aldh3a2 (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTTTTGCTTATAACACATCCTCTG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAAGCAAATAGTGGACAAAGCA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713937
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1518nts
Open reading frame: 1 to 1518

ATGAAGCAAATAGTGGACAAAGCAAGGAAAGCCTTTCTCTCAGGGAAGACACGTTCTATA
GACTTCAGAATTCAGCAACTGCAAGCCATGAAAAGAATGCTCACTGAAAATCAAGCTGGC
TTTGTTGAAGCACTGAAAGCAGACTTGCACAAGAATGAATGCACTGTTTACAGCTATGAG
ATTATGGGAATGCTTGGTGAAATTGACTTGGCCCTTGACAATCTCCCAAAGTGGACAGAG
CCAAAGCACGTTAAAAAGAACATAATGACTATGCGTGATGAAGTGTATATTAATTATGAA
CCTCTTGGCGTTGTCCTGGTAATTGGAGCTTGGAACTATCCTGTGGTGGTTCTTTTGCAA
CCTGTAGTGGGGGCCATAGCAGCAGGCAATGCTGCAGTAATAAAGCCTTCAGAAGTCAGT
GAGAACACTGCCAAGCTTTTGGAGAAACTCATCCCTCGCTATATGGATAAGGAGCTGTAC
CCTGTAGTAAATGGAGGGATTGCCGAAACTACTGAGCTGCTTGCACAGAGATTTGACCAC
ATCTGCTATACAGGCAATACTGGTGTTGGCAAAATAATAATGTCAGCTGCTGCCAAGTTC
CTTACCCCAGTCACCCTTGAACTAGGAGGGAAGAGTCCATGTTATATAGATAACAACTGT
GATATTGACATAGCTAGTAGACGTATTACATGGGGAAAGTTTGTTAACTGCGGCCAGACT
TGCATAGCTCCTGATTATATTCTGTGTGACAAGTCCATCCAGGATAAATTAGTGGAAAAG
ATTAAGGAAACACTTAAGGAATTTTACGGTGAAGATGTCAAGAAATCTCCAGATTATGAA
AGGATTATAAACAAGCGCCATTTTAAGCGCATTCTTGAATTGATGGAAGGACAGAATATA
GTTCATGGAGGGAGTTATGATGAAGGAACTTGTTATATAGAACCAACAGTCATGGTGGAT
GTGAATCCAGAATCCAAGGTTATGCAAGAAGAGATTTTTGGGCCCCTGCTTCCCATTGTA
TCAGTGAGAAATTTAGATGAAGCCATTCAGTTCATAAATGATAGAGAAAAACCTCTTGCC
CTCTATGTTTTCTCTAAAAATAAACAGGTGATCAAAAGAATGATTTCTGAGACTTCCAGT
GGTGGTGTCACTGCCAATGATGTCATAATGCATTTCACAGTTCAAGAATTACCATTTGGA
GGAGTTGGTCACAGTGGAATGGGAGCCTATCATGGAAAACACACCTTTGATACTTTCTCA
CACAAGCGGGCATGCCTTATTAAGTCTCTTGCTATGGAAGGTGTAAACAAGATCAGGTAT
CCCCCGTACAGTATGAAAAAGGTGGAATGGGCCAAATTTCTTTTAACAACTAAGCTGAGC
AAGAAGAAGCTTGGTTTGGTGCTTGTACCCATGTTTGCCATACTGGCAGCTGTTATTCTG
AAGAGGTATCGATGGCAGCTGGCAGGCAGAGGGATGCAACTTATGCTGGCATATCAGAGG
ATGTGTTATAAGCAAAACTGA

Protein sequence predicted based on nucleotide sequence

MKQIVDKARKAFLSGKTRSIDFRIQQLQAMKRMLTENQAGFVEALKADLHKNECTVYSYE
IMGMLGEIDLALDNLPKWTEPKHVKKNIMTMRDEVYINYEPLGVVLVIGAWNYPVVVLLQ
PVVGAIAAGNAAVIKPSEVSENTAKLLEKLIPRYMDKELYPVVNGGIAETTELLAQRFDH
ICYTGNTGVGKIIMSAAAKFLTPVTLELGGKSPCYIDNNCDIDIASRRITWGKFVNCGQT
CIAPDYILCDKSIQDKLVEKIKETLKEFYGEDVKKSPDYERIINKRHFKRILELMEGQNI
VHGGSYDEGTCYIEPTVMVDVNPESKVMQEEIFGPLLPIVSVRNLDEAIQFINDREKPLA
LYVFSKNKQVIKRMISETSSGGVTANDVIMHFTVQELPFGGVGHSGMGAYHGKHTFDTFS
HKRACLIKSLAMEGVNKIRYPPYSMKKVEWAKFLLTTKLSKKKLGLVLVPMFAILAAVIL
KRYRWQLAGRGMQLMLAYQRMCYKQN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable