zbtb8a.1-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTCTTGTTTTTCTTTCAACTCATC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGAATTCTCTTCCCATCAC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714042
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1410nts
Open reading frame: 1 to 1410

ATGGAATTCTCTTCCCATCACATTCGTCTTCTGCAGCAGTTGGATGAACAGCGGCAGAAG
GATCTCTTTTGTGACTGTCACATTATAGTGGAAGGGCAGATGTTTAAAGCACATCGTAAT
GTACTGTTTGCCAGTAGTGGTTACTTCAAAATGCTTCTGTCTCAAAGCTGCAGGGACATG
GGTGAGCCAATCACAGCCACCTTTGATGTGTTTTCTGCTGACACGTTTACAGCCATCCTA
GACTTTGTTTATTCAGGCAAATTGCCTCTTTCTGGACAAAATGTTATTGAGGTTATGTCT
GCAGCCAGCTATCTTCAAATGACCGATGTTATTGGTGTTTGCAAAATGTTTATCAAATCC
TCCTTGGACATAAATGAGAAGGACAGAGATGGTTTCTTCAGCCTTTCAGACAAGGATGCT
GGCAGTAATGGTTCTGGGCTTTATGCTGCAGGTTGGAGGACTGAGAGCAGCCCAACACAT
ACACATGATACAGCAGAGCATGGCAGCTTTATTGCTGGTTATAATTACCCCCCTCCAATT
ACCTCACGGTTGCAGCGCCCTTTCTCTAAGCACCCACGCAAACCTGAGCTTGTTCGCAAG
CATCGTAGGCGCCTACTGCCAGAACCTCTGACGCCAGCACCACTCAGTCATATTCCACTG
GGTGACTTGGTGGGAGGCTCAGCAGAATGTATGCTGCATGATGAGGAAACAGTTGAGAGT
GTGTCACAGGAAGAGGAAAGAACCCAAACACAGGAGTCCATAATAAGTATTAAAGATGAG
GATGAAGATGCTGCATCTCACAGCTGGCCTGAATCTCCTCAGCAAGAGTCTCTGGACCAG
GGTCCAGCTCTTCATATAACCAAGGCAGAAGAGCTGTACAAGGCAATGCCTACCATGCTG
GGAGGAGTATCTGGATGGGGTGAAGATGAACTAAGTTCTGGGAGGTTTAAATGCCCTTTC
TGCACCCATACCGTGAAACGTAAAGCCGATCTAAAACGTCACCTGCGATGCCACACAGGA
GAGCGACCTTACCCATGTGAAGCCTGTGGCAAGAGGTTCACTAGACTGGAGCACTTGCGC
AATCACTTTCAGACAATTCATGAGGCTGGTAAACTAATCTGTCGAAGGTGCAAACTTCCA
GTAACCAAAGTAACCGGTCGTGTTATTCAGGATGGGACAAGGAGATACCGCCTTTGCCAT
GCATGTCTGGCAGAAGCTGGCTTGGATAACGTCAACTTTGACTATGGAGAAGACCAGCCA
TTGGTGTTACCACCTGAAAATGAGAGGGAACATTGCTGGAATTTCAAGGAAGAAGGAAGA
AAGGAAAATGGGAGTGAGCGTGCAGAGTCAGACCTAGCTATACAGGAGGTGGTAGACAGC
GAAGATGATGAGTTGAAAGAAAAACAAGACTAA

Protein sequence predicted based on nucleotide sequence

MEFSSHHIRLLQQLDEQRQKDLFCDCHIIVEGQMFKAHRNVLFASSGYFKMLLSQSCRDM
GEPITATFDVFSADTFTAILDFVYSGKLPLSGQNVIEVMSAASYLQMTDVIGVCKMFIKS
SLDINEKDRDGFFSLSDKDAGSNGSGLYAAGWRTESSPTHTHDTAEHGSFIAGYNYPPPI
TSRLQRPFSKHPRKPELVRKHRRRLLPEPLTPAPLSHIPLGDLVGGSAECMLHDEETVES
VSQEEERTQTQESIISIKDEDEDAASHSWPESPQQESLDQGPALHITKAEELYKAMPTML
GGVSGWGEDELSSGRFKCPFCTHTVKRKADLKRHLRCHTGERPYPCEACGKRFTRLEHLR
NHFQTIHEAGKLICRRCKLPVTKVTGRVIQDGTRRYRLCHACLAEAGLDNVNFDYGEDQP
LVLPPENEREHCWNFKEEGRKENGSERAESDLAIQEVVDSEDDELKEKQD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable