGT
GACCCAATGTCCAAGGAGGAGTTTATAATACACACTATATTTCCACCCAATGGAGTGAGC
CTGTACAAAAACAGTCACAGTGAGAGCAGTAGTAACAGAAGTTACAGAGACAGTGGTATA
CCAGATTCTGATCATTCACATTCATGA

Protein sequence predicted based on nucleotide sequence

MTTDTWNLFLAWAQLLLLFRMSPQCDGAKPCPSVCRCDAGFIYCNDRDLTSIPSGIPDDA
TTLYLQNNQINNAGIPSDLRGLEKVERIYLYRNSLDEFPINLPKNVKELHLQENNIRTIT
YDALSQIPSIEELHLDDNSVSAVSIEDGAFRDNIFLRLLFLSRNHLSTIPWGLPRTIEEL
RLDDNRISIISEISLQDLTNLKRLVLDGNLLNNNGLGERVFMNLINLTELSLVRNSLTSP
PANLPGTNLRKLYLQENHMNYVPPNSFADLTQLYRLDMSNNNLTALPQGIFDDLDNLTQL
FLRNNPWYCGCKMKWVRDWLQSLPSKVNVRGLMCQAPERVRGMTIKDLNKELFDCKDRIG
SNTIHVTTTVLNSLLPAQGQWPVPVTKQPEIRPPDINKIFRTTPIPVKKIITIQVKSITT
ETIYISWKVALPMTALRLSWQLGHSPVFGSITETIVTGDRTEYLLTALEPESPYRICMVP
METGNIYLSDETPVCIETETAPLKMYNPTTTLNREQEKEPYKNSNLPLAAIIGGAVALVA
ITLLALVCWYVHRNGSLFSRNCAYSKGRRRKDDYAEAGTKKDNSILEIRETSFPMIPINS
DPMSKEEFIIHTIFPPNGVSLYKNSHSESSSNRSYRDSGIPDSDHSHS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable