rad17 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTCACTGTCATACTCTTCAATCT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTCAAAAACATCTTGCAGAAGG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713982
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2022nts
Open reading frame: 1 to 2022

ATGTCAAAAACATCTTGCAGAAGGAAGGTAACCTCCGCTAAGATTACTGACTGGGTTGAA
CCATCTTTTGAAGACTTCTTTGGAAACACAAGCAAAGCTTCATCTCAGACATCAGGAATT
GTTAAAAGATGCGCCCAAACAAAGAATGAATCTTCCTTAGAGAACAAACAGGGAAAGAAG
AGAATCAATCCATTTACAAAAGACCATGCTAGCGATAATAATCAACATACTTTTGACTAC
TCCCAGAATGATCCCTGGATTGATAAATACAGACCAGAAATTCAGGCAGAACTTGCCGTG
CACAAGAAAAAAATTGAAGAGGTTGAGGCCTGGTTGAAAACCCATGTGGAGAAGAGGCCA
CAGAAGCAGGGGGGTCAGATTTTATTACTAACTGGGCCCCCGGGCTGTGGAAAGACTGCA
ACAATTCAAGTGCTTACTAAAGAAATGGGGATTCAGGTACAGGAATGGATCAATCCTCTA
ATGCAGGAGTTCAAACAAGATCTACCAGAAGTTTTTGATAGAGATACGAGGTTTCAGCCT
TTCACCAGTCAGTCTCAGACATCTCTCTTTCACGACTTTCTCTTAAGGGCAAATAAATAC
AACAAACTACAGATGGTTGGTGAATCTTTAAGCACGGACAGGAAAATCATTGTTGTAGAT
GATATGCCTAATCAGTTTTATCGAGACCCCTCTGCTTTACATGATATTCTTAGGAGGTTT
GTAAAAACAGGCCGCTGCCCTCTAGTGTTCATAATATCAGACAGCCTGAGTGGGGAGAGC
CATCAACGGAGACTTTTCCCGAAGGAAATCCAAGAAGAACTAGTAGTTAACAATATAAGT
TTCAACCCTGTAGCCCCAACAACCATGACAAAAGTTTTGACCCGAATTGCTAGCACAGAG
GCAAATGCAAGTGGTGGAAAAATTATCATTCCTGATAAAGCATCACTAGAGTTAATCTGT
ACTGGCAGTTCAGGGGATATCCGAAGCGCAATTAATAGTCTTCAGTTTTCAGCTCGGCAA
GGTTCATCTCTGAAAAATGATAGCTGGTCTAAAAGCAGAGGGAAAGCATCAAAATCAGGA
AAACCCTCTTCCAAATCAAAGACTAAAAAGGAGCCACGGAAAGGTGGAGAAAATGTAGAT
GAAGTACAAGCAATTGGTGGGAAAGATGCTTCACTCTTTTTATTCCGAGCCTTGGGGAAA
ATACTTTACTGCAAACGAGACCCCGTACCAGATGATACAGATGTTTATCGTTTGCCTGCT
CACTTATCTGAATATAACAGAGACTTGCTTCTCATCCTTCCAGAGGCCGTTGTGGAAAAG
TCTCACATGAATGGAGAACTGTTCAATTTATATCTTCACCAGAATTATTTGGAATTCTTC
ATGGATATTGATGATGTAGTCCGAGCAAGCGAATATCTAAGCAGTGCCGATGTGCTCTCT
GGTGATTGGAATTCCCGTTCCACACTCAGTGTGTACAGTTCTTCAGTGGCAAGCAGAGGG
ATCATACATTCTAACAGTGCCAGAGCTTTTGTCAACTGTCAAGGAGGAGTTGGGTTCAAG
CCACTACATAAACCTCAGTGGCTTGCAATAAATAAAAAGCACCAAGAAAATTGTCTTGCT
GCAAGGTCTCTCTTCTCAACCTTCTGTTCCACACCGCTTGGTCTTCAAACTCAGGTGGTA
CCGTATCTGGCAATGCTCACAAATCCATTGAGGAATCCATCACAAATTGCATTTATTCAA
GATGTTGGCCGGCTACCGTTAAAAAGGCAGTTTGGAAGGCTAAAACTTGAAGCACTCACA
GACAAAGATTCTGGACTCCCAGATGGCGAGAGTGGTGAGGAAGATAACCTTACTGAAATG
CAAATAGAAAGAAATGACGCTGGCAATTCCATGGCTGATAGAAAGGAACTGTTGTCCAGT
CAAGGACTGGAAAGTGAGTTGCCAGCCAGTCAGCCCCAACCAGTTGCAGCACAAGCAATT
ATGGAGGATGAAGAGTTAAAGATTGAAGAGTATGACAGTGACTGA

Protein sequence predicted based on nucleotide sequence

MSKTSCRRKVTSAKITDWVEPSFEDFFGNTSKASSQTSGIVKRCAQTKNESSLENKQGKK
RINPFTKDHASDNNQHTFDYSQNDPWIDKYRPEIQAELAVHKKKIEEVEAWLKTHVEKRP
QKQGGQILLLTGPPGCGKTATIQVLTKEMGIQVQEWINPLMQEFKQDLPEVFDRDTRFQP
FTSQSQTSLFHDFLLRANKYNKLQMVGESLSTDRKIIVVDDMPNQFYRDPSALHDILRRF
VKTGRCPLVFIISDSLSGESHQRRLFPKEIQEELVVNNISFNPVAPTTMTKVLTRIASTE
ANASGGKIIIPDKASLELICTGSSGDIRSAINSLQFSARQGSSLKNDSWSKSRGKASKSG
KPSSKSKTKKEPRKGGENVDEVQAIGGKDASLFLFRALGKILYCKRDPVPDDTDVYRLPA
HLSEYNRDLLLILPEAVVEKSHMNGELFNLYLHQNYLEFFMDIDDVVRASEYLSSADVLS
GDWNSRSTLSVYSSSVASRGIIHSNSARAFVNCQGGVGFKPLHKPQWLAINKKHQENCLA
ARSLFSTFCSTPLGLQTQVVPYLAMLTNPLRNPSQIAFIQDVGRLPLKRQFGRLKLEALT
DKDSGLPDGESGEEDNLTEMQIERNDAGNSMADRKELLSSQGLESELPASQPQPVAAQAI
MEDEELKIEEYDSD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable