cpe (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAAAATTCAAGGTCTGTGACATCAT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTCAGGAATAGGCTCTTC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714486
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1392nts
Open reading frame: 1 to 1392

ATGTCAGGAATAGGCTCTTCCCTGGGTCTCTCACTGGTTGTGCTGCTGCTTCTCGGCTGC
TCCACTTCCCTGACCCGGGGTCGCAGGCTGAGCCGGGAGCCCGGGGACATCTCGTTCGAG
TATCACCGTTACCCGGAGCTGCGCGACGCGCTGGTGTCTGTGTGGCTGCAGTGTCCTTCC
ATCAGTCGCATCTACACCGTGGGACGGAGCTTTGAGGGCCGGGAGCTGCTCGTCATAGAG
ATCTCCGACAACCCCGGGGAGCACGAGCCCGGTGAACCAGAATTTAAATATGTTGGCAAT
ATGCATGGAAACGAAGCTGTTGGAAGAGAGCTGCTTATATATTTAGCCCAGTATTTGTGC
AATGAGTACCAGAGGGAAAATGAAACCATTGTCAATCTGATCCATAACACTCGTATCCAC
ATTATGCCTTCTCTGAATCCCGATGGCTTTGAGAAAGCCGCCCAGCAGCCTGGAGAGATA
AAAGACTGGTTTGTAGGACGTAGTAACGCTCAGAGCATAGACCTGAACAGAAACTTCCCT
GATCTTGATCGCATTGTGTACTTAAATGAGCGAGAAGGAGGCACAAACAATCATCTGATG
CAAAACCTGAAGAAGTCTGTTGATGAAAACACAAAGCTTGCTCCAGAAACAGTAGCTGTA
ATTCACTGGATTATGGATATCCCCTTTGTCCTCTCTGCAAACCTTCATGGAGGAGATATA
GTTGCAAACTATCCATATGACAAGACACGCAGCGGAAGTGCACATGAATACAGTGCATGC
CCAGATGACAGTATTTTCAAAAGCTTAGCTCGTGCCTACTCATCCCTTAACCCTACCATG
TCAGACCCAGATAGGAAGCCATGCCGCAAATCTGACGATGACACCAGTTTCATTGACGGG
ACAACAAATGGTGCAGCTTGGTATAGTGTGCCTGGAGGTATGCAAGATTTTAATTACTTA
AGCAGCAATTGTTTTGAGATAACCGTTGAGCTTGGTTGTGACAAGTTTCCTCAAGAAGAA
AAGCTGAAGTCCTACTGGGAGGAAAATAAAGACTCTCTAGTGAACTATTTGACGCAGGTG
CACCGTGGTATAACAGGATTTGTCAAAGATCACCATGGTTTTCCAATTGCCAATGCTACT
ATATCTGTTGATGGAATTGACCATGATGTGACTTCAGCAAAGGACGGTGACTACTGGAGA
CTGCTGACACCTGGAAATTATAAAATTACAGTTTCAGCACCTGGGTATCTGGCTGTTGTG
AAGAAAGTAGCTGTTCCTTATAGCCCAGCTGTTAAGGTTGACTTTGACCTTGAGTCCTTG
ACAGAAAGAAAGGAGGAAGAGAAGGAAGAGATGATGGAATGGTGGAAGATGATGTCACAG
ACCTTGAATTTTTAA

Protein sequence predicted based on nucleotide sequence

MSGIGSSLGLSLVVLLLLGCSTSLTRGRRLSREPGDISFEYHRYPELRDALVSVWLQCPS
ISRIYTVGRSFEGRELLVIEISDNPGEHEPGEPEFKYVGNMHGNEAVGRELLIYLAQYLC
NEYQRENETIVNLIHNTRIHIMPSLNPDGFEKAAQQPGEIKDWFVGRSNAQSIDLNRNFP
DLDRIVYLNEREGGTNNHLMQNLKKSVDENTKLAPETVAVIHWIMDIPFVLSANLHGGDI
VANYPYDKTRSGSAHEYSACPDDSIFKSLARAYSSLNPTMSDPDRKPCRKSDDDTSFIDG
TTNGAAWYSVPGGMQDFNYLSSNCFEITVELGCDKFPQEEKLKSYWEENKDSLVNYLTQV
HRGITGFVKDHHGFPIANATISVDGIDHDVTSAKDGDYWRLLTPGNYKITVSAPGYLAVV
KKVAVPYSPAVKVDFDLESLTERKEEEKEEMMEWWKMMSQTLNF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable