dsc3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  dsc3
Gene Name:  desmocollin 3
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACTTTTTTGCACAGACTTCTGCTA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGACTGACACTGACA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714593
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2709nts
Open reading frame: 1 to 2709

ATGGGACTGACACTGACATCCACAGGGACGTCCCTTCTTGCCTTGTGCCTGTTACTCACC
CTGCCAGTAGCTGTGTTTACAGACACATGTGAGAAAGTGAAACTGCATGTACCTTCTGTA
ATCTATTCTGGAAATCTGATCGGCAAAGTAAATCCTCGGCATTGTCTGGATTCAGATTCA
CTTATCATTCAGTCAAGCAATCCTGACTTCTTAGTTTTGGAAAATGGATCAATTTATGCG
CAAAAACGTATTTCACTGGCTGGTAAAACATGGTTTACAATTCGAATCTTGGACTCCGTG
ACTCTGACTGAGAAAAAAATTCGAGTTAAACTGATAACCAAAGCAAAGCCTGCTAAATCA
AGATATGCAAGAGAGCTACTGAGACGTTCCAAACGAAGATGGGCCCCTCTACCATTTTCT
ATCATGGAAAGCTATATTGGACCATTCCCATGTTTTGTACAACAGATCCAGTCTGATACA
CAGGAGAACTATACAATCAGATACTCTATATCAGGACCTGGCGTAAATGAAGAACCCTTT
GACTTGTTCTACGTTGAACCCACTACTGGAAATATATATTATAGAAGAATGGTTGATCGT
GAGCAGTATCCAGTTTTTAACTTAATTGGCTTTGCAAACACGTTGGATGGCTATTCGCCA
GAAGCTCCTTTAAATATAGTAATCAAAGTTGAAGACAGCAATGATAATGCACCTATATTT
GTAGAAGAAGCATTTTGTATTGAAGTTGCTGAACACACAAAACAAGGTGTAATAATAGGA
AGAGTCAAGGCCTTGGACAAGGATGAACCCAATACCCTGCACACTACCCTCAGATATAGT
ATTATTTCACAGACTCCTCCATCACCTGTAATGTTTGCTCTTCATCCAGAAACTGGGATT
ATTACTACAACCTCAAACAGACTTGATCGAGAGACCATAGAGTCTTATGTCCTTATCATT
GGAGTTCGGGATATGCCTGGACAGCGTTCATTTTTGTCCTCGACTGGAACAGTATCCATA
ACTGTTACGGATGTAAATGACAATCCACCCACTTTTGTGCAAAAGTCTTACCAGGTAGAA
GTTAATGAAAATGAAAGTGGAATGGTCCTCTTATGCATACCAATAACAGACAATGACATT
CGCGACTCTCCCAATTGGAGAGCTAAGTATTACATCACTCAGGGAAATGAGAGAGAATAT
TTCAGTATCGGTACAAATCCTGAAACCAATGAGGGATGTCTCAAGGTCGAAAAGGGACTA
AATTATGAGGAAACAAAAAGGATTCAAATACTGGTGGGAGTCACCAATGAAGTTGATGTT
ATAAACTATTCAGGGGTGAAAAGTTCTGGCATGAGCACCATCCCTGTCACCATAATTGTT
AAAGATGTAGATGAAGGACCTGAGTTCATACCAACTATCAAAGAGATAAGAGTGAGAGAA
AACACACCAGTAGGGACTGAAATATATAGATGTCAAGCAAAAGACCCAGAAACGAAGAAC
AGTGATGGCATACAGTTTTCGGAACAGTTGGATACACTTAACTGGGTAAATATTGATGCA
ACAACCTGTCGCATAACAATAGCGAGAATCCTGGATTATGAAACGGATCAGGCACCAAGA
CACCAGCATAATGTGACTATAATTGGAGTAGATCAGAGCGGCAAGACAGGCACTGGAACA
CTTGTAATATATCTGGAAGATGTTAATGATAACTATCCAAACATTTCAAAGGCTGACACA
ACTGTTTGCCAGAGTGGTAGAACCTATAGTCTGGTTGTGGCCGAAGACCGAGATGGAGTT
CCTAACAGTGCACCTTTCGTATTCCAAATTGATTCCACCAATATCCCCAACCTGCAAAAT
CAGTTTAAAATAGAAAGAATTAATGATACAGCCATTTATATAAGAGCCGATGGGGTTCCA
GATGGAGATTACCAAATTCCACTAATCATAACAGATCAGCAAGGATATGGCCAAACACAG
ATTATGAATATTAGGAAATGCTATTGCCCTGATAATGAGAACTGTGTTGGTAATATAAGA
TCTTCAAACAATGTTGCCCTTGGTGGATGGGCTATTCTAGTGATGGTGCTGGCTGCATTA
CTCTTTGCCCTGCTCTTATGTGGTTTGTGTGCTTGCCTGTGTGGTGCCGCAGCTACAAAG
GGTAAAAAAAGTTTTCCTGATGATTCAGCACAACAGAATCTAATTGTAACCAACACTGAG
GCACCAGGAGCAGATGTGATGGATTCAAATTTCAAAGTTCCTGTTCATGTAACAGATGCA
CACCGTAGTGGAAATGCTCCATCTGTTTCAGGTGGCAAAGGACAAAGTGGAAGCCAAATT
ATACAACAAACTATCCATACAAGAAAAACCACAGGGCAACTAAACACTGCAGAGGATAAC
CGAGGAGGCCTTACCTTAACTTCTATGGGTGGAGGAGGACAACAACAGTTCTTTGATGTG
AACAGAGTAAACTATACAGACTGGCATAGTTTTATGAACAACCATCTTGATGAAAAATTA
TACATGTGTGGACAGAATGAGGAACAACAACATGGAGATGATTATGTTCTGTCATACAAC
TTTGAAGGAAAAGGGTCTGCGGCAGGATCTGTTGGATGCTGCAGTGACTTTCGAGATGAG
GACAGAATGGACTTTTTAAATAATTTAGAACCAAAGTTCAGGACATTAGCAGAAGTCTGT
GCAAAAAAGTAA

Protein sequence predicted based on nucleotide sequence

MGLTLTSTGTSLLALCLLLTLPVAVFTDTCEKVKLHVPSVIYSGNLIGKVNPRHCLDSDS
LIIQSSNPDFLVLENGSIYAQKRISLAGKTWFTIRILDSVTLTEKKIRVKLITKAKPAKS
RYARELLRRSKRRWAPLPFSIMESYIGPFPCFVQQIQSDTQENYTIRYSISGPGVNEEPF
DLFYVEPTTGNIYYRRMVDREQYPVFNLIGFANTLDGYSPEAPLNIVIKVEDSNDNAPIF
VEEAFCIEVAEHTKQGVIIGRVKALDKDEPNTLHTTLRYSIISQTPPSPVMFALHPETGI
ITTTSNRLDRETIESYVLIIGVRDMPGQRSFLSSTGTVSITVTDVNDNPPTFVQKSYQVE
VNENESGMVLLCIPITDNDIRDSPNWRAKYYITQGNEREYFSIGTNPETNEGCLKVEKGL
NYEETKRIQILVGVTNEVDVINYSGVKSSGMSTIPVTIIVKDVDEGPEFIPTIKEIRVRE
NTPVGTEIYRCQAKDPETKNSDGIQFSEQLDTLNWVNIDATTCRITIARILDYETDQAPR
HQHNVTIIGVDQSGKTGTGTLVIYLEDVNDNYPNISKADTTVCQSGRTYSLVVAEDRDGV
PNSAPFVFQIDSTNIPNLQNQFKIERINDTAIYIRADGVPDGDYQIPLIITDQQGYGQTQ
IMNIRKCYCPDNENCVGNIRSSNNVALGGWAILVMVLAALLFALLLCGLCACLCGAAATK
GKKSFPDDSAQQNLIVTNTEAPGADVMDSNFKVPVHVTDAHRSGNAPSVSGGKGQSGSQI
IQQTIHTRKTTGQLNTAEDNRGGLTLTSMGGGGQQQFFDVNRVNYTDWHSFMNNHLDEKL
YMCGQNEEQQHGDDYVLSYNFEGKGSAAGSVGCCSDFRDEDRMDFLNNLEPKFRTLAEVC
AKK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable